Restriction map for
pFN21AE1449
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BcnI BfaI BfrI BglI
BlnI BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MfeI MluI MroI MslI
MspA1I MunI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 468 GTCTAC GT^[AC][GT]AC
AccII 1145 CGCG CG^CG
AcsI 68 GAATTC [GA]^AATT[CT]
Alw44I 761 GTGCAC G^TGCAC
AlwNI 899 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 761 GTGCAC G^TGCAC
ApoI 68 GAATTC [GA]^AATT[CT]
BanI 894 GGCACC G^G[CT][GA]CC
BanII 692 GAGCTC G[GA]GC[CT]^C
BcgI 621 CGACAGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 245 GGATAC GTATCC(6/5)
BclI 567 TGATCA T^GATCA
BglII 189 AGATCT A^GATCT
BlpI 937 GCTCAGC GC^T[ACGT]AGC
BpmI 377 CTGGAG CTGGAG(16/14)
Bpu1102I 937 GCTCAGC GC^T[ACGT]AGC
BsaBI 184 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 706 GCAGGT ACCTGC(4/8)
BsrDI 627 CATTGC GCAATG(2/0)
BsrFI 751 GCCGGC [GA]^CCGG[CT]
BstBI 335 TTCGAA TT^CGAA
BstF5I 423 GGATG GGATG(2/0)
BstUI 1145 CGCG CG^CG
BstXI 864 CCAGGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 937 GCTCAGC GC^T[ACGT]AGC
CfoI 1144 GCGC GCG^C
Cfr10I 751 GCCGGC [GA]^CCGG[CT]
DraI 510 TTTAAA TTT^AAA
DraIII 584 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 68 GAATTC G^AATTC
FbaI 567 TGATCA T^GATCA
FokI 423 GGATG GGATG(9/13)
HapII 752 CCGG C^CGG
HhaI 1144 GCGC GCG^C
HinP1I 1144 GCGC G^CGC
HpaII 752 CCGG C^CGG
MamI 184 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 752 CCGG C^CGG
MvnI 1145 CGCG CG^CG
NaeI 751 GCCGGC GCC^GGC
NgoMIV 751 GCCGGC G^CCGGC
NlaIII 1110 CATG CATG^
NspV 335 TTCGAA TT^CGAA
PleI 699 GACTC GAGTC(4/5)
PpuMI 387 AGGACCT [GA]G^G[AT]CC[CT]
PstI 596 CTGCAG CTGCA^G
SacI 692 GAGCTC GAGCT^C
SfaNI 117 GATGC GCATC(5/9)
SfuI 335 TTCGAA TT^CGAA
SmlI 701 CTCAAG C^T[CT][GA]AG
SspI 400 AATATT AAT^ATT
XcmI 1179 CCAACCTGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 772 CCGC CCGC(-3/-1)
943 CCGC
AlwI 505 GATCC GGATC(4/5)
15 GGATC
BalI 273 TGGCCA TGG^CCA
873 TGGCCA
BsgI 974 CTGCAC GTGCAG(16/14)
237 GTGCAG
BsrI 838 ACTGG ACTGG(1/-1)
587 CCAGT
BstYI 15 GGATCT [GA]^GATC[CT]
189 AGATCT
DraII 387 AGGACCT [GA]G^G[ACGT]CC[CT]
1063 GGGGCCT
EaeI 273 TGGCCA [CT]^GGCC[GA]
873 TGGCCA
EcoO109I 387 AGGACCT [GA]G^G[ACGT]CC[CT]
1063 GGGGCCT
HphI 303 GGTGA GGTGA(8/7)
651 TCACC
MaeIII 160 GTAAC ^GT[ACGT]AC
1148 GTAAC
MflI 15 GGATCT [GA]^GATC[CT]
189 AGATCT
MluNI 273 TGGCCA TGG^CCA
873 TGGCCA
MscI 273 TGGCCA TGG^CCA
873 TGGCCA
SexAI 1080 ACCAGGT A^CC[AT]GGT
1188 ACCTGGT
SfcI 596 CTGCAG C^T[GA][CT]AG
825 CTATAG
XhoII 15 GGATCT [GA]^GATC[CT]
189 AGATCT
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI AspHI AvaII BbvCI
BbvI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI ItaI MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIV RsaI Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I