Restriction map for
pFN21AE1551
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BcnI BfaI BfrI
BglII BlnI BpmI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HapII HgaI Hin1I HindIII
HpaI HpaII KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcsI 151 GAATTT [GA]^AATT[CT]
AhdI 366 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 3 GATCC GGATC(4/5)
AlwNI 644 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 334 CCTGAGG CC^T[ACGT]AGG
ApoI 151 GAATTT [GA]^AATT[CT]
AspEI 366 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 85 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 135 TGGCCA TGG^CCA
BbvCI 544 GCTGAGG CCTCAGC(-5/-2)
BbvI 316 GCTGC GCAGC(8/12)
BclI 498 TGATCA T^GATCA
BglI 680 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 438 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 438 GCTGAGC GC^T[ACGT]AGC
BsaAI 175 TACGTG [CT]AC^GT[GA]
BsaI 475 GAGACC GGTCTC(1/5)
BsiYI 58 CCCTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 58 CCCTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 128 GTCCC GGGAC(10/14)
BsrDI 443 GCAATG GCAATG(2/0)
BssSI 682 CTCGTG CACGAG(-5/-1)
Bsu36I 334 CCTGAGG CC^T[ACGT]AGG
Cac8I 722 GCCTGC GC[ACGT]^[ACGT]GC
CelII 438 GCTGAGC GC^T[ACGT]AGC
CfoI 659 GCGC GCG^C
EaeI 135 TGGCCA [CT]^GGCC[GA]
Eam1105I 366 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 593 CTCTTC CTCTTC(1/4)
Eco81I 334 CCTGAGG CC^T[ACGT]AGG
EcoT14I 750 CCAAGG C^C[AT][AT]GG
FauI 240 CCCGC CCCGC(4/6)
FbaI 498 TGATCA T^GATCA
Fnu4HI 316 GCTGC GC^[ACGT]GC
HhaI 659 GCGC GCG^C
HinP1I 659 GCGC G^CGC
HincII 424 GTCAAC GT[CT]^[GA]AC
HindII 424 GTCAAC GT[CT]^[GA]AC
HinfI 618 GATTC G^A[ACGT]TC
ItaI 316 GCTGC GC^[ACGT]GC
Ksp632I 593 CTCTTC CTCTTC(1/4)
MluNI 135 TGGCCA TGG^CCA
MscI 135 TGGCCA TGG^CCA
MseI 296 TTAA T^TAA
MspA1I 314 CCGCTG C[AC]G^C[GT]G
MwoI 680 GCCTCGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI 85 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 648 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 637 GAGCTC GAGCT^C
SfaNI 21 GATGC GCATC(5/9)
StuI 720 AGGCCT AGG^CCT
StyI 750 CCAAGG C^C[AT][AT]GG
TfiI 618 GATTC G^A[AT]TC
Tru9I 296 TTAA T^TAA
TseI 316 GCTGC G^C[AT]GC
Tth111I 85 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 147 CCATGAATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 241 CCGC CCGC(-3/-1)
314 CCGC
AfaI 114 GTAC GT^AC
141 GTAC
ApaI 29 GGGCCC GGGCC^C
754 GGGCCC
AspHI 571 GAGCAC G[AT]GC[AT]^C
637 GAGCTC
AvaII 127 GGTCC G^G[AT]CC
180 GGACC
BbsI 70 GAAGAC GAAGAC(2/6)
706 GTCTTC
BpuAI 70 GAAGAC GAAGAC(2/6)
706 GTCTTC
BseRI 532 CTCCTC GAGGAG(10/8)
547 GAGGAG
BsiHKAI 571 GAGCAC G[AT]GC[AT]^C
637 GAGCTC
BstF5I 384 CATCC GGATG(2/0)
714 CATCC
FokI 384 CATCC GGATG(9/13)
714 CATCC
HphI 67 GGTGA GGTGA(8/7)
527 TCACC
MaeII 89 ACGT A^CGT
176 ACGT
MslI 45 CACCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
690 CATTGTGGTG
NlaIII 148 CATG CATG^
434 CATG
RsaI 114 GTAC GT^AC
141 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BanII BmyI BsaJI BsmAI
Bsp1286I BsrI BssKI BstNI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
HaeIII MaeIII MboI MboII MnlI
MvaI NdeII NlaIV Sau3AI Sau96I
ScrFI Tsp45I Tsp509I TspRI