Restriction map for
pFN21AE1370
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvrII BamHI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI HgaI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MseI MspA1I MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 90 CGCG CG^CG
AcsI 718 GAATTC [GA]^AATT[CT]
AcyI 3 GGCGCC G[GA]^CG[CT]C
AlwI 169 GATCC GGATC(4/5)
AlwNI 58 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 718 GAATTC [GA]^AATT[CT]
Asp700I 15 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 276 GGTCC G^G[AT]CC
AviII 189 TGCGCA TGC^GCA
BalI 662 TGGCCA TGG^CCA
BanII 70 GAGCTC G[GA]GC[CT]^C
BbeI 3 GGCGCC GGCGC^C
BbrPI 267 CACGTG CAC^GTG
BbvI 187 GCTGC GCAGC(8/12)
BclI 248 TGATCA T^GATCA
BcnI 155 CCCGG CC^[GC]GG
BsaAI 267 CACGTG [CT]AC^GT[GA]
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsiEI 104 CGGCCG CG[GA][CT]^CG
BsmAI 589 GTCTC GTCTC(1/5)
BsmBI 588 CGTCTC CGTCTC(1/5)
BsmFI 360 GGGAC GGGAC(10/14)
BspMI 649 ACCTGC ACCTGC(4/8)
BsrFI 102 GCCGGC [GA]^CCGG[CT]
BssSI 556 CACGAG CACGAG(-5/-1)
BstUI 90 CGCG CG^CG
BstXI 266 CCACGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 168 AGATCC [GA]^GATC[CT]
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
EagI 104 CGGCCG C^GGCCG
EclXI 104 CGGCCG C^GGCCG
Eco52I 104 CGGCCG C^GGCCG
EcoRI 718 GAATTC G^AATTC
EcoT14I 95 CCAAGG C^C[AT][AT]GG
FbaI 248 TGATCA T^GATCA
FspI 189 TGCGCA TGC^GCA
HaeII 3 GGCGCC [GA]GCGC^[CT]
Hin1I 3 GGCGCC G[GA]^CG[CT]C
KasI 3 GGCGCC G^GCGCC
MflI 168 AGATCC [GA]^GATC[CT]
MluNI 662 TGGCCA TGG^CCA
MscI 662 TGGCCA TGG^CCA
MvnI 90 CGCG CG^CG
NaeI 102 GCCGGC GCC^GGC
NarI 3 GGCGCC GG^CGCC
NciI 155 CCCGG CC^[GC]GG
NgoMIV 102 GCCGGC G^CCGGC
NspI 300 GCATGT [GA]CATG^[CT]
PflMI 345 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 267 CACGTG CAC^GTG
PmlI 267 CACGTG CAC^GTG
PshAI 441 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 70 GAGCTC GAGCT^C
SfoI 3 GGCGCC GGC^GCC
SmlI 709 CTCAAG C^T[CT][GA]AG
StyI 95 CCAAGG C^C[AT][AT]GG
TseI 187 GCTGC G^C[AT]GC
Tsp509I 719 AATT ^AATT
TspRI 417 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 345 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 173 CCAAGATTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 168 AGATCC [GA]^GATC[CT]
XmnI 15 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 515 GTAC GT^AC
553 GTAC
BanI 3 GGCGCC G^G[CT][GA]CC
99 GGTGCC
BfaI 166 CTAG C^TAG
394 CTAG
BpmI 9 CTCCAG CTGGAG(16/14)
471 CTGGAG
BsiYI 345 CCATGCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
377 CCTGCATTGGG
BslI 345 CCATGCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
377 CCTGCATTGGG
BstF5I 571 GGATG GGATG(2/0)
681 GGATG
CfoI 4 GCGC GCG^C
190 GCGC
Fnu4HI 91 GCGGC GC^[ACGT]GC
187 GCTGC
FokI 571 GGATG GGATG(9/13)
681 GGATG
HapII 103 CCGG C^CGG
156 CCGG
HhaI 4 GCGC GCG^C
190 GCGC
HinP1I 4 GCGC G^CGC
190 GCGC
HpaII 103 CCGG C^CGG
156 CCGG
ItaI 91 GCGGC GC^[ACGT]GC
187 GCTGC
MaeI 166 CTAG C^TAG
394 CTAG
MaeIII 208 GTGAC ^GT[ACGT]AC
637 GTGAC
MboII 39 GAAGA GAAGA(8/7)
20 TCTTC
MslI 111 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
267 CACGTGGGTG
MspI 103 CCGG C^CGG
156 CCGG
MwoI 181 GCTGTGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
324 GCTGATTGGGC
RsaI 515 GTAC GT^AC
553 GTAC
SfaNI 595 GATGC GCATC(5/9)
413 GCATC
TaqI 146 TCGA T^CGA
434 TCGA
Tsp45I 208 GTGAC ^GT[GC]AC
637 GTGAC
TthHB8I 146 TCGA T^CGA
434 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI AspHI BmyI BsaJI
BsiHKAI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII HaeIII HinfI MaeII MboI
MnlI MvaI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI