Restriction map for
pFN21AE6138
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Cac8I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HinfI HpaI HphI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AfaI 57 GTAC GT^AC
AocI 256 CCTGAGG CC^T[ACGT]AGG
AvaII 397 GGACC G^G[AT]CC
BbvI 216 GCTGC GCAGC(8/12)
BsaI 261 GGTCTC GGTCTC(1/5)
BsmFI 396 GGGAC GGGAC(10/14)
BspMI 151 GCAGGT ACCTGC(4/8)
BsrI 193 CCAGT ACTGG(1/-1)
BstF5I 374 GGATG GGATG(2/0)
BstNI 69 CCTGG CC^[AT]GG
BstYI 294 AGATCC [GA]^GATC[CT]
Bsu36I 256 CCTGAGG CC^T[ACGT]AGG
DraII 396 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 256 CCTGAGG CC^T[ACGT]AGG
EcoO109I 396 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 179 GAATTC G^AATTC
EcoRII 69 CCTGG ^CC[AT]GG
EcoT14I 269 CCTTGG C^C[AT][AT]GG
FokI 374 GGATG GGATG(9/13)
HapII 39 CCGG C^CGG
HindIII 319 AAGCTT A^AGCTT
HpaII 39 CCGG C^CGG
MboII 27 GAAGA GAAGA(8/7)
MflI 294 AGATCC [GA]^GATC[CT]
MspI 39 CCGG C^CGG
MvaI 69 CCTGG CC^[AT]GG
MwoI 5 GCCGCGTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 396 GGGACC GG[ACGT]^[ACGT]CC
PpuMI 396 GGGACCT [GA]G^G[AT]CC[CT]
PstI 217 CTGCAG CTGCA^G
RsaI 57 GTAC GT^AC
ScaI 56 AGTACT AGT^ACT
SfaNI 165 GCATC GCATC(5/9)
SmaI 38 CCCGGG CCC^GGG
StyI 269 CCTTGG C^C[AT][AT]GG
TseI 216 GCTGC G^C[AT]GC
XhoII 294 AGATCC [GA]^GATC[CT]
XmaCI 38 CCCGGG C^CCGGG
XmaI 38 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 179 GAATTC [GA]^AATT[CT]
378 GAATTT
AlwI 295 GATCC GGATC(4/5)
273 GGATC
ApoI 179 GAATTC [GA]^AATT[CT]
378 GAATTT
AvaI 38 CCCGGG C^[CT]CG[GA]G
392 CTCGGG
BcnI 38 CCCGG CC^[GC]GG
39 CCGGG
BsiYI 193 CCAGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
364 CCTACAGTTGG
BslI 193 CCAGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
364 CCTACAGTTGG
BsmAI 242 GAGAC GTCTC(1/5)
262 GTCTC
BsoBI 38 CCCGGG C^[CT]CG[GA]G
392 CTCGGG
CfoI 66 GCGC GCG^C
96 GCGC
HhaI 66 GCGC GCG^C
96 GCGC
HinP1I 66 GCGC G^CGC
96 GCGC
MaeIII 160 GTCAC ^GT[ACGT]AC
355 GTCAC
NciI 38 CCCGG CC^[GC]GG
39 CCGGG
SfcI 217 CTGCAG C^T[GA][CT]AG
365 CTACAG
Tsp45I 160 GTCAC ^GT[GC]AC
355 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AccII AciI AluI BsaJI BseRI
BssKI BstUI Cfr13I DdeI DpnII
Fnu4HI HaeIII ItaI MboI MnlI
MvnI NdeII Sau3AI Sau96I ScrFI
Tsp509I