Restriction map for
pFN21AE1536
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BmyI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 263 AACGTT AA^CGTT
AcyI 73 GACGCC G[GA]^CG[CT]C
AlwI 130 GATCC GGATC(4/5)
AlwNI 424 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 439 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 32 GGACC G^G[AT]CC
BalI 104 TGGCCA TGG^CCA
BbvI 51 GCAGC GCAGC(8/12)
BcnI 387 CCGGG CC^[GC]GG
BglI 661 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 847 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 847 GCTGAGC GC^T[ACGT]AGC
BsaHI 73 GACGCC G[GA]^CG[CT]C
BsiYI 496 CCATTTGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 496 CCATTTGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 907 GTCTC GTCTC(1/5)
BsmBI 906 CGTCTC CGTCTC(1/5)
BspHI 150 TCATGA T^CATGA
BsrDI 220 GCAATG GCAATG(2/0)
BstF5I 603 GGATG GGATG(2/0)
CelII 847 GCTGAGC GC^T[ACGT]AGC
Cfr13I 32 GGACC G^G[ACGT]CC
DsaI 101 CCGTGG C^C[GA][CT]GG
EaeI 104 TGGCCA [CT]^GGCC[GA]
EarI 876 GAAGAG CTCTTC(1/4)
FokI 603 GGATG GGATG(9/13)
HapII 387 CCGG C^CGG
HgaI 73 GACGC GACGC(5/10)
Hin1I 73 GACGCC G[GA]^CG[CT]C
HpaII 387 CCGG C^CGG
HphI 297 TCACC GGTGA(8/7)
Ksp632I 876 GAAGAG CTCTTC(1/4)
MluNI 104 TGGCCA TGG^CCA
MscI 104 TGGCCA TGG^CCA
MspI 387 CCGG C^CGG
NciI 387 CCGGG CC^[GC]GG
Psp1406I 263 AACGTT AA^CGTT
RcaI 150 TCATGA T^CATGA
Sau96I 32 GGACC G^G[ACGT]CC
SexAI 953 ACCTGGT A^CC[AT]GGT
SmlI 142 CTTGAG C^T[CT][GA]AG
TaqI 515 TCGA T^CGA
TseI 51 GCAGC G^C[AT]GC
Tsp45I 735 GTCAC ^GT[GC]AC
TspRI 28 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 515 TCGA T^CGA
XmnI 439 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 3 GTAC GT^AC
242 GTAC
BglII 229 AGATCT A^GATCT
796 AGATCT
BpmI 39 CTCCAG CTGGAG(16/14)
663 CTCCAG
BseRI 46 GAGGAG GAGGAG(10/8)
928 GAGGAG
BstYI 229 AGATCT [GA]^GATC[CT]
796 AGATCT
EcoRI 196 GAATTC G^AATTC
349 GAATTC
FauI 527 CCCGC CCCGC(4/6)
66 GCGGG
Fnu4HI 24 GCGGC GC^[ACGT]GC
51 GCAGC
HaeIII 105 GGCC GG^CC
333 GGCC
ItaI 24 GCGGC GC^[ACGT]GC
51 GCAGC
MaeIII 735 GTCAC ^GT[ACGT]AC
741 GTTAC
MflI 229 AGATCT [GA]^GATC[CT]
796 AGATCT
MspA1I 52 CAGCTG C[AC]G^C[GT]G
569 CAGCTG
PleI 393 GAGTC GAGTC(4/5)
702 GAGTC
PvuII 52 CAGCTG CAG^CTG
569 CAGCTG
RsaI 3 GTAC GT^AC
242 GTAC
TfiI 546 GAATC G^A[AT]TC
961 GAATC
XhoII 229 AGATCT [GA]^GATC[CT]
796 AGATCT
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcsI AluI ApoI BfaI
BsaJI BsrI BssKI BstNI Cac8I
DdeI DpnII EcoRII HinfI MaeI
MaeII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII Sau3AI
ScrFI SfaNI Tru9I Tsp509I