Restriction map for
pFN21AE4454
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbsI BciVI
BclI BfrI BglI BglII BlnI
BpuAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 994 CGCG CG^CG
Alw44I 484 GTGCAC G^TGCAC
AlwNI 1141 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 973 CCTGAGG CC^T[ACGT]AGG
ApaI 74 GGGCCC GGGCC^C
ApaLI 484 GTGCAC G^TGCAC
AvaI 1250 CCCGAG C^[CT]CG[GA]G
BbrPI 1330 CACGTG CAC^GTG
BlpI 930 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 930 GCTAAGC GC^T[ACGT]AGC
BsaAI 1330 CACGTG [CT]AC^GT[GA]
BsaBI 426 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 737 GGTCTC GGTCTC(1/5)
BsmBI 304 CGTCTC CGTCTC(1/5)
BsoBI 1250 CCCGAG C^[CT]CG[GA]G
BspMI 218 ACCTGC ACCTGC(4/8)
BsrBI 299 CCGCTC CCGCTC(-3/-3)
BsrFI 1076 GCCGGT [GA]^CCGG[CT]
BssSI 1315 CTCGTG CACGAG(-5/-1)
BstBI 1346 TTCGAA TT^CGAA
BstUI 994 CGCG CG^CG
BstYI 533 AGATCC [GA]^GATC[CT]
Bsu36I 973 CCTGAGG CC^T[ACGT]AGG
CelII 930 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1076 GCCGGT [GA]^CCGG[CT]
DsaI 65 CCGTGG C^C[GA][CT]GG
EarI 154 CTCTTC CTCTTC(1/4)
Eco81I 973 CCTGAGG CC^T[ACGT]AGG
EcoRV 721 GATATC GAT^ATC
HinfI 369 GAATC G^A[ACGT]TC
Ksp632I 154 CTCTTC CTCTTC(1/4)
MamI 426 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 533 AGATCC [GA]^GATC[CT]
MvnI 994 CGCG CG^CG
NspV 1346 TTCGAA TT^CGAA
PmaCI 1330 CACGTG CAC^GTG
PmlI 1330 CACGTG CAC^GTG
PpuMI 100 AGGACCT [GA]G^G[AT]CC[CT]
SacI 589 GAGCTC GAGCT^C
SapI 153 GCTCTTC GCTCTTC(1/4)
SfcI 829 CTACAG C^T[GA][CT]AG
SfuI 1346 TTCGAA TT^CGAA
SmlI 629 CTCAAG C^T[CT][GA]AG
TfiI 369 GAATC G^A[AT]TC
Tsp45I 864 GTCAC ^GT[GC]AC
XhoII 533 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 827 GTCTAC GT^[AC][GT]AC
848 GTCTAC
AfaI 822 GTAC GT^AC
1138 GTAC
AflIII 383 ACATGT A^C[GA][CT]GT
492 ACATGT
AlwI 534 GATCC GGATC(4/5)
702 GATCC
AspHI 484 GTGCAC G[AT]GC[AT]^C
589 GAGCTC
BbvCI 82 GCTGAGG CCTCAGC(-5/-2)
96 GCTGAGG
BcgI 1056 CGAGGCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1338 GCATCTACTTCG
BseRI 46 GAGGAG GAGGAG(10/8)
1168 GAGGAG
BsgI 202 GTGCAG GTGCAG(16/14)
919 GTGCAG
BsiHKAI 484 GTGCAC G[AT]GC[AT]^C
589 GAGCTC
BsmAI 305 GTCTC GTCTC(1/5)
738 GTCTC
BspLU11I 383 ACATGT A^CATGT
492 ACATGT
BsrDI 342 CATTGC GCAATG(2/0)
888 CATTGC
FauI 986 CCCGC CCCGC(4/6)
1387 GCGGG
MaeII 846 ACGT A^CGT
1331 ACGT
MaeIII 864 GTCAC ^GT[ACGT]AC
1112 GTAAC
PflMI 5 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
578 CCAAAGCCTGG
RsaI 822 GTAC GT^AC
1138 GTAC
SphI 944 GCATGC GCATG^C
1365 GCATGC
Van91I 5 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
578 CCAAAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AvaII BalI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeI
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PvuII Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp509I TspRI TthHB8I
XcmI