Restriction map for
pFN21AB6945
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MboI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MvnI NaeI NarI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
Sau3AI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 420 ACACGT A^C[GA][CT]GT
AlwNI 292 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 450 CCTAGG C^CTAGG
BcnI 503 CCCGG CC^[GC]GG
BglI 437 GCCTGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 450 CCTAGG C^CTAGG
BsmI 315 GAATGC GAATGC(1/-1)
BstF5I 561 CATCC GGATG(2/0)
DraII 21 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 389 CTCTTC CTCTTC(1/4)
EcoNI 450 CCTAGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 21 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 464 CCCGC CCCGC(4/6)
FokI 561 CATCC GGATG(9/13)
HapII 504 CCGG C^CGG
HgaI 260 GACGC GACGC(5/10)
HincII 475 GTCGAC GT[CT]^[GA]AC
HindII 475 GTCGAC GT[CT]^[GA]AC
HpaII 504 CCGG C^CGG
Ksp632I 389 CTCTTC CTCTTC(1/4)
MfeI 369 CAATTG C^AATTG
MspI 504 CCGG C^CGG
MunI 369 CAATTG C^AATTG
NciI 503 CCCGG CC^[GC]GG
NcoI 209 CCATGG C^CATGG
PflMI 603 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 268 GACTC GAGTC(4/5)
PpuMI 21 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 298 CTGCAG CTGCA^G
SalI 475 GTCGAC G^TCGAC
SapI 388 GCTCTTC GCTCTTC(1/4)
ScaI 326 AGTACT AGT^ACT
SfcI 298 CTGCAG C^T[GA][CT]AG
TaqI 476 TCGA T^CGA
TfiI 620 GATTC G^A[AT]TC
Tsp509I 370 AATT ^AATT
TspRI 493 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 476 TCGA T^CGA
Van91I 603 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 209 CCATGGGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 424 GTCTAC GT^[AC][GT]AC
475 GTCGAC
AfaI 327 GTAC GT^AC
613 GTAC
AluI 109 AGCT AG^CT
230 AGCT
AvaII 22 GGTCC G^G[AT]CC
556 GGTCC
BfaI 200 CTAG C^TAG
451 CTAG
BseRI 70 GAGGAG GAGGAG(10/8)
94 GAGGAG
BsrDI 498 CATTGC GCAATG(2/0)
551 GCAATG
BsrI 442 ACTGG ACTGG(1/-1)
603 CCAGT
BstAPI 291 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
310 GCAGAGAATGC
BstEII 180 GGTCACC G^GT[ACGT]ACC
459 GGTGACC
BstPI 180 GGTCACC G^GT[ACGT]ACC
459 GGTGACC
Cac8I 48 GCCTGC GC[ACGT]^[ACGT]GC
231 GCTCGC
DdeI 68 CTGAG C^T[ACGT]AG
270 CTCAG
DsaI 209 CCATGG C^C[GA][CT]GG
305 CCACGG
EcoO65I 180 GGTCACC G^GT[ACGT]ACC
459 GGTGACC
EcoT14I 209 CCATGG C^C[AT][AT]GG
450 CCTAGG
HinfI 268 GACTC G^A[ACGT]TC
620 GATTC
MaeI 200 CTAG C^TAG
451 CTAG
MaeIII 181 GTCAC ^GT[ACGT]AC
460 GTGAC
NlaIV 374 GGGGCC GG[ACGT]^[ACGT]CC
556 GGTCCC
RsaI 327 GTAC GT^AC
613 GTAC
SfaNI 365 GCATC GCATC(5/9)
489 GCATC
StyI 209 CCATGG C^C[AT][AT]GG
450 CCTAGG
Tsp45I 181 GTCAC ^GT[GC]AC
460 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BsmFI BspMI BssKI BstNI Cfr13I
EcoRII Fnu4HI HaeIII HphI ItaI
MaeII MboII MnlI MvaI MwoI
NlaIII Sau96I ScrFI TseI