Restriction map for
pFN21AB7768
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI MaeI MamI
MfeI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 1156 GACGTC GACGT^C
Acc65I 446 GGTACC G^GTACC
AflIII 455 ACGCGT A^C[GA][CT]GT
AlwI 1164 GGATC GGATC(4/5)
Asp718I 446 GGTACC G^GTACC
AspHI 751 GAGCTC G[AT]GC[AT]^C
AvaI 523 CCCGAG C^[CT]CG[GA]G
BanI 446 GGTACC G^G[CT][GA]CC
BbrPI 352 CACGTG CAC^GTG
BbsI 46 GAAGAC GAAGAC(2/6)
BbvCI 57 GCTGAGG CCTCAGC(-5/-2)
BlpI 622 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 622 GCTGAGC GC^T[ACGT]AGC
BpuAI 46 GAAGAC GAAGAC(2/6)
BsaAI 352 CACGTG [CT]AC^GT[GA]
BseRI 315 CTCCTC GAGGAG(10/8)
BsiEI 600 CGACCG CG[GA][CT]^CG
BsiHKAI 751 GAGCTC G[AT]GC[AT]^C
BsmFI 1012 GTCCC GGGAC(10/14)
BsoBI 523 CCCGAG C^[CT]CG[GA]G
BspMI 289 ACCTGC ACCTGC(4/8)
BsrBI 568 GAGCGG CCGCTC(-3/-3)
BssHII 618 GCGCGC G^CGCGC
BssSI 193 CACGAG CACGAG(-5/-1)
BstEII 846 GGTGACC G^GT[ACGT]ACC
BstPI 846 GGTGACC G^GT[ACGT]ACC
BstXI 239 CCAGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1164 GGATCT [GA]^GATC[CT]
CelII 622 GCTGAGC GC^T[ACGT]AGC
DpnII 1165 GATC ^GATC
DraIII 841 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 922 CCGCGG C^C[GA][CT]GG
EaeI 401 TGGCCG [CT]^GGCC[GA]
EcoNI 219 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 846 GGTGACC G^GT[ACGT]ACC
HincII 253 GTCAAC GT[CT]^[GA]AC
HindII 253 GTCAAC GT[CT]^[GA]AC
KpnI 446 GGTACC GGTAC^C
KspI 922 CCGCGG CCGC^GG
MboI 1165 GATC ^GATC
MflI 1164 GGATCT [GA]^GATC[CT]
MluI 455 ACGCGT A^CGCGT
NaeI 740 GCCGGC GCC^GGC
NdeII 1165 GATC ^GATC
NgoMIV 740 GCCGGC G^CCGGC
NlaIII 990 CATG CATG^
NruI 1174 TCGCGA TCG^CGA
PmaCI 352 CACGTG CAC^GTG
PmlI 352 CACGTG CAC^GTG
PpuMI 983 AGGACCC [GA]G^G[AT]CC[CT]
PstI 970 CTGCAG CTGCA^G
SacI 751 GAGCTC GAGCT^C
SacII 922 CCGCGG CCGC^GG
SapI 588 GCTCTTC GCTCTTC(1/4)
Sau3AI 1165 GATC ^GATC
ScaI 197 AGTACT AGT^ACT
StuI 61 AGGCCT AGG^CCT
TfiI 996 GATTC G^A[AT]TC
Tsp509I 171 AATT ^AATT
XcmI 450 CCAGGACGCGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1164 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AocI 1015 CCTGAGG CC^T[ACGT]AGG
1054 CCTGAGG
BsaI 953 GAGACC GGTCTC(1/5)
1122 GGTCTC
BsrFI 740 GCCGGC [GA]^CCGG[CT]
942 GCCGGT
Bsu36I 1015 CCTGAGG CC^T[ACGT]AGG
1054 CCTGAGG
Cfr10I 740 GCCGGC [GA]^CCGG[CT]
942 GCCGGT
DrdI 496 GACGCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1063 GACCTGGGTGTC
EarI 326 CTCTTC CTCTTC(1/4)
589 CTCTTC
Eco81I 1015 CCTGAGG CC^T[ACGT]AGG
1054 CCTGAGG
EcoT14I 68 CCTTGG C^C[AT][AT]GG
1217 CCAAGG
HaeII 813 GGCGCT [GA]GCGC^[CT]
915 GGCGCT
Ksp632I 326 CTCTTC CTCTTC(1/4)
589 CTCTTC
MslI 672 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1111 CACACCAGTG
SexAI 50 ACCAGGT A^CC[AT]GGT
842 ACCTGGT
StyI 68 CCTTGG C^C[AT][AT]GG
1217 CCAAGG
TaqI 782 TCGA T^CGA
1002 TCGA
TthHB8I 782 TCGA T^CGA
1002 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AvaII BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsgI BsiYI BslI BsmAI BsmBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV PleI PvuII RsaI
Sau96I ScrFI SfcI TseI Tsp45I
TspRI