Restriction map for
pFN21AE1329
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbrPI BcgI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI DraIII Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SfiI SfuI SgrAI SnaBI
SspBI SspI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 335 GGTACC G^GTACC
AccI 625 GTCTAC GT^[AC][GT]AC
AccII 271 CGCG CG^CG
AcyI 616 GGCGCC G[GA]^CG[CT]C
AflIII 347 ACATGT A^C[GA][CT]GT
Alw44I 1357 GTGCAC G^TGCAC
AocI 611 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1487 AGCGCT AGC^GCT
ApaLI 1357 GTGCAC G^TGCAC
Asp718I 335 GGTACC G^GTACC
BamHI 164 GGATCC G^GATCC
BbeI 616 GGCGCC GGCGC^C
BciVI 717 GGATAC GTATCC(6/5)
BglI 1379 GCCGCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1229 AGATCT A^GATCT
BsaAI 1531 TACGTG [CT]AC^GT[GA]
BsaHI 616 GGCGCC G[GA]^CG[CT]C
BsiEI 1377 CGGCCG CG[GA][CT]^CG
BsmBI 367 GAGACG CGTCTC(1/5)
BspHI 1049 TCATGA T^CATGA
BspLU11I 347 ACATGT A^CATGT
BsrBI 487 CCGCTC CCGCTC(-3/-3)
BsrFI 1369 GCCGGC [GA]^CCGG[CT]
BssSI 651 CACGAG CACGAG(-5/-1)
BstUI 271 CGCG CG^CG
Bsu36I 611 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1369 GCCGGC [GA]^CCGG[CT]
DrdI 1261 GACCATGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1377 CGGCCG C^GGCCG
EarI 1764 GAAGAG CTCTTC(1/4)
EclXI 1377 CGGCCG C^GGCCG
Eco47III 1487 AGCGCT AGC^GCT
Eco52I 1377 CGGCCG C^GGCCG
Eco81I 611 CCTCAGG CC^T[ACGT]AGG
Hin1I 616 GGCGCC G[GA]^CG[CT]C
KasI 616 GGCGCC G^GCGCC
KpnI 335 GGTACC GGTAC^C
Ksp632I 1764 GAAGAG CTCTTC(1/4)
MvnI 271 CGCG CG^CG
NaeI 1369 GCCGGC GCC^GGC
NarI 616 GGCGCC GG^CGCC
NgoMIV 1369 GCCGGC G^CCGGC
PflMI 786 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 934 CAGCTG CAG^CTG
RcaI 1049 TCATGA T^CATGA
SapI 1764 GAAGAGC GCTCTTC(1/4)
ScaI 236 AGTACT AGT^ACT
SexAI 572 ACCTGGT A^CC[AT]GGT
SfoI 616 GGCGCC GGC^GCC
SmaI 895 CCCGGG CCC^GGG
SpeI 322 ACTAGT A^CTAGT
SphI 1112 GCATGC GCATG^C
TfiI 1221 GATTC G^A[AT]TC
Van91I 786 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 895 CCCGGG C^CCGGG
XmaI 895 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwNI 787 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1243 CAGTACCTG
ApaI 1129 GGGCCC GGGCC^C
1345 GGGCCC
AspHI 451 GTGCTC G[AT]GC[AT]^C
1357 GTGCAC
BalI 342 TGGCCA TGG^CCA
1535 TGGCCA
BbsI 591 GAAGAC GAAGAC(2/6)
1461 GAAGAC
BbvCI 420 CCTCAGC CCTCAGC(-5/-2)
1293 CCTCAGC
BcnI 895 CCCGG CC^[GC]GG
896 CCGGG
BfaI 323 CTAG C^TAG
940 CTAG
BpuAI 591 GAAGAC GAAGAC(2/6)
1461 GAAGAC
BsgI 1705 CTGCAC GTGCAG(16/14)
1501 GTGCAG
BsiHKAI 451 GTGCTC G[AT]GC[AT]^C
1357 GTGCAC
BsmI 660 GAATGC GAATGC(1/-1)
1644 GAATGC
BspMI 629 ACCTGC ACCTGC(4/8)
1703 ACCTGC
BstEII 150 GGTGACC G^GT[ACGT]ACC
495 GGTCACC
BstPI 150 GGTGACC G^GT[ACGT]ACC
495 GGTCACC
BstYI 164 GGATCC [GA]^GATC[CT]
1229 AGATCT
EcoNI 54 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1336 CCTCACGCAGG
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
495 GGTCACC
MaeI 323 CTAG C^TAG
940 CTAG
MaeII 400 ACGT A^CGT
1532 ACGT
MflI 164 GGATCC [GA]^GATC[CT]
1229 AGATCT
MluNI 342 TGGCCA TGG^CCA
1535 TGGCCA
MscI 342 TGGCCA TGG^CCA
1535 TGGCCA
NciI 895 CCCGG CC^[GC]GG
896 CCGGG
NcoI 339 CCATGG C^CATGG
1263 CCATGG
PpuMI 1268 GGGTCCC [GA]G^G[AT]CC[CT]
1577 AGGTCCT
SbfI 261 CCTGCAGG CCTGCA^GG
870 CCTGCAGG
SfaNI 43 GATGC GCATC(5/9)
1373 GCATC
Sse8387I 261 CCTGCAGG CCTGCA^GG
870 CCTGCAGG
StuI 608 AGGCCT AGG^CCT
1658 AGGCCT
TaqI 557 TCGA T^CGA
1403 TCGA
TthHB8I 557 TCGA T^CGA
1403 TCGA
XhoII 164 GGATCC [GA]^GATC[CT]
1229 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanI BanII BbvI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
StyI TseI Tsp45I TspRI XcmI