Restriction map for
pFN21AE4429
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AviII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BpmI BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1247 GGTACC G^GTACC
AccII 1269 CGCG CG^CG
AcsI 1285 GAATTT [GA]^AATT[CT]
AflIII 995 ACGTGT A^C[GA][CT]GT
ApaI 1325 GGGCCC GGGCC^C
ApoI 1285 GAATTT [GA]^AATT[CT]
Asp718I 1247 GGTACC G^GTACC
AvrII 330 CCTAGG C^CTAGG
BanI 1247 GGTACC G^G[CT][GA]CC
BfaI 331 CTAG C^TAG
BglI 1151 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 330 CCTAGG C^CTAGG
BlpI 432 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC
BsaWI 595 TCCGGT [AT]^CCGG[AT]
BsmBI 468 CGTCTC CGTCTC(1/5)
BsmI 397 GCATTC GAATGC(1/-1)
Bsp1407I 812 TGTACA T^GTACA
BsrGI 812 TGTACA T^GTACA
BssSI 883 CTCGTG CACGAG(-5/-1)
BstAPI 1003 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1269 CGCG CG^CG
BstXI 734 CCACGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 432 GCTCAGC GC^T[ACGT]AGC
DraI 271 TTTAAA TTT^AAA
DraII 1324 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1103 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 519 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1324 GGGGCCC [GA]G^G[ACGT]CC[CT]
KpnI 1247 GGTACC GGTAC^C
MaeI 331 CTAG C^TAG
MvnI 1269 CGCG CG^CG
NlaIII 1278 CATG CATG^
PflMI 750 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 880 GAGCTC GAGCT^C
SapI 183 GCTCTTC GCTCTTC(1/4)
SexAI 496 ACCTGGT A^CC[AT]GGT
SmaI 1328 CCCGGG CCC^GGG
SspBI 812 TGTACA T^GTACA
Tsp509I 1286 AATT ^AATT
Van91I 750 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 734 CCACGGCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1328 CCCGGG C^CCGGG
XmaI 1328 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 684 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
855 GAAGACCTTC
BbsI 855 GAAGAC GAAGAC(2/6)
970 GAAGAC
BglII 752 AGATCT A^GATCT
1224 AGATCT
BpuAI 855 GAAGAC GAAGAC(2/6)
970 GAAGAC
BsaI 493 GAGACC GGTCTC(1/5)
1023 GGTCTC
BsrBI 703 GAGCGG CCGCTC(-3/-3)
1060 GAGCGG
EaeI 1178 CGGCCA [CT]^GGCC[GA]
1271 CGGCCA
EcoT14I 330 CCTAGG C^C[AT][AT]GG
1030 CCAAGG
FauI 705 GCGGG CCCGC(4/6)
1012 GCGGG
HgaI 381 GACGC GACGC(5/10)
1122 GACGC
MaeII 844 ACGT A^CGT
995 ACGT
MseI 272 TTAA T^TAA
1217 TTAA
MslI 735 CACGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1126 CACAAATATG
SfaNI 230 GCATC GCATC(5/9)
1310 GCATC
SmlI 1171 CTCAAG C^T[CT][GA]AG
1228 CTCAAG
StyI 330 CCTAGG C^C[AT][AT]GG
1030 CCAAGG
Tru9I 272 TTAA T^TAA
1217 TTAA
XmnI 684 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
855 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DrdI DsaI EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfcI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII