Restriction map for
pFN21AE2280
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BfrI BlnI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 280 GTAGAC GT^[AC][GT]AC
AcsI 727 AAATTC [GA]^AATT[CT]
AflIII 509 ACGTGT A^C[GA][CT]GT
AlwNI 910 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 727 AAATTC [GA]^AATT[CT]
AspHI 1038 GTGCTC G[AT]GC[AT]^C
BalI 893 TGGCCA TGG^CCA
BamHI 480 GGATCC G^GATCC
BbvCI 961 GCTGAGG CCTCAGC(-5/-2)
BfaI 100 CTAG C^TAG
BglI 1018 GCCAGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 503 AGATCT A^GATCT
BlpI 157 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 157 GCTGAGC GC^T[ACGT]AGC
BsaAI 508 TACGTG [CT]AC^GT[GA]
BsaI 292 GAGACC GGTCTC(1/5)
BsiHKAI 1038 GTGCTC G[AT]GC[AT]^C
BsmAI 292 GAGAC GTCTC(1/5)
BsmI 957 GAATGC GAATGC(1/-1)
BstAPI 1031 GCATCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 157 GCTGAGC GC^T[ACGT]AGC
DraIII 73 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 575 CCATGG C^C[GA][CT]GG
EaeI 893 TGGCCA [CT]^GGCC[GA]
EcoNI 685 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 590 AAGCTT A^AGCTT
MaeI 100 CTAG C^TAG
MaeII 509 ACGT A^CGT
MluNI 893 TGGCCA TGG^CCA
MscI 893 TGGCCA TGG^CCA
NcoI 575 CCATGG C^CATGG
NspI 150 GCATGC [GA]CATG^[CT]
PflMI 72 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 599 GAGTC GAGTC(4/5)
SexAI 684 ACCTGGT A^CC[AT]GGT
SmaI 866 CCCGGG CCC^GGG
SmlI 419 CTCAAG C^T[CT][GA]AG
SphI 150 GCATGC GCATG^C
StuI 670 AGGCCT AGG^CCT
TaqI 821 TCGA T^CGA
TfiI 438 GATTC G^A[AT]TC
Tsp509I 728 AATT ^AATT
TthHB8I 821 TCGA T^CGA
Van91I 72 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 866 CCCGGG C^CCGGG
XmaI 866 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AfaI 84 GTAC GT^AC
384 GTAC
ApaI 4 GGGCCC GGGCC^C
1025 GGGCCC
BanII 4 GGGCCC G[GA]GC[CT]^C
1025 GGGCCC
BbsI 680 GAAGAC GAAGAC(2/6)
544 GTCTTC
BpuAI 680 GAAGAC GAAGAC(2/6)
544 GTCTTC
BsrBI 214 GAGCGG CCGCTC(-3/-3)
271 GAGCGG
HinfI 438 GATTC G^A[ACGT]TC
599 GAGTC
PpuMI 48 GGGACCC [GA]G^G[AT]CC[CT]
985 GGGACCC
RsaI 84 GTAC GT^AC
384 GTAC
XcmI 460 CCAGCTGGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
476 CCAAGGATCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanI BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
XhoII