Restriction map for
pFN21AE1407
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AviII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 60 GGTACC G^GTACC
AlwNI 790 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 964 GGGCCC GGGCC^C
Asp718I 60 GGTACC G^GTACC
AvaI 943 CTCGGG C^[CT]CG[GA]G
AvrII 772 CCTAGG C^CTAGG
BanI 60 GGTACC G^G[CT][GA]CC
BanII 964 GGGCCC G[GA]GC[CT]^C
BfaI 773 CTAG C^TAG
BlnI 772 CCTAGG C^CTAGG
BmyI 964 GGGCCC G[AGT]GC[ACT]^C
BpmI 541 CTGGAG CTGGAG(16/14)
BseRI 712 CTCCTC GAGGAG(10/8)
BsoBI 943 CTCGGG C^[CT]CG[GA]G
Bsp1286I 964 GGGCCC G[AGT]GC[ACT]^C
BspMI 126 ACCTGC ACCTGC(4/8)
BsrBI 909 GAGCGG CCGCTC(-3/-3)
BsrDI 891 CATTGC GCAATG(2/0)
BstEII 247 GGTGACC G^GT[ACGT]ACC
BstPI 247 GGTGACC G^GT[ACGT]ACC
DpnII 589 GATC ^GATC
DsaI 547 CCATGG C^C[GA][CT]GG
EaeI 668 TGGCCG [CT]^GGCC[GA]
EarI 782 CTCTTC CTCTTC(1/4)
EcoO65I 247 GGTGACC G^GT[ACGT]ACC
EcoT22I 195 ATGCAT ATGCA^T
FauI 951 CCCGC CCCGC(4/6)
KpnI 60 GGTACC GGTAC^C
Ksp632I 782 CTCTTC CTCTTC(1/4)
MaeI 773 CTAG C^TAG
MboI 589 GATC ^GATC
MseI 95 TTAA T^TAA
MslI 675 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 933 CAGCTG C[AC]G^C[GT]G
NcoI 547 CCATGG C^CATGG
NdeI 382 CATATG CA^TATG
NdeII 589 GATC ^GATC
NsiI 195 ATGCAT ATGCA^T
PstI 936 CTGCAG CTGCA^G
PvuII 933 CAGCTG CAG^CTG
Sau3AI 589 GATC ^GATC
ScaI 363 AGTACT AGT^ACT
TaqI 573 TCGA T^CGA
Tru9I 95 TTAA T^TAA
TspRI 115 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 573 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AciI 952 CCGC CCGC(-3/-1)
911 GCGG
Asp700I 83 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
493 GAAAAGTTTC
BsmFI 946 GGGAC GGGAC(10/14)
652 GTCCC
BsmI 350 GAATGC GAATGC(1/-1)
288 GCATTC
DrdI 520 GACTTTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
643 GACGAAACGGTC
EcoRI 87 GAATTC G^AATTC
777 GAATTC
MwoI 11 GCCTTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
271 GCTGAATTTGC
PpuMI 527 AGGTCCT [GA]G^G[AT]CC[CT]
946 GGGACCC
SfcI 112 CTACAG C^T[GA][CT]AG
936 CTGCAG
SmlI 228 CTCAAG C^T[CT][GA]AG
258 CTTGAG
TfiI 373 GATTC G^A[AT]TC
921 GAATC
XmnI 83 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
493 GAAAAGTTTC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbsI BbvI BpuAI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboII
MnlI MvaI NlaIII NlaIV PleI
RsaI Sau96I ScrFI StyI TseI
Tsp45I Tsp509I