Restriction map for
pFN21AE1389
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsiEI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MboI MfeI MflI MluI
MspA1I MunI MvnI NaeI NarI
NcoI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
StyI SwaI Tth111I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 65 TCCGGA T^CCGGA
ApaI 148 GGGCCC GGGCC^C
AspHI 402 GAGCTC G[AT]GC[AT]^C
AvaI 956 CTCGAG C^[CT]CG[GA]G
AvaII 63 GGTCC G^G[AT]CC
BalI 1073 TGGCCA TGG^CCA
BbsI 580 GAAGAC GAAGAC(2/6)
BcgI 848 GCACAGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 396 CCGGG CC^[GC]GG
BfaI 56 CTAG C^TAG
BpuAI 580 GAAGAC GAAGAC(2/6)
BsaAI 787 TACGTG [CT]AC^GT[GA]
BsaI 959 GAGACC GGTCTC(1/5)
BsaWI 65 TCCGGA [AT]^CCGG[AT]
BseAI 65 TCCGGA T^CCGGA
BsiHKAI 402 GAGCTC G[AT]GC[AT]^C
BsoBI 956 CTCGAG C^[CT]CG[GA]G
Bsp1407I 927 TGTACA T^GTACA
BspEI 65 TCCGGA T^CCGGA
BsrGI 927 TGTACA T^GTACA
BstAPI 1126 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 678 GCATGC GC[ACGT]^[ACGT]GC
DraI 166 TTTAAA TTT^AAA
EaeI 1073 TGGCCA [CT]^GGCC[GA]
EcoNI 561 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 271 GAATTC G^AATTC
EcoRV 208 GATATC GAT^ATC
FauI 407 CCCGC CCCGC(4/6)
MaeI 56 CTAG C^TAG
MluNI 1073 TGGCCA TGG^CCA
MroI 65 TCCGGA T^CCGGA
MscI 1073 TGGCCA TGG^CCA
NciI 396 CCGGG CC^[GC]GG
NdeI 182 CATATG CA^TATG
NspI 678 GCATGC [GA]CATG^[CT]
PaeR7I 956 CTCGAG C^TCGAG
PleI 141 GACTC GAGTC(4/5)
SacI 402 GAGCTC GAGCT^C
SapI 700 GCTCTTC GCTCTTC(1/4)
SphI 678 GCATGC GCATG^C
SspBI 927 TGTACA T^GTACA
XhoI 956 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 390 CCGC CCGC(-3/-1)
408 CCGC
BanII 148 GGGCCC G[GA]GC[CT]^C
402 GAGCTC
BbvCI 287 CCTCAGC CCTCAGC(-5/-2)
695 CCTCAGC
BmyI 148 GGGCCC G[AGT]GC[ACT]^C
402 GAGCTC
Bsp1286I 148 GGGCCC G[AGT]GC[ACT]^C
402 GAGCTC
BspMI 764 ACCTGC ACCTGC(4/8)
938 ACCTGC
EarI 701 CTCTTC CTCTTC(1/4)
812 GAAGAG
HapII 66 CCGG C^CGG
396 CCGG
HpaII 66 CCGG C^CGG
396 CCGG
Ksp632I 701 CTCTTC CTCTTC(1/4)
812 GAAGAG
MaeII 746 ACGT A^CGT
788 ACGT
MslI 197 CATTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
636 CACCCAGGTG
MspI 66 CCGG C^CGG
396 CCGG
MwoI 1075 GCCAAAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1126 GCAGCATTTGC
PflMI 181 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
445 CCACAGTATGG
TaqI 669 TCGA T^CGA
957 TCGA
TspRI 627 CACTG CA[GC]TG[ACGT][ACGT]^
1005 CAGTG
TthHB8I 669 TCGA T^CGA
957 TCGA
Van91I 181 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
445 CCACAGTATGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboII MnlI MseI
MvaI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I