Restriction map for
pFN21AE1442
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# NO CUT ENZYMES ( 168 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HaeIII HgaI HindIII HpaI HphI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp509I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 582 GACGTC GACGT^C
AcyI 582 GACGTC G[GA]^CG[CT]C
AlwNI 135 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 463 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 75 GGATCC G^GATCC
BcgI 587 CGACTGCGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 499 CCGGG CC^[GC]GG
BsaHI 582 GACGTC G[GA]^CG[CT]C
BsaI 687 GAGACC GGTCTC(1/5)
BsgI 261 CTGCAC GTGCAG(16/14)
BsiYI 492 CCTCTCTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 492 CCTCTCTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 687 GAGAC GTCTC(1/5)
BspMI 166 GCAGGT ACCTGC(4/8)
BsrI 691 CCAGT ACTGG(1/-1)
BstYI 75 GGATCC [GA]^GATC[CT]
DraII 178 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 598 CTCTTC CTCTTC(1/4)
EcoO109I 178 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 519 CCCGC CCCGC(4/6)
HapII 499 CCGG C^CGG
Hin1I 582 GACGTC G[GA]^CG[CT]C
HinfI 123 GAATC G^A[ACGT]TC
HpaII 499 CCGG C^CGG
Ksp632I 598 CTCTTC CTCTTC(1/4)
MflI 75 GGATCC [GA]^GATC[CT]
MspI 499 CCGG C^CGG
NciI 499 CCGGG CC^[GC]GG
NdeI 189 CATATG CA^TATG
NlaIII 300 CATG CATG^
NlaIV 75 GGATCC GG[ACGT]^[ACGT]CC
NspI 299 GCATGT [GA]CATG^[CT]
PflFI 463 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 178 AGGACCT [GA]G^G[AT]CC[CT]
SapI 597 GCTCTTC GCTCTTC(1/4)
SfcI 720 CTGTAG C^T[GA][CT]AG
SmlI 704 CTCAAG C^T[CT][GA]AG
TfiI 123 GAATC G^A[AT]TC
Tth111I 463 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 29 CCACTCTGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 75 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 398 GTCGAC GT^[AC][GT]AC
585 GTCGAC
AciI 520 CCGC CCGC(-3/-1)
553 CCGC
AfaI 101 GTAC GT^AC
694 GTAC
AlwI 76 GATCC GGATC(4/5)
75 GGATC
BbvI 296 GCAGC GCAGC(8/12)
725 GCTGC
BsrDI 68 CATTGC GCAATG(2/0)
245 GCAATG
Cac8I 194 GCAGGC GC[ACGT]^[ACGT]GC
522 GCCTGC
CfoI 164 GCGC GCG^C
577 GCGC
Fnu4HI 296 GCAGC GC^[ACGT]GC
725 GCTGC
HhaI 164 GCGC GCG^C
577 GCGC
HinP1I 164 GCGC G^CGC
577 GCGC
HincII 398 GTCGAC GT[CT]^[GA]AC
585 GTCGAC
HindII 398 GTCGAC GT[CT]^[GA]AC
585 GTCGAC
ItaI 296 GCAGC GC^[ACGT]GC
725 GCTGC
MseI 374 TTAA T^TAA
638 TTAA
MspA1I 9 CAGCTG C[AC]G^C[GT]G
95 CAGCTG
MwoI 597 GCTCTTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
719 GCTGTAGCTGC
PvuII 9 CAGCTG CAG^CTG
95 CAGCTG
RsaI 101 GTAC GT^AC
694 GTAC
SalI 398 GTCGAC G^TCGAC
585 GTCGAC
TaqI 399 TCGA T^CGA
586 TCGA
Tru9I 374 TTAA T^TAA
638 TTAA
TseI 296 GCAGC G^C[AT]GC
725 GCTGC
TspRI 199 CAGTG CA[GC]TG[ACGT][ACGT]^
277 CACTG
TthHB8I 399 TCGA T^CGA
586 TCGA
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AvaII BpmI BsaJI BsmFI
BssKI BstF5I BstNI Cfr13I DpnII
EcoRII FokI MaeII MaeIII MboI
MboII MnlI MvaI NdeII Sau3AI
Sau96I ScrFI SfaNI Tsp45I