Restriction map for
pFN21AE5403
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaI BseAI BsiWI
BsmFI BsmI BspDI BspEI BspLU11I
BsrBI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI KpnI MaeII MamI
MfeI MluI MroI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SalI SapI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI Tru9I Tsp45I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AcsI 585 GAATTT [GA]^AATT[CT]
AcyI 227 GGCGCC G[GA]^CG[CT]C
AgeI 284 ACCGGT A^CCGGT
Alw44I 337 GTGCAC G^TGCAC
AlwNI 76 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 141 CCTGAGG CC^T[ACGT]AGG
Aor51HI 243 AGCGCT AGC^GCT
ApaI 15 GGGCCC GGGCC^C
ApaLI 337 GTGCAC G^TGCAC
ApoI 585 GAATTT [GA]^AATT[CT]
BalI 368 TGGCCA TGG^CCA
BanI 227 GGCGCC G^G[CT][GA]CC
BbeI 227 GGCGCC GGCGC^C
BbsI 393 GAAGAC GAAGAC(2/6)
BbvCI 316 GCTGAGG CCTCAGC(-5/-2)
BlpI 36 GCTCAGC GC^T[ACGT]AGC
BpmI 433 CTCCAG CTGGAG(16/14)
Bpu1102I 36 GCTCAGC GC^T[ACGT]AGC
BpuAI 393 GAAGAC GAAGAC(2/6)
BsaHI 227 GGCGCC G[GA]^CG[CT]C
BsaWI 284 ACCGGT [AT]^CCGG[AT]
BseRI 184 GAGGAG GAGGAG(10/8)
BsmAI 259 GAGAC GTCTC(1/5)
BsmBI 259 GAGACG CGTCTC(1/5)
BspHI 389 TCATGA T^CATGA
BspMI 942 ACCTGC ACCTGC(4/8)
BsrDI 499 GCAATG GCAATG(2/0)
BsrFI 284 ACCGGT [GA]^CCGG[CT]
BsrI 902 ACTGG ACTGG(1/-1)
BstBI 686 TTCGAA TT^CGAA
BstYI 219 GGATCT [GA]^GATC[CT]
Bsu36I 141 CCTGAGG CC^T[ACGT]AGG
CelII 36 GCTCAGC GC^T[ACGT]AGC
Cfr10I 284 ACCGGT [GA]^CCGG[CT]
DraII 204 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 116 CCGCGG C^C[GA][CT]GG
EarI 232 CTCTTC CTCTTC(1/4)
Eco47III 243 AGCGCT AGC^GCT
Eco81I 141 CCTGAGG CC^T[ACGT]AGG
EcoO109I 204 GGGGCCT [GA]G^G[ACGT]CC[CT]
Hin1I 227 GGCGCC G[GA]^CG[CT]C
HincII 610 GTTGAC GT[CT]^[GA]AC
HindII 610 GTTGAC GT[CT]^[GA]AC
KasI 227 GGCGCC G^GCGCC
Ksp632I 232 CTCTTC CTCTTC(1/4)
KspI 116 CCGCGG CCGC^GG
MaeIII 559 GTTAC ^GT[ACGT]AC
MflI 219 GGATCT [GA]^GATC[CT]
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
NarI 227 GGCGCC GG^CGCC
NspV 686 TTCGAA TT^CGAA
PflMI 495 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 284 ACCGGT A^CCGGT
PvuII 63 CAGCTG CAG^CTG
RcaI 389 TCATGA T^CATGA
SacI 34 GAGCTC GAGCT^C
SacII 116 CCGCGG CCGC^GG
SfcI 728 CTATAG C^T[GA][CT]AG
SfoI 227 GGCGCC GGC^GCC
SfuI 686 TTCGAA TT^CGAA
SmlI 593 CTCAAG C^T[CT][GA]AG
SspI 984 AATATT AAT^ATT
Van91I 495 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 219 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 794 GTAC GT^AC
817 GTAC
AvaI 18 CCCGAG C^[CT]CG[GA]G
256 CCCGAG
BbvI 90 GCAGC GCAGC(8/12)
29 GCTGC
BciVI 516 GGATAC GTATCC(6/5)
570 GTATCC
BcnI 13 CCGGG CC^[GC]GG
71 CCGGG
BfaI 524 CTAG C^TAG
695 CTAG
BsaJI 116 CCGCGG C^C[ACGT][ACGT]GG
208 CCTGGG
BsgI 88 GTGCAG GTGCAG(16/14)
669 GTGCAG
BsiEI 119 CGGCCG CG[GA][CT]^CG
161 CGGCCG
BsoBI 18 CCCGAG C^[CT]CG[GA]G
256 CCCGAG
Bsp1407I 793 TGTACA T^GTACA
816 TGTACA
BsrGI 793 TGTACA T^GTACA
816 TGTACA
EagI 119 CGGCCG C^GGCCG
161 CGGCCG
EclXI 119 CGGCCG C^GGCCG
161 CGGCCG
Eco52I 119 CGGCCG C^GGCCG
161 CGGCCG
MaeI 524 CTAG C^TAG
695 CTAG
MslI 159 CACGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
340 CACTGTCATG
MspA1I 63 CAGCTG C[AC]G^C[GT]G
116 CCGCGG
NciI 13 CCGGG CC^[GC]GG
71 CCGGG
RsaI 794 GTAC GT^AC
817 GTAC
SfaNI 78 GATGC GCATC(5/9)
155 GCATC
SspBI 793 TGTACA T^GTACA
816 TGTACA
TfiI 438 GATTC G^A[AT]TC
829 GAATC
TseI 29 GCTGC G^C[AT]GC
90 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BanII BmyI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MnlI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI TaqI
Tsp509I TspRI TthHB8I