Restriction map for
pFN21AE3643
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI StyI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 179 GGTACC G^GTACC
AciI 367 CCGC CCGC(-3/-1)
AlwI 201 GATCC GGATC(4/5)
AlwNI 500 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 179 GGTACC G^GTACC
AspHI 104 GAGCTC G[AT]GC[AT]^C
BanI 179 GGTACC G^G[CT][GA]CC
BanII 104 GAGCTC G[GA]GC[CT]^C
BmyI 104 GAGCTC G[AGT]GC[ACT]^C
BpmI 513 CTCCAG CTGGAG(16/14)
BsaI 294 GAGACC GGTCTC(1/5)
BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG
BseRI 194 CTCCTC GAGGAG(10/8)
BsgI 31 CTGCAC GTGCAG(16/14)
BsiHKAI 104 GAGCTC G[AT]GC[AT]^C
BsmAI 294 GAGAC GTCTC(1/5)
BsmI 423 GAATGC GAATGC(1/-1)
Bsp1286I 104 GAGCTC G[AGT]GC[ACT]^C
BspMI 98 ACCTGC ACCTGC(4/8)
BsrBI 367 CCGCTC CCGCTC(-3/-3)
BsrI 548 CCAGT ACTGG(1/-1)
BssKI 58 CCAGG ^CC[ACGT]GG
BstNI 58 CCAGG CC^[AT]GG
BstYI 200 AGATCC [GA]^GATC[CT]
DpnII 201 GATC ^GATC
DraI 394 TTTAAA TTT^AAA
EcoRII 58 CCAGG ^CC[AT]GG
FauI 366 CCCGC CCCGC(4/6)
HaeIII 546 GGCC GG^CC
HincII 321 GTTGAC GT[CT]^[GA]AC
HindII 321 GTTGAC GT[CT]^[GA]AC
HphI 508 GGTGA GGTGA(8/7)
KpnI 179 GGTACC GGTAC^C
MaeII 15 ACGT A^CGT
MaeIII 509 GTGAC ^GT[ACGT]AC
MboI 201 GATC ^GATC
MflI 200 AGATCC [GA]^GATC[CT]
MslI 580 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 334 CAGCTG C[AC]G^C[GT]G
MvaI 58 CCAGG CC^[AT]GG
NdeII 201 GATC ^GATC
PleI 511 GACTC GAGTC(4/5)
PvuII 334 CAGCTG CAG^CTG
SacI 104 GAGCTC GAGCT^C
Sau3AI 201 GATC ^GATC
ScrFI 58 CCAGG CC^[ACGT]GG
SmlI 418 CTCAAG C^T[CT][GA]AG
SphI 447 GCATGC GCATG^C
SspI 111 AATATT AAT^ATT
SwaI 393 ATTTAAAT ATTT^AAAT
TfiI 238 GATTC G^A[AT]TC
Tsp45I 509 GTGAC ^GT[GC]AC
TspRI 433 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 280 CCAAAAGGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 200 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 391 GAATTT [GA]^AATT[CT]
574 AAATTT
AfaI 180 GTAC GT^AC
520 GTAC
AflIII 35 ACATGT A^C[GA][CT]GT
67 ACATGT
ApoI 391 GAATTT [GA]^AATT[CT]
574 AAATTT
BbvI 30 GCTGC GCAGC(8/12)
336 GCTGC
BfaI 157 CTAG C^TAG
556 CTAG
BspLU11I 35 ACATGT A^CATGT
67 ACATGT
EarI 21 GAAGAG CTCTTC(1/4)
478 GAAGAG
Fnu4HI 30 GCTGC GC^[ACGT]GC
336 GCTGC
HinfI 238 GATTC G^A[ACGT]TC
511 GACTC
ItaI 30 GCTGC GC^[ACGT]GC
336 GCTGC
Ksp632I 21 GAAGAG CTCTTC(1/4)
478 GAAGAG
MaeI 157 CTAG C^TAG
556 CTAG
MboII 21 GAAGA GAAGA(8/7)
478 GAAGA
MseI 152 TTAA T^TAA
395 TTAA
NlaIV 121 GGAGCC GG[ACGT]^[ACGT]CC
179 GGTACC
PflMI 428 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
499 CCAGAGGCTGG
RsaI 180 GTAC GT^AC
520 GTAC
SfaNI 483 GATGC GCATC(5/9)
62 GCATC
Tru9I 152 TTAA T^TAA
395 TTAA
TseI 30 GCTGC G^C[AT]GC
336 GCTGC
Van91I 428 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
499 CCAGAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI BsiYI BslI BstF5I Cac8I
DdeI FokI MnlI NlaIII NspI
Tsp509I