Restriction map for
pFN21AE4317
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI AlwI AlwNI AocI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AvrII BalI
BamHI BbsI BciVI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaI BseAI BseRI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoRI EcoT14I EcoT22I FseI HindIII
HpaI KpnI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI ScaI
SexAI SfuI SmaI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 363 GACGTC GACGT^C
AccI 190 GTCGAC GT^[AC][GT]AC
AfaI 915 GTAC GT^AC
Alw44I 580 GTGCAC G^TGCAC
Aor51HI 545 AGCGCT AGC^GCT
ApaI 9 GGGCCC GGGCC^C
ApaLI 580 GTGCAC G^TGCAC
AscI 345 GGCGCGCC GG^CGCGCC
AviII 446 TGCGCA TGC^GCA
BanI 493 GGCGCC G^G[CT][GA]CC
BbeI 493 GGCGCC GGCGC^C
BbrPI 102 CACGTG CAC^GTG
BbvCI 525 GCTGAGG CCTCAGC(-5/-2)
BcgI 182 GCATGGCGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 887 TGATCA T^GATCA
BsaAI 102 CACGTG [CT]AC^GT[GA]
BsaWI 896 ACCGGA [AT]^CCGG[AT]
BsmBI 626 CGTCTC CGTCTC(1/5)
BssSI 289 CTCGTG CACGAG(-5/-1)
BstEII 69 GGTGACC G^GT[ACGT]ACC
BstPI 69 GGTGACC G^GT[ACGT]ACC
BstXI 563 CCAAGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 714 CCGCGG C^C[GA][CT]GG
EarI 535 CTCTTC CTCTTC(1/4)
Eco47III 545 AGCGCT AGC^GCT
EcoO65I 69 GGTGACC G^GT[ACGT]ACC
EcoRV 652 GATATC GAT^ATC
FauI 257 CCCGC CCCGC(4/6)
FbaI 887 TGATCA T^GATCA
FspI 446 TGCGCA TGC^GCA
HgaI 517 GACGC GACGC(5/10)
HincII 190 GTCGAC GT[CT]^[GA]AC
HindII 190 GTCGAC GT[CT]^[GA]AC
KasI 493 GGCGCC G^GCGCC
Ksp632I 535 CTCTTC CTCTTC(1/4)
KspI 714 CCGCGG CCGC^GG
MboII 536 TCTTC GAAGA(8/7)
NarI 493 GGCGCC GG^CGCC
NotI 149 GCGGCCGC GC^GGCCGC
PleI 697 GAGTC GAGTC(4/5)
PmaCI 102 CACGTG CAC^GTG
PmlI 102 CACGTG CAC^GTG
PstI 751 CTGCAG CTGCA^G
RsaI 915 GTAC GT^AC
SacII 714 CCGCGG CCGC^GG
SalI 190 GTCGAC G^TCGAC
SapI 534 GCTCTTC GCTCTTC(1/4)
SbfI 750 CCTGCAGG CCTGCA^GG
SfaNI 661 GATGC GCATC(5/9)
SfcI 751 CTGCAG C^T[GA][CT]AG
SfoI 493 GGCGCC GGC^GCC
SmlI 226 CTCAAG C^T[CT][GA]AG
Sse8387I 750 CCTGCAGG CCTGCA^GG
TfiI 789 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 103 ACGTGT A^C[GA][CT]GT
623 ACACGT
AspHI 280 GAGCAC G[AT]GC[AT]^C
580 GTGCAC
BanII 9 GGGCCC G[GA]GC[CT]^C
174 GGGCTC
BcnI 13 CCCGG CC^[GC]GG
145 CCCGG
BsiHKAI 280 GAGCAC G[AT]GC[AT]^C
580 GTGCAC
BsmAI 627 GTCTC GTCTC(1/5)
699 GTCTC
BsmFI 683 GGGAC GGGAC(10/14)
107 GTCCC
BspMI 470 ACCTGC ACCTGC(4/8)
753 GCAGGT
BstF5I 459 CATCC GGATG(2/0)
600 CATCC
DraII 8 GGGGCCC [GA]G^G[ACGT]CC[CT]
9 GGGCCCC
EcoO109I 8 GGGGCCC [GA]G^G[ACGT]CC[CT]
9 GGGCCCC
FokI 459 CATCC GGATG(9/13)
600 CATCC
HinfI 697 GAGTC G^A[ACGT]TC
789 GATTC
MaeIII 70 GTGAC ^GT[ACGT]AC
644 GTGAC
MslI 261 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
840 CATCGTGGTG
NciI 13 CCCGG CC^[GC]GG
145 CCCGG
SfiI 142 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
712 GGCCGCGGCGGCC
SgrAI 489 CGCCGGCG C[GA]^CCGG[CT]G
639 CGCCGGTG
Tsp45I 70 GTGAC ^GT[GC]AC
644 GTGAC
TspRI 160 CAGTG CA[GC]TG[ACGT][ACGT]^
352 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BbvI BglI BmyI BsaHI BsaJI
BsgI BsiEI BsiYI BslI BsoBI
Bsp1286I BsrFI BsrI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII EaeI EagI
EclXI Eco52I EcoRII Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NdeII NgoMIV NlaIII
NlaIV Sau3AI Sau96I ScrFI TaqI
TseI TthHB8I