Restriction map for
pFN21AE6140
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# NO CUT ENZYMES ( 165 enzymes) #
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Acc65I AccI AccII AclI AcsI
AfaI AflII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspHI AspI AvaI AviII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlpI
BpmI Bpu1102I BsaAI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstF5I
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MfeI MflI MluI
MluNI MscI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 402 GACGTC GACGT^C
AccIII 491 TCCGGA T^CCGGA
AflIII 474 ACACGT A^C[GA][CT]GT
AhdI 397 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 397 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 231 CCTAGG C^CTAGG
BanI 346 GGCACC G^G[CT][GA]CC
BanII 181 GAGCCC G[GA]GC[CT]^C
BbsI 280 GAAGAC GAAGAC(2/6)
BfaI 232 CTAG C^TAG
BglI 97 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 231 CCTAGG C^CTAGG
BpuAI 280 GAAGAC GAAGAC(2/6)
BsaBI 501 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 491 TCCGGA [AT]^CCGG[AT]
BseAI 491 TCCGGA T^CCGGA
BsmBI 236 GAGACG CGTCTC(1/5)
BspEI 491 TCCGGA T^CCGGA
BspHI 353 TCATGA T^CATGA
BsrBI 29 GAGCGG CCGCTC(-3/-3)
BstEII 408 GGTGACC G^GT[ACGT]ACC
BstPI 408 GGTGACC G^GT[ACGT]ACC
DpnII 501 GATC ^GATC
DsaI 119 CCATGG C^C[GA][CT]GG
Eam1105I 397 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 408 GGTGACC G^GT[ACGT]ACC
MaeI 232 CTAG C^TAG
MamI 501 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 501 GATC ^GATC
MroI 491 TCCGGA T^CCGGA
MspA1I 379 CAGCTG C[AC]G^C[GT]G
NcoI 119 CCATGG C^CATGG
NdeII 501 GATC ^GATC
NspI 137 GCATGT [GA]CATG^[CT]
PpuMI 110 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 379 CAGCTG CAG^CTG
RcaI 353 TCATGA T^CATGA
Sau3AI 501 GATC ^GATC
TaqI 314 TCGA T^CGA
TthHB8I 314 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Asp700I 11 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
357 GAACACCTTC
AvaII 111 GGACC G^G[AT]CC
396 GGACC
BsmAI 236 GAGAC GTCTC(1/5)
266 GAGAC
BstNI 108 CCAGG CC^[AT]GG
186 CCTGG
DdeI 133 CTGAG C^T[ACGT]AG
326 CTGAG
DraII 110 AGGACCC [GA]G^G[ACGT]CC[CT]
128 AGGCCCT
DrdI 283 GACATCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
343 GACGGCACCGTC
EcoO109I 110 AGGACCC [GA]G^G[ACGT]CC[CT]
128 AGGCCCT
EcoRII 108 CCAGG ^CC[AT]GG
186 CCTGG
EcoT14I 119 CCATGG C^C[AT][AT]GG
231 CCTAGG
HgaI 238 GACGC GACGC(5/10)
456 GCGTC
MslI 312 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
348 CACCGTCATG
MvaI 108 CCAGG CC^[AT]GG
186 CCTGG
StyI 119 CCATGG C^C[AT][AT]GG
231 CCTAGG
XmnI 11 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
357 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcyI AluI BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI Cac8I Cfr13I
EarI FauI Fnu4HI HaeIII HapII
Hin1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboII MnlI MspI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI TseI Tsp45I