Restriction map for
pFN21AE5517
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BanI BbeI BbrPI
BcgI BciVI BfrI BglII BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HaeII HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MslI
MunI NarI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1201 GTAGAC GT^[AC][GT]AC
AccII 4 CGCG CG^CG
AcsI 716 GAATTC [GA]^AATT[CT]
AcyI 1077 GGCGTC G[GA]^CG[CT]C
ApoI 716 GAATTC [GA]^AATT[CT]
AspHI 956 GAGCTC G[AT]GC[AT]^C
AvaI 167 CTCGAG C^[CT]CG[GA]G
AvrII 310 CCTAGG C^CTAGG
BamHI 1143 GGATCC G^GATCC
BbsI 667 GAAGAC GAAGAC(2/6)
BbvCI 1251 GCTGAGG CCTCAGC(-5/-2)
BclI 780 TGATCA T^GATCA
BcnI 19 CCGGG CC^[GC]GG
BlnI 310 CCTAGG C^CTAGG
BpuAI 667 GAAGAC GAAGAC(2/6)
BsaHI 1077 GGCGTC G[GA]^CG[CT]C
BsgI 301 GTGCAG GTGCAG(16/14)
BsiHKAI 956 GAGCTC G[AT]GC[AT]^C
BsoBI 167 CTCGAG C^[CT]CG[GA]G
BstAPI 1111 GCAAAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 149 CATCC GGATG(2/0)
BstUI 4 CGCG CG^CG
DraI 1084 TTTAAA TTT^AAA
DraIII 698 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 920 CCATGG C^C[GA][CT]GG
EarI 831 GAAGAG CTCTTC(1/4)
EcoNI 173 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 716 GAATTC G^AATTC
FauI 962 CCCGC CCCGC(4/6)
FbaI 780 TGATCA T^GATCA
FokI 149 CATCC GGATG(9/13)
HgaI 1078 GCGTC GACGC(5/10)
Hin1I 1077 GGCGTC G[GA]^CG[CT]C
HindIII 1168 AAGCTT A^AGCTT
Ksp632I 831 GAAGAG CTCTTC(1/4)
MaeII 777 ACGT A^CGT
MvnI 4 CGCG CG^CG
NciI 19 CCGGG CC^[GC]GG
NcoI 920 CCATGG C^CATGG
NdeI 1282 CATATG CA^TATG
NspI 1212 GCATGT [GA]CATG^[CT]
PaeR7I 167 CTCGAG C^TCGAG
PleI 490 GACTC GAGTC(4/5)
PpuMI 1300 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 588 CAGCTG CAG^CTG
SacI 956 GAGCTC GAGCT^C
SapI 831 GAAGAGC GCTCTTC(1/4)
SmlI 167 CTCGAG C^T[CT][GA]AG
SspI 1088 AATATT AAT^ATT
SwaI 1083 ATTTAAAT ATTT^AAAT
TaqI 168 TCGA T^CGA
TthHB8I 168 TCGA T^CGA
XhoI 167 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BalI 386 TGGCCA TGG^CCA
1001 TGGCCA
BanII 351 GAGCCC G[GA]GC[CT]^C
956 GAGCTC
BglI 306 GCCTCCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1120 GCCATTGAGGC
BmyI 351 GAGCCC G[AGT]GC[ACT]^C
956 GAGCTC
BpmI 67 CTGGAG CTGGAG(16/14)
332 CTGGAG
BseRI 1025 CTCCTC GAGGAG(10/8)
13 GAGGAG
BsiYI 173 CCTGTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
963 CCGCAGCGTGG
BslI 173 CCTGTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
963 CCGCAGCGTGG
BsmFI 362 GTCCC GGGAC(10/14)
564 GTCCC
Bsp1286I 351 GAGCCC G[AGT]GC[ACT]^C
956 GAGCTC
BsrFI 60 GCCGGC [GA]^CCGG[CT]
1263 GCCGGC
BstYI 194 GGATCT [GA]^GATC[CT]
1143 GGATCC
CfoI 3 GCGC GCG^C
1210 GCGC
Cfr10I 60 GCCGGC [GA]^CCGG[CT]
1263 GCCGGC
DraII 21 GGGGCCT [GA]G^G[ACGT]CC[CT]
1300 GGGTCCT
EcoO109I 21 GGGGCCT [GA]G^G[ACGT]CC[CT]
1300 GGGTCCT
HhaI 3 GCGC GCG^C
1210 GCGC
HinP1I 3 GCGC G^CGC
1210 GCGC
MflI 194 GGATCT [GA]^GATC[CT]
1143 GGATCC
MluNI 386 TGGCCA TGG^CCA
1001 TGGCCA
MscI 386 TGGCCA TGG^CCA
1001 TGGCCA
MspA1I 588 CAGCTG C[AC]G^C[GT]G
1139 CAGCGG
NaeI 60 GCCGGC GCC^GGC
1263 GCCGGC
NgoMIV 60 GCCGGC G^CCGGC
1263 GCCGGC
SfcI 32 CTACAG C^T[GA][CT]AG
1199 CTGTAG
XhoII 194 GGATCT [GA]^GATC[CT]
1143 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BsaJI BsmAI BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI