Restriction map for
pFN21AE1388
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AvaII AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BssHII BstAPI BstEII BstPI BstUI
CelII CfoI ClaI CpoI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NcoI NgoMIV NotI
NruI PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AciI 88 CCGC CCGC(-3/-1)
AgeI 1049 ACCGGT A^CCGGT
AhdI 779 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 331 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 779 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1309 GAGCTC G[AT]GC[AT]^C
BalI 918 TGGCCA TGG^CCA
BamHI 20 GGATCC G^GATCC
BanII 1309 GAGCTC G[GA]GC[CT]^C
BcnI 126 CCGGG CC^[GC]GG
BmyI 1309 GAGCTC G[AGT]GC[ACT]^C
BsaJI 126 CCGGGG C^C[ACGT][ACGT]GG
BsaWI 1049 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1309 GAGCTC G[AT]GC[AT]^C
BsmAI 1517 GTCTC GTCTC(1/5)
Bsp1286I 1309 GAGCTC G[AGT]GC[ACT]^C
BsrDI 1314 CATTGC GCAATG(2/0)
BsrFI 1049 ACCGGT [GA]^CCGG[CT]
BssSI 109 CTCGTG CACGAG(-5/-1)
Bst1107I 468 GTATAC GTA^TAC
BstBI 880 TTCGAA TT^CGAA
BstZ17I 468 GTATAC GTA^TAC
Cfr10I 1049 ACCGGT [GA]^CCGG[CT]
Cfr13I 68 GGCCC G^G[ACGT]CC
EaeI 918 TGGCCA [CT]^GGCC[GA]
Eam1105I 779 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1306 GAAGAG CTCTTC(1/4)
EcoT22I 618 ATGCAT ATGCA^T
HgaI 408 GCGTC GACGC(5/10)
HindIII 400 AAGCTT A^AGCTT
Ksp632I 1306 GAAGAG CTCTTC(1/4)
MaeII 215 ACGT A^CGT
MfeI 1172 CAATTG C^AATTG
MluNI 918 TGGCCA TGG^CCA
MscI 918 TGGCCA TGG^CCA
MslI 568 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 76 CAGCTG C[AC]G^C[GT]G
MunI 1172 CAATTG C^AATTG
NciI 126 CCGGG CC^[GC]GG
NdeI 1025 CATATG CA^TATG
NheI 927 GCTAGC G^CTAGC
NlaIV 20 GGATCC GG[ACGT]^[ACGT]CC
NsiI 618 ATGCAT ATGCA^T
NspI 90 GCATGT [GA]CATG^[CT]
NspV 880 TTCGAA TT^CGAA
PflMI 456 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1049 ACCGGT A^CCGGT
PleI 107 GACTC GAGTC(4/5)
PstI 118 CTGCAG CTGCA^G
PvuII 76 CAGCTG CAG^CTG
SacI 1309 GAGCTC GAGCT^C
SapI 1306 GAAGAGC GCTCTTC(1/4)
Sau96I 68 GGCCC G^G[ACGT]CC
SexAI 274 ACCAGGT A^CC[AT]GGT
SfuI 880 TTCGAA TT^CGAA
SpeI 154 ACTAGT A^CTAGT
Tsp45I 151 GTGAC ^GT[GC]AC
Van91I 456 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 331 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 426 GTCTAC GT^[AC][GT]AC
468 GTATAC
AcsI 1114 AAATTT [GA]^AATT[CT]
1330 GAATTT
AocI 56 CCTCAGG CC^T[ACGT]AGG
478 CCTCAGG
ApoI 1114 AAATTT [GA]^AATT[CT]
1330 GAATTT
BclI 95 TGATCA T^GATCA
1335 TGATCA
BpmI 7 CTGGAG CTGGAG(16/14)
755 CTGGAG
BseRI 476 CTCCTC GAGGAG(10/8)
10 GAGGAG
BsmFI 105 GGGAC GGGAC(10/14)
611 GTCCC
Bsp1407I 1404 TGTACA T^GTACA
1507 TGTACA
BsrGI 1404 TGTACA T^GTACA
1507 TGTACA
BstXI 71 CCACGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1461 CCAAGAAAATGG
BstYI 20 GGATCC [GA]^GATC[CT]
594 AGATCC
Bsu36I 56 CCTCAGG CC^T[ACGT]AGG
478 CCTCAGG
DdeI 57 CTCAG C^T[ACGT]AG
479 CTCAG
DraI 1138 TTTAAA TTT^AAA
1423 TTTAAA
Eco81I 56 CCTCAGG CC^T[ACGT]AGG
478 CCTCAGG
FbaI 95 TGATCA T^GATCA
1335 TGATCA
HaeIII 68 GGCC GG^CC
919 GGCC
HapII 126 CCGG C^CGG
1050 CCGG
HpaII 126 CCGG C^CGG
1050 CCGG
MaeIII 151 GTGAC ^GT[ACGT]AC
459 GTTAC
MflI 20 GGATCC [GA]^GATC[CT]
594 AGATCC
MspI 126 CCGG C^CGG
1050 CCGG
SfaNI 949 GATGC GCATC(5/9)
50 GCATC
SfcI 118 CTGCAG C^T[GA][CT]AG
1535 CTGTAG
SspBI 1404 TGTACA T^GTACA
1507 TGTACA
XhoII 20 GGATCC [GA]^GATC[CT]
594 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI AlwI AlwNI BbsI
BbvI BfaI BpuAI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
DpnII EcoRII Fnu4HI FokI HinfI
HphI ItaI MaeI MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I