Restriction map for
pFN21AE6446
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BssHII Bst1107I BstAPI BstBI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MroI MseI MunI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfuI SmaI SmlI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 508 GGTACC G^GTACC
AlwNI 7 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 858 CCTGAGG CC^T[ACGT]AGG
ApaI 199 GGGCCC GGGCC^C
Asp718I 508 GGTACC G^GTACC
AspI 1063 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 622 CCCGAG C^[CT]CG[GA]G
BbeI 1036 GGCGCC GGCGC^C
BbrPI 93 CACGTG CAC^GTG
BbvCI 306 CCTCAGC CCTCAGC(-5/-2)
BcgI 624 CGAGACACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 93 CACGTG [CT]AC^GT[GA]
BsmBI 441 CGTCTC CGTCTC(1/5)
BsoBI 622 CCCGAG C^[CT]CG[GA]G
Bsp1407I 719 TGTACA T^GTACA
BspLU11I 1196 ACATGT A^CATGT
BspMI 630 ACCTGC ACCTGC(4/8)
BsrBI 1303 GAGCGG CCGCTC(-3/-3)
BsrDI 123 GCAATG GCAATG(2/0)
BsrGI 719 TGTACA T^GTACA
BssSI 991 CTCGTG CACGAG(-5/-1)
BstEII 63 GGTGACC G^GT[ACGT]ACC
BstPI 63 GGTGACC G^GT[ACGT]ACC
Bsu36I 858 CCTGAGG CC^T[ACGT]AGG
DraIII 796 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 858 CCTGAGG CC^T[ACGT]AGG
EcoNI 1427 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 63 GGTGACC G^GT[ACGT]ACC
FseI 890 GGCCGGCC GGCCGG^CC
KasI 1036 GGCGCC G^GCGCC
KpnI 508 GGTACC GGTAC^C
NarI 1036 GGCGCC GG^CGCC
NdeI 782 CATATG CA^TATG
NheI 901 GCTAGC G^CTAGC
PflFI 1063 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1100 GACTC GAGTC(4/5)
PmaCI 93 CACGTG CAC^GTG
PmlI 93 CACGTG CAC^GTG
SexAI 956 ACCTGGT A^CC[AT]GGT
SfoI 1036 GGCGCC GGC^GCC
SgrAI 403 CGCCGGCG C[GA]^CCGG[CT]G
SphI 652 GCATGC GCATG^C
SspBI 719 TGTACA T^GTACA
TfiI 332 GATTC G^A[AT]TC
Tth111I 1063 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 423 CGCG CG^CG
1287 CGCG
AflIII 94 ACGTGT A^C[GA][CT]GT
1196 ACATGT
AspHI 184 GTGCTC G[AT]GC[AT]^C
988 GTGCTC
BcnI 872 CCGGG CC^[GC]GG
1151 CCGGG
BfaI 868 CTAG C^TAG
902 CTAG
BsaI 151 GAGACC GGTCTC(1/5)
595 GAGACC
BseRI 823 CTCCTC GAGGAG(10/8)
1428 CTCCTC
BsiEI 657 CGGCCG CG[GA][CT]^CG
889 CGGCCG
BsiHKAI 184 GTGCTC G[AT]GC[AT]^C
988 GTGCTC
BsrFI 404 GCCGGC [GA]^CCGG[CT]
891 GCCGGC
BstUI 423 CGCG CG^CG
1287 CGCG
BstYI 530 GGATCT [GA]^GATC[CT]
1308 GGATCT
Cfr10I 404 GCCGGC [GA]^CCGG[CT]
891 GCCGGC
DsaI 59 CCATGG C^C[GA][CT]GG
1073 CCATGG
EagI 657 CGGCCG C^GGCCG
889 CGGCCG
EarI 1102 CTCTTC CTCTTC(1/4)
1228 CTCTTC
EclXI 657 CGGCCG C^GGCCG
889 CGGCCG
Eco52I 657 CGGCCG C^GGCCG
889 CGGCCG
EcoT14I 59 CCATGG C^C[AT][AT]GG
1073 CCATGG
HaeII 671 AGCGCC [GA]GCGC^[CT]
1036 GGCGCC
HinfI 332 GATTC G^A[ACGT]TC
1100 GACTC
Ksp632I 1102 CTCTTC CTCTTC(1/4)
1228 CTCTTC
MaeI 868 CTAG C^TAG
902 CTAG
MaeII 94 ACGT A^CGT
1386 ACGT
MflI 530 GGATCT [GA]^GATC[CT]
1308 GGATCT
MvnI 423 CGCG CG^CG
1287 CGCG
NaeI 404 GCCGGC GCC^GGC
891 GCCGGC
NciI 872 CCGGG CC^[GC]GG
1151 CCGGG
NcoI 59 CCATGG C^CATGG
1073 CCATGG
NgoMIV 404 GCCGGC G^CCGGC
891 GCCGGC
PstI 1255 CTGCAG CTGCA^G
1474 CTGCAG
SfcI 1255 CTGCAG C^T[GA][CT]AG
1474 CTGCAG
StuI 342 AGGCCT AGG^CCT
568 AGGCCT
StyI 59 CCATGG C^C[AT][AT]GG
1073 CCATGG
TaqI 433 TCGA T^CGA
686 TCGA
TthHB8I 433 TCGA T^CGA
686 TCGA
XcmI 269 CCAGCCTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
781 CCATATGCCATGTGG
XhoII 530 GGATCT [GA]^GATC[CT]
1308 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BalI BanI BanII BbsI
BbvI BmyI BpmI BpuAI BsaHI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI