##################################
# NO CUT ENZYMES (  225 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DdeI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        ItaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       TseI        Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AcsI          276  GAATTT               [GA]^AATT[CT]
AfaI          153  GTAC                 GT^AC
ApoI          276  GAATTT               [GA]^AATT[CT]
AspI           83  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BssKI         103  CCTGG                ^CC[ACGT]GG
BstNI         103  CCTGG                CC^[AT]GG
Cac8I         240  GCAAGC               GC[ACGT]^[ACGT]GC
Cfr13I        100  GGCCC                G^G[ACGT]CC
DraII          99  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I       99  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        103  CCTGG                ^CC[AT]GG
EcoT14I        26  CCTTGG               C^C[AT][AT]GG
HaeIII        100  GGCC                 GG^CC
HgaI           83  GACGC                GACGC(5/10)
MvaI          103  CCTGG                CC^[AT]GG
PflFI          83  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsaI          153  GTAC                 GT^AC
Sau96I        100  GGCCC                G^G[ACGT]CC
ScrFI         103  CCTGG                CC^[ACGT]GG
SfaNI         218  GATGC                GCATC(5/9)
StyI           26  CCTTGG               C^C[AT][AT]GG
TaqI          235  TCGA                 T^CGA
Tsp45I        170  GTGAC                ^GT[GC]AC
Tsp509I       277  AATT                 ^AATT
TspRI         116  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I        83  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       235  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

MaeIII        161  GTAAC                ^GT[ACGT]AC
              170  GTGAC               
MnlI          143  CCTC                 CCTC(7/6)
               80  GAGG                
MwoI           86  GCTGTCTTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              240  GCAAGCAAGGC         
NlaIII         15  CATG                 CATG^
               69  CATG                
SfcI           20  CTATAG               C^T[GA][CT]AG
               47  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

BsaJI