Restriction map for
pFN21AE6305
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BalI BanII
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglII BpuAI BsaAI BsaBI
BsaI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspLU11I BsrDI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI BstXI Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HgaI HindIII HpaI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 1092 GTATAC GT^[AC][GT]AC
AcyI 104 GGCGCC G[GA]^CG[CT]C
AflIII 546 ACGTGT A^C[GA][CT]GT
AocI 933 CCTGAGG CC^T[ACGT]AGG
Asp700I 989 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 276 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 647 CTCGGG C^[CT]CG[GA]G
AvrII 67 CCTAGG C^CTAGG
BamHI 899 GGATCC G^GATCC
BanI 104 GGCGCC G^G[CT][GA]CC
BbeI 104 GGCGCC GGCGC^C
BclI 972 TGATCA T^GATCA
BcnI 903 CCCGG CC^[GC]GG
BglI 470 GCCTTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 67 CCTAGG C^CTAGG
BpmI 242 CTGGAG CTGGAG(16/14)
BsaHI 104 GGCGCC G[GA]^CG[CT]C
BsaWI 977 ACCGGA [AT]^CCGG[AT]
BseRI 1142 CTCCTC GAGGAG(10/8)
BsgI 964 GTGCAG GTGCAG(16/14)
BsmAI 780 GAGAC GTCTC(1/5)
BsmBI 780 GAGACG CGTCTC(1/5)
BsoBI 647 CTCGGG C^[CT]CG[GA]G
Bsp1407I 485 TGTACA T^GTACA
BspHI 402 TCATGA T^CATGA
BspMI 802 GCAGGT ACCTGC(4/8)
BsrGI 485 TGTACA T^GTACA
Bst1107I 1092 GTATAC GTA^TAC
BstYI 899 GGATCC [GA]^GATC[CT]
BstZ17I 1092 GTATAC GTA^TAC
Bsu36I 933 CCTGAGG CC^T[ACGT]AGG
DraI 388 TTTAAA TTT^AAA
DraIII 86 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 717 GACCTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1148 TGGCCG [CT]^GGCC[GA]
EarI 1217 GAAGAG CTCTTC(1/4)
Eco81I 933 CCTGAGG CC^T[ACGT]AGG
FauI 198 GCGGG CCCGC(4/6)
FbaI 972 TGATCA T^GATCA
Hin1I 104 GGCGCC G[GA]^CG[CT]C
HincII 273 GTTGAC GT[CT]^[GA]AC
HindII 273 GTTGAC GT[CT]^[GA]AC
KasI 104 GGCGCC G^GCGCC
Ksp632I 1217 GAAGAG CTCTTC(1/4)
MaeII 546 ACGT A^CGT
MflI 899 GGATCC [GA]^GATC[CT]
NarI 104 GGCGCC GG^CGCC
NciI 903 CCCGG CC^[GC]GG
NcoI 959 CCATGG C^CATGG
PflFI 276 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 707 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 908 AGGACCT [GA]G^G[AT]CC[CT]
PstI 225 CTGCAG CTGCA^G
RcaI 402 TCATGA T^CATGA
ScaI 446 AGTACT AGT^ACT
SfoI 104 GGCGCC GGC^GCC
SspBI 485 TGTACA T^GTACA
StuI 477 AGGCCT AGG^CCT
TaqI 292 TCGA T^CGA
Tth111I 276 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 292 TCGA T^CGA
Van91I 707 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 899 GGATCC [GA]^GATC[CT]
XmnI 989 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 147 AGCT AG^CT
709 AGCT
BlpI 193 GCTGAGC GC^T[ACGT]AGC
525 GCTCAGC
Bpu1102I 193 GCTGAGC GC^T[ACGT]AGC
525 GCTCAGC
BsrBI 55 CCGCTC CCGCTC(-3/-3)
196 GAGCGG
BsrI 1186 ACTGG ACTGG(1/-1)
1161 CCAGT
CelII 193 GCTGAGC GC^T[ACGT]AGC
525 GCTCAGC
CfoI 76 GCGC GCG^C
105 GCGC
DraII 28 AGGGCCT [GA]G^G[ACGT]CC[CT]
908 AGGACCT
DsaI 531 CCGTGG C^C[GA][CT]GG
959 CCATGG
EcoO109I 28 AGGGCCT [GA]G^G[ACGT]CC[CT]
908 AGGACCT
HaeII 75 AGCGCC [GA]GCGC^[CT]
104 GGCGCC
HapII 904 CCGG C^CGG
978 CCGG
HhaI 76 GCGC GCG^C
105 GCGC
HinP1I 76 GCGC G^CGC
105 GCGC
HpaII 904 CCGG C^CGG
978 CCGG
MboII 619 GAAGA GAAGA(8/7)
1217 GAAGA
MseI 310 TTAA T^TAA
389 TTAA
MslI 957 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1008 CATTACAGTG
MspI 904 CCGG C^CGG
978 CCGG
SfcI 225 CTGCAG C^T[GA][CT]AG
772 CTACAG
Tru9I 310 TTAA T^TAA
389 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AlwI AlwNI
ApoI AspHI AvaII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstAPI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI TseI Tsp45I Tsp509I TspRI