##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraI        DraII       DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaI        HpaII       
HphI        KasI        KpnI        Ksp632I     KspI        
MaeIII      MamI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AlwI          213  GGATC                GGATC(4/5)
AlwNI         285  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
BbvI          137  GCAGC                GCAGC(8/12)
BglII         156  AGATCT               A^GATCT
BsaAI         440  CACGTA               [CT]AC^GT[GA]
BsaJI         236  CCCTGG               C^C[ACGT][ACGT]GG
BsgI          135  GTGCAG               GTGCAG(16/14)
BsmFI          58  GTCCC                GGGAC(10/14)
BssKI         237  CCTGG                ^CC[ACGT]GG
BstNI         237  CCTGG                CC^[AT]GG
BstYI         156  AGATCT               [GA]^GATC[CT]
Cac8I         405  GCAGGC               GC[ACGT]^[ACGT]GC
DraIII        440  CACGTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRII        237  CCTGG                ^CC[AT]GG
EcoRV         301  GATATC               GAT^ATC
Fnu4HI        137  GCAGC                GC^[ACGT]GC
HindIII        34  AAGCTT               A^AGCTT
ItaI          137  GCAGC                GC^[ACGT]GC
MaeII         441  ACGT                 A^CGT
MfeI          398  CAATTG               C^AATTG
MflI          156  AGATCT               [GA]^GATC[CT]
MslI          250  CATCGTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          398  CAATTG               C^AATTG
MvaI          237  CCTGG                CC^[AT]GG
MwoI           36  GCTTCGACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PstI          418  CTGCAG               CTGCA^G
ScrFI         237  CCTGG                CC^[ACGT]GG
SfcI          418  CTGCAG               C^T[GA][CT]AG
SmlI          145  CTTGAG               C^T[CT][GA]AG
TaqI           39  TCGA                 T^CGA
TseI          137  GCAGC                G^C[AT]GC
TspRI         285  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        39  TCGA                 T^CGA
XhoII         156  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

Asp700I        97  GAACTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              241  GAAATAATTC          
BfaI          185  CTAG                 C^TAG
              298  CTAG                
BstF5I        374  CATCC                GGATG(2/0)
              339  GGATG               
FokI          374  CATCC                GGATG(9/13)
              339  GGATG               
MaeI          185  CTAG                 C^TAG
              298  CTAG                
MseI          311  TTAA                 T^TAA
              392  TTAA                
Tru9I         311  TTAA                 T^TAA
              392  TTAA                
XmnI           97  GAACTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              241  GAAATAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        DpnII       MboI        MboII       MnlI        
NdeII       Sau3AI      Tsp509I