Restriction map for
pFN21AE1485
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaII AviII AvrII BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstXI CelII CfoI Cfr13I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI HphI
KasI KpnI KspI MamI MluI
MroI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
Sau96I SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI StuI Tsp45I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 63 CGCG CG^CG
AocI 633 CCTGAGG CC^T[ACGT]AGG
Asp700I 1015 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 325 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 338 CCCGAG C^[CT]CG[GA]G
BamHI 448 GGATCC G^GATCC
BbsI 58 GTCTTC GAAGAC(2/6)
BglII 297 AGATCT A^GATCT
BmyI 1167 GGGCAC G[AGT]GC[ACT]^C
BpmI 1097 CTGGAG CTGGAG(16/14)
BpuAI 58 GTCTTC GAAGAC(2/6)
BsaJI 1068 CCATGG C^C[ACGT][ACGT]GG
BsmFI 1106 GGGAC GGGAC(10/14)
BsmI 927 GCATTC GAATGC(1/-1)
BsoBI 338 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1167 GGGCAC G[AGT]GC[ACT]^C
BsrBI 258 GAGCGG CCGCTC(-3/-3)
BsrDI 745 CATTGC GCAATG(2/0)
Bst1107I 1056 GTATAC GTA^TAC
BstAPI 1018 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 63 CGCG CG^CG
BstZ17I 1056 GTATAC GTA^TAC
Bsu36I 633 CCTGAGG CC^T[ACGT]AGG
DsaI 1068 CCATGG C^C[GA][CT]GG
Eco81I 633 CCTGAGG CC^T[ACGT]AGG
EcoRI 263 GAATTC G^AATTC
EcoT14I 1068 CCATGG C^C[AT][AT]GG
HincII 229 GTCGAC GT[CT]^[GA]AC
HindII 229 GTCGAC GT[CT]^[GA]AC
MaeII 290 ACGT A^CGT
MfeI 1148 CAATTG C^AATTG
MunI 1148 CAATTG C^AATTG
MvnI 63 CGCG CG^CG
NcoI 1068 CCATGG C^CATGG
NlaIV 448 GGATCC GG[ACGT]^[ACGT]CC
PflFI 325 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 850 GAGTC GAGTC(4/5)
PvuII 173 CAGCTG CAG^CTG
SalI 229 GTCGAC G^TCGAC
SapI 91 GCTCTTC GCTCTTC(1/4)
SexAI 1051 ACCAGGT A^CC[AT]GGT
SmlI 885 CTTGAG C^T[CT][GA]AG
SpeI 1039 ACTAGT A^CTAGT
SphI 923 GCATGC GCATG^C
SspI 1136 AATATT AAT^ATT
StyI 1068 CCATGG C^C[AT][AT]GG
SwaI 248 ATTTAAAT ATTT^AAAT
Tth111I 325 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1015 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 229 GTCGAC GT^[AC][GT]AC
1056 GTATAC
AcsI 263 GAATTC [GA]^AATT[CT]
400 GAATTT
ApoI 263 GAATTC [GA]^AATT[CT]
400 GAATTT
BalI 75 TGGCCA TGG^CCA
1065 TGGCCA
BsmAI 999 GTCTC GTCTC(1/5)
1234 GTCTC
BsrFI 165 GCCGGT [GA]^CCGG[CT]
660 GCCGGT
BssKI 1052 CCAGG ^CC[ACGT]GG
1096 CCTGG
BstNI 1052 CCAGG CC^[AT]GG
1096 CCTGG
Cac8I 282 GCTGGC GC[ACGT]^[ACGT]GC
923 GCATGC
Cfr10I 165 GCCGGT [GA]^CCGG[CT]
660 GCCGGT
DraI 249 TTTAAA TTT^AAA
480 TTTAAA
EaeI 75 TGGCCA [CT]^GGCC[GA]
1065 TGGCCA
EarI 40 CTCTTC CTCTTC(1/4)
92 CTCTTC
EcoRII 1052 CCAGG ^CC[AT]GG
1096 CCTGG
EcoT22I 218 ATGCAT ATGCA^T
925 ATGCAT
HindIII 4 AAGCTT A^AGCTT
1220 AAGCTT
Ksp632I 40 CTCTTC CTCTTC(1/4)
92 CTCTTC
MaeIII 169 GTAAC ^GT[ACGT]AC
1196 GTTAC
MluNI 75 TGGCCA TGG^CCA
1065 TGGCCA
MscI 75 TGGCCA TGG^CCA
1065 TGGCCA
MspA1I 173 CAGCTG C[AC]G^C[GT]G
1229 CCGCTG
MvaI 1052 CCAGG CC^[AT]GG
1096 CCTGG
NdeI 143 CATATG CA^TATG
519 CATATG
NsiI 218 ATGCAT ATGCA^T
925 ATGCAT
PflMI 545 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
782 CCAAACACTGG
ScrFI 1052 CCAGG CC^[ACGT]GG
1096 CCTGG
SfcI 124 CTACAG C^T[GA][CT]AG
1212 CTGTAG
Van91I 545 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
782 CCAAACACTGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BsiYI BslI BsrI BstF5I
BstYI DdeI DpnII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MboI MboII MflI MnlI
MseI MslI MspI MwoI NdeII
NlaIII NspI RsaI Sau3AI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII