Restriction map for
pFN21AE6344
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraII EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 30 GACGTC GACGT^C
Aor51HI 479 AGCGCT AGC^GCT
AspI 973 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 44 CCCGGG C^[CT]CG[GA]G
BanI 61 GGCGCC G^G[CT][GA]CC
BanII 959 GAGCTC G[GA]GC[CT]^C
BbeI 61 GGCGCC GGCGC^C
BcgI 855 GCAGGACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 241 CTAG C^TAG
BpmI 508 CTGGAG CTGGAG(16/14)
BsaBI 612 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 94 GTGCAG GTGCAG(16/14)
BsmAI 1043 GAGAC GTCTC(1/5)
BsoBI 44 CCCGGG C^[CT]CG[GA]G
BspDI 619 ATCGAT AT^CGAT
BsrDI 368 GCAATG GCAATG(2/0)
BstF5I 534 CATCC GGATG(2/0)
ClaI 619 ATCGAT AT^CGAT
DraIII 604 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 30 GACGTCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 844 CCGTGG C^C[GA][CT]GG
EarI 570 GAAGAG CTCTTC(1/4)
Eco47III 479 AGCGCT AGC^GCT
EcoRI 80 GAATTC G^AATTC
EcoT14I 437 CCTTGG C^C[AT][AT]GG
FokI 534 CATCC GGATG(9/13)
KasI 61 GGCGCC G^GCGCC
Ksp632I 570 GAAGAG CTCTTC(1/4)
MaeI 241 CTAG C^TAG
MaeII 31 ACGT A^CGT
MamI 612 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 61 GGCGCC GG^CGCC
NlaIII 673 CATG CATG^
PflFI 973 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 959 GAGCTC GAGCT^C
SapI 570 GAAGAGC GCTCTTC(1/4)
ScaI 425 AGTACT AGT^ACT
SexAI 605 ACCTGGT A^CC[AT]GGT
SfaNI 433 GATGC GCATC(5/9)
SfoI 61 GGCGCC GGC^GCC
SmaI 44 CCCGGG CCC^GGG
StyI 437 CCTTGG C^C[AT][AT]GG
Tth111I 973 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 36 CGCG CG^CG
70 CGCG
AcsI 80 GAATTC [GA]^AATT[CT]
346 AAATTT
AcyI 30 GACGTC G[GA]^CG[CT]C
61 GGCGCC
ApoI 80 GAATTC [GA]^AATT[CT]
346 AAATTT
AspHI 442 GAGCAC G[AT]GC[AT]^C
959 GAGCTC
AvaII 552 GGACC G^G[AT]CC
639 GGTCC
BsaHI 30 GACGTC G[GA]^CG[CT]C
61 GGCGCC
BsiHKAI 442 GAGCAC G[AT]GC[AT]^C
959 GAGCTC
BsmI 714 GAATGC GAATGC(1/-1)
926 GCATTC
Bsp1407I 404 TGTACA T^GTACA
518 TGTACA
BspMI 593 ACCTGC ACCTGC(4/8)
878 ACCTGC
BsrGI 404 TGTACA T^GTACA
518 TGTACA
BsrI 202 ACTGG ACTGG(1/-1)
888 ACTGG
BstUI 36 CGCG CG^CG
70 CGCG
BstYI 666 AGATCC [GA]^GATC[CT]
1052 GGATCT
DdeI 429 CTCAG C^T[ACGT]AG
740 CTGAG
FauI 58 GCGGG CCCGC(4/6)
265 GCGGG
HaeII 61 GGCGCC [GA]GCGC^[CT]
479 AGCGCT
HgaI 37 GCGTC GACGC(5/10)
575 GCGTC
Hin1I 30 GACGTC G[GA]^CG[CT]C
61 GGCGCC
MflI 666 AGATCC [GA]^GATC[CT]
1052 GGATCT
MvnI 36 CGCG CG^CG
70 CGCG
NlaIV 11 GGCTCC GG[ACGT]^[ACGT]CC
61 GGCGCC
PstI 391 CTGCAG CTGCA^G
421 CTGCAG
SmlI 788 CTCAAG C^T[CT][GA]AG
850 CTCAAG
SspBI 404 TGTACA T^GTACA
518 TGTACA
TaqI 620 TCGA T^CGA
833 TCGA
TthHB8I 620 TCGA T^CGA
833 TCGA
XhoII 666 AGATCC [GA]^GATC[CT]
1052 GGATCT
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII RsaI Sau3AI Sau96I
ScrFI SfcI TfiI TseI Tsp45I
Tsp509I TspRI