Restriction map for
pFN21AE6335
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AclI AcsI
AfaI AflII AgeI AhdI Alw44I
ApaI ApaLI ApoI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BanII BbrPI BbvCI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeII MaeIII MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 67 GGCGCC G[GA]^CG[CT]C
AflIII 653 ACATGT A^C[GA][CT]GT
AocI 664 CCTCAGG CC^T[ACGT]AGG
AseI 186 ATTAAT AT^TAAT
AsnI 186 ATTAAT AT^TAAT
Asp700I 18 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 872 CTCGGG C^[CT]CG[GA]G
BalI 726 TGGCCA TGG^CCA
BbeI 67 GGCGCC GGCGC^C
BsaHI 67 GGCGCC G[GA]^CG[CT]C
BsmFI 980 GGGAC GGGAC(10/14)
BsoBI 872 CTCGGG C^[CT]CG[GA]G
BspLU11I 653 ACATGT A^CATGT
BspMI 389 ACCTGC ACCTGC(4/8)
Bsu36I 664 CCTCAGG CC^T[ACGT]AGG
DraIII 1137 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 253 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 726 TGGCCA [CT]^GGCC[GA]
Eco81I 664 CCTCAGG CC^T[ACGT]AGG
EcoNI 200 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 58 GCGTC GACGC(5/10)
Hin1I 67 GGCGCC G[GA]^CG[CT]C
HinfI 1190 GAATC G^A[ACGT]TC
KasI 67 GGCGCC G^GCGCC
MluNI 726 TGGCCA TGG^CCA
MscI 726 TGGCCA TGG^CCA
NarI 67 GGCGCC GG^CGCC
NcoI 671 CCATGG C^CATGG
NheI 1098 GCTAGC G^CTAGC
PflMI 671 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 186 ATTAAT AT^TAAT
PstI 1177 CTGCAG CTGCA^G
SfcI 1177 CTGCAG C^T[GA][CT]AG
SfiI 744 GGCCGAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 67 GGCGCC GGC^GCC
TfiI 1190 GAATC G^A[AT]TC
Van91I 671 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1186 TCTAGA T^CTAGA
XcmI 224 CCAGGACCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 18 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AlwNI 490 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
758 CAGCAGCTG
Aor51HI 122 AGCGCT AGC^GCT
501 AGCGCT
AspHI 140 GTGCTC G[AT]GC[AT]^C
284 GTGCTC
BanI 67 GGCGCC G^G[CT][GA]CC
660 GGTGCC
BbsI 697 GAAGAC GAAGAC(2/6)
1108 GAAGAC
BcgI 539 CGAATGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1088 CGAAAACATTGC
BcnI 411 CCGGG CC^[GC]GG
1059 CCCGG
BfaI 1099 CTAG C^TAG
1187 CTAG
BglII 20 AGATCT A^GATCT
1181 AGATCT
BmyI 140 GTGCTC G[AGT]GC[ACT]^C
284 GTGCTC
BpuAI 697 GAAGAC GAAGAC(2/6)
1108 GAAGAC
BsiHKAI 140 GTGCTC G[AT]GC[AT]^C
284 GTGCTC
BsmI 540 GAATGC GAATGC(1/-1)
954 GAATGC
Bsp1286I 140 GTGCTC G[AGT]GC[ACT]^C
284 GTGCTC
BsrDI 1094 CATTGC GCAATG(2/0)
544 GCAATG
BsrI 465 ACTGG ACTGG(1/-1)
1140 CCAGT
DraII 635 AGGCCCT [GA]G^G[ACGT]CC[CT]
1164 GGGGCCT
EarI 719 CTCTTC CTCTTC(1/4)
238 GAAGAG
Eco47III 122 AGCGCT AGC^GCT
501 AGCGCT
EcoO109I 635 AGGCCCT [GA]G^G[ACGT]CC[CT]
1164 GGGGCCT
EcoT14I 632 CCAAGG C^C[AT][AT]GG
671 CCATGG
HphI 918 GGTGA GGTGA(8/7)
292 TCACC
Ksp632I 719 CTCTTC CTCTTC(1/4)
238 GAAGAG
KspI 416 CCGCGG CCGC^GG
779 CCGCGG
MaeI 1099 CTAG C^TAG
1187 CTAG
MseI 187 TTAA T^TAA
530 TTAA
NciI 411 CCGGG CC^[GC]GG
1059 CCCGG
NspI 653 ACATGT [GA]CATG^[CT]
1112 ACATGC
SacII 416 CCGCGG CCGC^GG
779 CCGCGG
SmlI 115 CTCAAG C^T[CT][GA]AG
130 CTCAAG
StyI 632 CCAAGG C^C[AT][AT]GG
671 CCATGG
Tru9I 187 TTAA T^TAA
530 TTAA
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AccIII AciI AluI AlwI
AvaII BbvI BglI BpmI BsaJI
BsaWI BseAI BseRI BsgI BsiYI
BslI BsmAI BspEI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MboI MboII MflI MnlI MroI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp509I TspRI TthHB8I XhoII