Restriction map for
pFN21AE9526
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AvaII AviII
AvrII BamHI BbvCI BclI BfrI
BglI BglII BlnI BsaBI BseAI
BsiEI BsiHKAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HinfI
KpnI MamI MfeI MflI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PleI
PmeI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcsI 911 GAATTT [GA]^AATT[CT]
AgeI 805 ACCGGT A^CCGGT
AhdI 286 GACGGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 911 GAATTT [GA]^AATT[CT]
Asp700I 489 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 286 GACGGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1185 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1087 CTCGGG C^[CT]CG[GA]G
BanII 670 GGGCTC G[GA]GC[CT]^C
BbeI 1249 GGCGCC GGCGC^C
BbrPI 177 CACGTG CAC^GTG
BcgI 1171 CGAAGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 816 GGATAC GTATCC(6/5)
BfaI 710 CTAG C^TAG
BlpI 609 GCTGAGC GC^T[ACGT]AGC
BpmI 552 CTCCAG CTGGAG(16/14)
Bpu1102I 609 GCTGAGC GC^T[ACGT]AGC
BsaAI 177 CACGTG [CT]AC^GT[GA]
BsaWI 805 ACCGGT [AT]^CCGG[AT]
BseRI 1053 CTCCTC GAGGAG(10/8)
BsmBI 298 GAGACG CGTCTC(1/5)
BsmFI 167 GGGAC GGGAC(10/14)
BsoBI 1087 CTCGGG C^[CT]CG[GA]G
BspMI 31 ACCTGC ACCTGC(4/8)
BstAPI 772 GCATGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 586 CCATCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 609 GCTGAGC GC^T[ACGT]AGC
DraIII 829 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1266 CCGCGG C^C[GA][CT]GG
Eam1105I 286 GACGGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 448 CTCTTC CTCTTC(1/4)
HaeII 1249 GGCGCC [GA]GCGC^[CT]
HincII 927 GTTAAC GT[CT]^[GA]AC
HindII 927 GTTAAC GT[CT]^[GA]AC
HpaI 927 GTTAAC GTT^AAC
KasI 1249 GGCGCC G^GCGCC
Ksp632I 448 CTCTTC CTCTTC(1/4)
KspI 1266 CCGCGG CCGC^GG
MaeI 710 CTAG C^TAG
MslI 519 CATCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1249 GGCGCC GG^CGCC
PflFI 1185 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 805 ACCGGT A^CCGGT
PmaCI 177 CACGTG CAC^GTG
PmlI 177 CACGTG CAC^GTG
PshAI 1302 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1266 CCGCGG CCGC^GG
ScaI 629 AGTACT AGT^ACT
SfoI 1249 GGCGCC GGC^GCC
TaqI 317 TCGA T^CGA
Tth111I 1185 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 317 TCGA T^CGA
XmnI 489 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BcnI 339 CCCGG CC^[GC]GG
1200 CCGGG
BsmI 70 GAATGC GAATGC(1/-1)
59 GCATTC
BsrFI 805 ACCGGT [GA]^CCGG[CT]
1175 GCCGGT
BsrI 957 CCAGT ACTGG(1/-1)
1281 CCAGT
Cfr10I 805 ACCGGT [GA]^CCGG[CT]
1175 GCCGGT
Cfr13I 747 GGGCC G^G[ACGT]CC
1101 GGGCC
DpnII 357 GATC ^GATC
738 GATC
DraII 746 GGGGCCT [GA]G^G[ACGT]CC[CT]
1100 AGGGCCT
EcoO109I 746 GGGGCCT [GA]G^G[ACGT]CC[CT]
1100 AGGGCCT
HphI 375 GGTGA GGTGA(8/7)
63 TCACC
MaeIII 211 GTCAC ^GT[ACGT]AC
284 GTGAC
MboI 357 GATC ^GATC
738 GATC
MseI 865 TTAA T^TAA
928 TTAA
MspA1I 1003 CCGCTG C[AC]G^C[GT]G
1266 CCGCGG
NciI 339 CCCGG CC^[GC]GG
1200 CCGGG
NdeII 357 GATC ^GATC
738 GATC
PflMI 1017 CCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1098 CCAGGGCCTGG
Sau3AI 357 GATC ^GATC
738 GATC
Sau96I 747 GGGCC G^G[ACGT]CC
1101 GGGCC
Tru9I 865 TTAA T^TAA
928 TTAA
Tsp45I 211 GTCAC ^GT[GC]AC
284 GTGAC
Van91I 1017 CCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1098 CCAGGGCCTGG
XcmI 383 CCATTTATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
518 CCATCTCAGTGCTGG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI BalI BanI BbsI BbvI
BmyI BpuAI BsaHI BsaI BsaJI
BsgI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI DdeI EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MaeII MboII MluNI MnlI MscI
MspI MvaI MvnI MwoI NlaIII
NlaIV PstI RsaI ScrFI SfaNI
SfcI TseI Tsp509I TspRI