Restriction map for
pFN21AE5125
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HgaI
HincII HindII HindIII HinfI HpaI
KpnI KspI MaeI MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MspA1I MunI NciI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SfaNI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 144 CGCG CG^CG
AcyI 12 GGCGCC G[GA]^CG[CT]C
AfaI 193 GTAC GT^AC
Alw44I 169 GTGCAC G^TGCAC
ApaLI 169 GTGCAC G^TGCAC
AspHI 169 GTGCAC G[AT]GC[AT]^C
AvaII 71 GGTCC G^G[AT]CC
BanI 12 GGCGCC G^G[CT][GA]CC
BbeI 12 GGCGCC GGCGC^C
BbvI 27 GCAGC GCAGC(8/12)
BmyI 169 GTGCAC G[AGT]GC[ACT]^C
BsaHI 12 GGCGCC G[GA]^CG[CT]C
BsaI 43 GAGACC GGTCTC(1/5)
BsiHKAI 169 GTGCAC G[AT]GC[AT]^C
BsmAI 43 GAGAC GTCTC(1/5)
Bsp1286I 169 GTGCAC G[AGT]GC[ACT]^C
BspHI 222 TCATGA T^CATGA
BspMI 197 ACCTGC ACCTGC(4/8)
BsrFI 30 GCCGGC [GA]^CCGG[CT]
BstUI 144 CGCG CG^CG
Cac8I 30 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 13 GCGC GCG^C
Cfr10I 30 GCCGGC [GA]^CCGG[CT]
Cfr13I 71 GGTCC G^G[ACGT]CC
DraII 70 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 32 CGGCCA [CT]^GGCC[GA]
EarI 82 GAAGAG CTCTTC(1/4)
EcoO109I 70 AGGTCCC [GA]G^G[ACGT]CC[CT]
Fnu4HI 27 GCAGC GC^[ACGT]GC
HaeII 12 GGCGCC [GA]GCGC^[CT]
HapII 31 CCGG C^CGG
HhaI 13 GCGC GCG^C
Hin1I 12 GGCGCC G[GA]^CG[CT]C
HinP1I 13 GCGC G^CGC
HpaII 31 CCGG C^CGG
HphI 66 GGTGA GGTGA(8/7)
ItaI 27 GCAGC GC^[ACGT]GC
KasI 12 GGCGCC G^GCGCC
Ksp632I 82 GAAGAG CTCTTC(1/4)
MaeII 173 ACGT A^CGT
MboII 82 GAAGA GAAGA(8/7)
MseI 113 TTAA T^TAA
MslI 162 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 31 CCGG C^CGG
MvnI 144 CGCG CG^CG
NaeI 30 GCCGGC GCC^GGC
NarI 12 GGCGCC GG^CGCC
NgoMIV 30 GCCGGC G^CCGGC
PpuMI 70 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 222 TCATGA T^CATGA
RsaI 193 GTAC GT^AC
Sau96I 71 GGTCC G^G[ACGT]CC
SexAI 62 ACCAGGT A^CC[AT]GGT
SfoI 12 GGCGCC GGC^GCC
Tru9I 113 TTAA T^TAA
TseI 27 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 77 AGCT AG^CT
281 AGCT
BsmFI 148 GGGAC GGGAC(10/14)
72 GTCCC
BssKI 63 CCAGG ^CC[ACGT]GG
135 CCAGG
BstNI 63 CCAGG CC^[AT]GG
135 CCAGG
EcoRII 63 CCAGG ^CC[AT]GG
135 CCAGG
FauI 8 GCGGG CCCGC(4/6)
145 GCGGG
HaeIII 33 GGCC GG^CC
117 GGCC
MvaI 63 CCAGG CC^[AT]GG
135 CCAGG
MwoI 13 GCGCCCTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
273 GCATGATGAGC
NlaIII 223 CATG CATG^
274 CATG
NlaIV 12 GGCGCC GG[ACGT]^[ACGT]CC
71 GGTCCC
ScrFI 63 CCAGG CC^[ACGT]GG
135 CCAGG
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
AciI MnlI