Restriction map for
pFN21AE5213
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BglI BlnI BmyI BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FauI FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflII 454 CTTAAG C^TTAAG
AhdI 9 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 13 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 9 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 29 TGCGCA TGC^GCA
BcnI 155 CCCGG CC^[GC]GG
BfaI 166 CTAG C^TAG
BfrI 454 CTTAAG C^TTAAG
BglII 404 AGATCT A^GATCT
BlpI 699 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 699 GCTGAGC GC^T[ACGT]AGC
BsaWI 311 TCCGGT [AT]^CCGG[AT]
BseRI 459 GAGGAG GAGGAG(10/8)
BsgI 480 CTGCAC GTGCAG(16/14)
BsmAI 208 GAGAC GTCTC(1/5)
BsmFI 153 GTCCC GGGAC(10/14)
BsmI 449 GCATTC GAATGC(1/-1)
Bsp1407I 321 TGTACA T^GTACA
BspMI 647 ACCTGC ACCTGC(4/8)
BsrGI 321 TGTACA T^GTACA
BstAPI 12 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 225 CCACCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 699 GCTGAGC GC^T[ACGT]AGC
CfoI 30 GCGC GCG^C
Eam1105I 9 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 177 GAAGAG CTCTTC(1/4)
EcoRV 130 GATATC GAT^ATC
FspI 29 TGCGCA TGC^GCA
HgaI 9 GACGC GACGC(5/10)
HhaI 30 GCGC GCG^C
HinP1I 30 GCGC G^CGC
HinfI 159 GAATC G^A[ACGT]TC
Ksp632I 177 GAAGAG CTCTTC(1/4)
MaeI 166 CTAG C^TAG
MaeII 34 ACGT A^CGT
MseI 455 TTAA T^TAA
MspA1I 680 CCGCTG C[AC]G^C[GT]G
NciI 155 CCCGG CC^[GC]GG
NheI 165 GCTAGC G^CTAGC
PflMI 53 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SspBI 321 TGTACA T^GTACA
TaqI 123 TCGA T^CGA
TfiI 159 GAATC G^A[AT]TC
Tru9I 455 TTAA T^TAA
Tsp509I 105 AATT ^AATT
TthHB8I 123 TCGA T^CGA
Van91I 53 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AocI 240 CCTGAGG CC^T[ACGT]AGG
669 CCTCAGG
BbvI 497 GCAGC GCAGC(8/12)
27 GCTGC
BpmI 537 CTCCAG CTGGAG(16/14)
60 CTGGAG
BsrI 432 ACTGG ACTGG(1/-1)
366 CCAGT
Bsu36I 240 CCTGAGG CC^T[ACGT]AGG
669 CCTCAGG
Cac8I 165 GCTAGC GC[ACGT]^[ACGT]GC
394 GCAAGC
Eco81I 240 CCTGAGG CC^T[ACGT]AGG
669 CCTCAGG
MaeIII 530 GTGAC ^GT[ACGT]AC
620 GTGAC
MslI 33 CACGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
226 CACCATGATG
PpuMI 236 GGGTCCT [GA]G^G[AT]CC[CT]
509 AGGACCT
SfaNI 3 GATGC GCATC(5/9)
664 GATGC
SmlI 283 CTCAAG C^T[CT][GA]AG
454 CTTAAG
TseI 27 GCTGC G^C[AT]GC
497 GCAGC
Tsp45I 530 GTGAC ^GT[GC]AC
620 GTGAC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BsaJI BsiYI BslI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TspRI
XhoII