Restriction map for
pFN21AE6436
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BbrPI
BciVI BfrI BglII BlnI BpmI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraI EaeI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 1128 ACACGT A^C[GA][CT]GT
AocI 616 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1248 AGCGCT AGC^GCT
BamHI 383 GGATCC G^GATCC
BbeI 1162 GGCGCC GGCGC^C
BbsI 1357 GTCTTC GAAGAC(2/6)
BbvCI 1102 GCTGAGG CCTCAGC(-5/-2)
BcgI 108 CGAGGTAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 764 TGATCA T^GATCA
BfaI 895 CTAG C^TAG
BpuAI 1357 GTCTTC GAAGAC(2/6)
BsaI 59 GAGACC GGTCTC(1/5)
BsmAI 59 GAGAC GTCTC(1/5)
Bsp1407I 1052 TGTACA T^GTACA
BsrGI 1052 TGTACA T^GTACA
BstXI 297 CCAGCATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 616 CCTCAGG CC^T[ACGT]AGG
DrdI 1138 GACTGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 748 GAAGAG CTCTTC(1/4)
Eco47III 1248 AGCGCT AGC^GCT
Eco81I 616 CCTCAGG CC^T[ACGT]AGG
FbaI 764 TGATCA T^GATCA
HgaI 643 GACGC GACGC(5/10)
KasI 1162 GGCGCC G^GCGCC
Ksp632I 748 GAAGAG CTCTTC(1/4)
MaeI 895 CTAG C^TAG
NarI 1162 GGCGCC GG^CGCC
PpuMI 1198 GGGACCC [GA]G^G[AT]CC[CT]
SfiI 87 GGCCTCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1162 GGCGCC GGC^GCC
SmaI 1232 CCCGGG CCC^GGG
SspBI 1052 TGTACA T^GTACA
Tsp509I 351 AATT ^AATT
XmaCI 1232 CCCGGG C^CCGGG
XmaI 1232 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 643 GACGCC G[GA]^CG[CT]C
1162 GGCGCC
ApaI 1071 GGGCCC GGGCC^C
1350 GGGCCC
AvaI 56 CCCGAG C^[CT]CG[GA]G
1232 CCCGGG
BanII 1071 GGGCCC G[GA]GC[CT]^C
1350 GGGCCC
BlpI 393 GCTGAGC GC^T[ACGT]AGC
820 GCTGAGC
Bpu1102I 393 GCTGAGC GC^T[ACGT]AGC
820 GCTGAGC
BsaAI 838 TACGTG [CT]AC^GT[GA]
1129 CACGTA
BsaHI 643 GACGCC G[GA]^CG[CT]C
1162 GGCGCC
BseRI 40 GAGGAG GAGGAG(10/8)
913 GAGGAG
BsoBI 56 CCCGAG C^[CT]CG[GA]G
1232 CCCGGG
BspMI 62 ACCTGC ACCTGC(4/8)
539 ACCTGC
BsrDI 465 CATTGC GCAATG(2/0)
873 CATTGC
BssSI 182 CACGAG CACGAG(-5/-1)
652 CTCGTG
BstYI 383 GGATCC [GA]^GATC[CT]
974 GGATCT
CelII 393 GCTGAGC GC^T[ACGT]AGC
820 GCTGAGC
DraIII 562 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1338 CACCCTGTG
DsaI 101 CCACGG C^C[GA][CT]GG
1037 CCACGG
EcoNI 63 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
954 CCTTGATGAGG
EcoT14I 702 CCTTGG C^C[AT][AT]GG
776 CCTTGG
Hin1I 643 GACGCC G[GA]^CG[CT]C
1162 GGCGCC
HphI 1217 TCACC GGTGA(8/7)
1361 TCACC
MflI 383 GGATCC [GA]^GATC[CT]
974 GGATCT
MslI 439 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
952 CACCTTGATG
PleI 478 GACTC GAGTC(4/5)
185 GAGTC
StuI 620 AGGCCT AGG^CCT
1205 AGGCCT
StyI 702 CCTTGG C^C[AT][AT]GG
776 CCTTGG
TfiI 19 GAATC G^A[AT]TC
308 GAATC
XcmI 443 CCAGTGCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1226 CCATGTCCCGGGTGG
XhoII 383 GGATCC [GA]^GATC[CT]
974 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbvI BcnI BglI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI