Restriction map for
pFN21AE3658
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BbvI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
Fnu4HI FseI FspI HgaI Hin1I
HindIII HpaI ItaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MflI MluI MroI MseI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I TseI Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwI 99 GATCC GGATC(4/5)
AlwNI 330 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 71 GGGCCC GGGCC^C
AvaI 127 CCCGGG C^[CT]CG[GA]G
AvaII 240 GGACC G^G[AT]CC
BalI 416 TGGCCA TGG^CCA
BbsI 148 GTCTTC GAAGAC(2/6)
BclI 272 TGATCA T^GATCA
BpuAI 148 GTCTTC GAAGAC(2/6)
BsaAI 94 TACGTG [CT]AC^GT[GA]
BsaI 32 GAGACC GGTCTC(1/5)
BsgI 170 GTGCAG GTGCAG(16/14)
BsmAI 32 GAGAC GTCTC(1/5)
BsmFI 372 GTCCC GGGAC(10/14)
BsoBI 127 CCCGGG C^[CT]CG[GA]G
BstF5I 448 GGATG GGATG(2/0)
Cac8I 133 GCTGGC GC[ACGT]^[ACGT]GC
DraII 71 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 416 TGGCCA [CT]^GGCC[GA]
EcoO109I 71 GGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 374 CCCGC CCCGC(4/6)
FbaI 272 TGATCA T^GATCA
FokI 448 GGATG GGATG(9/13)
HapII 128 CCGG C^CGG
HincII 226 GTCAAC GT[CT]^[GA]AC
HindII 226 GTCAAC GT[CT]^[GA]AC
HinfI 431 GAATC G^A[ACGT]TC
HpaII 128 CCGG C^CGG
MaeII 95 ACGT A^CGT
MaeIII 349 GTCAC ^GT[ACGT]AC
MluNI 416 TGGCCA TGG^CCA
MscI 416 TGGCCA TGG^CCA
MspI 128 CCGG C^CGG
MwoI 368 GCAAGTCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 244 CATG CATG^
PvuII 355 CAGCTG CAG^CTG
SfaNI 449 GATGC GCATC(5/9)
SmaI 127 CCCGGG CCC^GGG
TfiI 431 GAATC G^A[AT]TC
Tsp45I 349 GTCAC ^GT[GC]AC
XcmI 354 CCAGCTGAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 127 CCCGGG C^CCGGG
XmaI 127 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 51 CCGC CCGC(-3/-1)
375 CCGC
AluI 235 AGCT AG^CT
356 AGCT
AspHI 64 GAGCAC G[AT]GC[AT]^C
340 GAGCAC
BanII 19 GAGCCC G[GA]GC[CT]^C
71 GGGCCC
BcnI 127 CCCGG CC^[GC]GG
128 CCGGG
BpmI 141 CTCCAG CTGGAG(16/14)
29 CTGGAG
BseRI 110 CTCCTC GAGGAG(10/8)
138 CTCCTC
BsiHKAI 64 GAGCAC G[AT]GC[AT]^C
340 GAGCAC
BsiYI 22 CCCAAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
127 CCCGGGGCTGG
BslI 22 CCCAAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
127 CCCGGGGCTGG
BsrI 68 ACTGG ACTGG(1/-1)
143 CCAGT
CfoI 189 GCGC GCG^C
424 GCGC
DpnII 99 GATC ^GATC
273 GATC
EcoT14I 54 CCTTGG C^C[AT][AT]GG
398 CCAAGG
HaeII 188 AGCGCC [GA]GCGC^[CT]
423 GGCGCT
HhaI 189 GCGC GCG^C
424 GCGC
HinP1I 189 GCGC G^CGC
424 GCGC
MboI 99 GATC ^GATC
273 GATC
MspA1I 355 CAGCTG C[AC]G^C[GT]G
375 CCGCTG
NciI 127 CCCGG CC^[GC]GG
128 CCGGG
NdeII 99 GATC ^GATC
273 GATC
Sau3AI 99 GATC ^GATC
273 GATC
StyI 54 CCTTGG C^C[AT][AT]GG
398 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AfaI BmyI BsaJI Bsp1286I BssKI
BstNI Cfr13I DdeI DsaI EcoRII
HaeIII HphI MboII MnlI MvaI
NlaIV RsaI Sau96I ScrFI TspRI