Restriction map for
pFN21AE4351
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I FauI FseI
FspI HapII Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AclI 682 AACGTT AA^CGTT
AlwNI 664 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 750 AGCGCT AGC^GCT
AspHI 22 GTGCTC G[AT]GC[AT]^C
BanII 654 GGGCTC G[GA]GC[CT]^C
BbvI 1021 GCTGC GCAGC(8/12)
BclI 660 TGATCA T^GATCA
BfaI 748 CTAG C^TAG
BglII 255 AGATCT A^GATCT
BpmI 224 CTGGAG CTGGAG(16/14)
BseRI 986 CTCCTC GAGGAG(10/8)
BsiHKAI 22 GTGCTC G[AT]GC[AT]^C
BsiWI 859 CGTACG C^GTACG
Bsp1407I 634 TGTACA T^GTACA
BspMI 1008 ACCTGC ACCTGC(4/8)
BsrGI 634 TGTACA T^GTACA
Cac8I 741 GCTTGC GC[ACGT]^[ACGT]GC
Eco47III 750 AGCGCT AGC^GCT
EcoT22I 305 ATGCAT ATGCA^T
FbaI 660 TGATCA T^GATCA
Fnu4HI 1021 GCTGC GC^[ACGT]GC
HaeIII 265 GGCC GG^CC
HgaI 675 GCGTC GACGC(5/10)
ItaI 1021 GCTGC GC^[ACGT]GC
MaeI 748 CTAG C^TAG
MslI 350 CATATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 305 ATGCAT ATGCA^T
PflMI 554 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 682 AACGTT AA^CGTT
ScaI 614 AGTACT AGT^ACT
SfcI 1088 CTATAG C^T[GA][CT]AG
SspBI 634 TGTACA T^GTACA
TseI 1021 GCTGC G^C[AT]GC
Tsp45I 467 GTGAC ^GT[GC]AC
Van91I 554 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 154 CCGC CCGC(-3/-1)
32 GCGG
AlwI 201 GGATC GGATC(4/5)
448 GGATC
AvaII 151 GGACC G^G[AT]CC
908 GGTCC
BbsI 142 GAAGAC GAAGAC(2/6)
970 GAAGAC
BmyI 22 GTGCTC G[AGT]GC[ACT]^C
654 GGGCTC
BpuAI 142 GAAGAC GAAGAC(2/6)
970 GAAGAC
BsiYI 490 CCAAAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
554 CCACTTCATGG
BslI 490 CCAAAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
554 CCACTTCATGG
BsmAI 227 GAGAC GTCTC(1/5)
915 GTCTC
Bsp1286I 22 GTGCTC G[AGT]GC[ACT]^C
654 GGGCTC
BstF5I 899 CATCC GGATG(2/0)
1080 GGATG
BstYI 255 AGATCT [GA]^GATC[CT]
448 GGATCT
Cfr13I 151 GGACC G^G[ACGT]CC
908 GGTCC
EarI 606 CTCTTC CTCTTC(1/4)
76 GAAGAG
EcoRV 174 GATATC GAT^ATC
826 GATATC
FokI 899 CATCC GGATG(9/13)
1080 GGATG
HaeII 3 GGCGCT [GA]GCGC^[CT]
750 AGCGCT
HphI 301 GGTGA GGTGA(8/7)
178 TCACC
Ksp632I 606 CTCTTC CTCTTC(1/4)
76 GAAGAG
MflI 255 AGATCT [GA]^GATC[CT]
448 GGATCT
MseI 416 TTAA T^TAA
473 TTAA
MwoI 878 GCTACATCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1012 GCTAAGGTAGC
PvuII 182 CAGCTG CAG^CTG
630 CAGCTG
Sau96I 151 GGACC G^G[ACGT]CC
908 GGTCC
SfaNI 304 GATGC GCATC(5/9)
307 GCATC
TaqI 164 TCGA T^CGA
1038 TCGA
Tru9I 416 TTAA T^TAA
473 TTAA
TthHB8I 164 TCGA T^CGA
1038 TCGA
XhoII 255 AGATCT [GA]^GATC[CT]
448 GGATCT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsaJI
BsmFI BssKI BstNI CfoI DdeI
DpnII EcoRII HhaI HinP1I HinfI
MaeII MaeIII MboI MboII MnlI
MspA1I MvaI NdeII NlaIII NlaIV
PleI RsaI Sau3AI ScrFI TfiI
Tsp509I TspRI