Restriction map for
pFN21AE6142
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BpmI
BsaAI BsaBI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstF5I
BstXI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NaeI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 135 CGCG CG^CG
AcyI 79 GGCGCC G[GA]^CG[CT]C
AfaI 171 GTAC GT^AC
AspHI 193 GAGCAC G[AT]GC[AT]^C
BanI 79 GGCGCC G^G[CT][GA]CC
BanII 96 GGGCTC G[GA]GC[CT]^C
BbeI 79 GGCGCC GGCGC^C
BbsI 114 GAAGAC GAAGAC(2/6)
BbvI 33 GCTGC GCAGC(8/12)
BlpI 98 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 98 GCTCAGC GC^T[ACGT]AGC
BpuAI 114 GAAGAC GAAGAC(2/6)
BsaHI 79 GGCGCC G[GA]^CG[CT]C
BsaI 201 GAGACC GGTCTC(1/5)
BsiHKAI 193 GAGCAC G[AT]GC[AT]^C
BsmAI 201 GAGAC GTCTC(1/5)
BsmFI 45 GTCCC GGGAC(10/14)
BsrI 310 ACTGG ACTGG(1/-1)
BssSI 196 CACGAG CACGAG(-5/-1)
BstEII 265 GGTAACC G^GT[ACGT]ACC
BstNI 254 CCTGG CC^[AT]GG
BstPI 265 GGTAACC G^GT[ACGT]ACC
BstUI 135 CGCG CG^CG
CelII 98 GCTCAGC GC^T[ACGT]AGC
DdeI 99 CTCAG C^T[ACGT]AG
EarI 91 GAAGAG CTCTTC(1/4)
EcoO65I 265 GGTAACC G^GT[ACGT]ACC
EcoRII 254 CCTGG ^CC[AT]GG
EcoT14I 84 CCATGG C^C[AT][AT]GG
FauI 47 CCCGC CCCGC(4/6)
HaeII 79 GGCGCC [GA]GCGC^[CT]
HaeIII 65 GGCC GG^CC
HapII 143 CCGG C^CGG
Hin1I 79 GGCGCC G[GA]^CG[CT]C
HpaII 143 CCGG C^CGG
HphI 122 TCACC GGTGA(8/7)
KasI 79 GGCGCC G^GCGCC
Ksp632I 91 GAAGAG CTCTTC(1/4)
MaeIII 266 GTAAC ^GT[ACGT]AC
MspA1I 55 CAGCTG C[AC]G^C[GT]G
MspI 143 CCGG C^CGG
MvaI 254 CCTGG CC^[AT]GG
MvnI 135 CGCG CG^CG
NarI 79 GGCGCC GG^CGCC
NcoI 84 CCATGG C^CATGG
PleI 117 GACTC GAGTC(4/5)
PvuII 55 CAGCTG CAG^CTG
RsaI 171 GTAC GT^AC
SfaNI 233 GCATC GCATC(5/9)
SfoI 79 GGCGCC GGC^GCC
SmaI 142 CCCGGG CCC^GGG
SphI 158 GCATGC GCATG^C
StyI 84 CCATGG C^C[AT][AT]GG
TaqI 73 TCGA T^CGA
TfiI 297 GAATC G^A[AT]TC
TseI 33 GCTGC G^C[AT]GC
TthHB8I 73 TCGA T^CGA
XmaCI 142 CCCGGG C^CCGGG
XmaI 142 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 56 AGCT AG^CT
102 AGCT
AvaI 142 CCCGGG C^[CT]CG[GA]G
205 CCCGAG
AvaII 44 GGTCC G^G[AT]CC
239 GGACC
BcnI 142 CCCGG CC^[GC]GG
143 CCGGG
BmyI 96 GGGCTC G[AGT]GC[ACT]^C
193 GAGCAC
BsoBI 142 CCCGGG C^[CT]CG[GA]G
205 CCCGAG
Bsp1286I 96 GGGCTC G[AGT]GC[ACT]^C
193 GAGCAC
Cac8I 103 GCTCGC GC[ACGT]^[ACGT]GC
158 GCATGC
CfoI 80 GCGC GCG^C
136 GCGC
Cfr13I 44 GGTCC G^G[ACGT]CC
239 GGACC
DsaI 84 CCATGG C^C[GA][CT]GG
186 CCACGG
Fnu4HI 33 GCTGC GC^[ACGT]GC
63 GCGGC
HhaI 80 GCGC GCG^C
136 GCGC
HinP1I 80 GCGC G^CGC
136 GCGC
HinfI 117 GACTC G^A[ACGT]TC
297 GAATC
ItaI 33 GCTGC GC^[ACGT]GC
63 GCGGC
NciI 142 CCCGG CC^[GC]GG
143 CCGGG
NlaIV 44 GGTCCC GG[ACGT]^[ACGT]CC
79 GGCGCC
NspI 107 GCATGT [GA]CATG^[CT]
158 GCATGC
Sau96I 44 GGTCC G^G[ACGT]CC
239 GGACC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AciI BsaJI BsiYI BslI BssKI
MboII MnlI MwoI NlaIII ScrFI