Restriction map for
pFN21AE1385
##################################
# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AluI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
ItaI KasI KpnI KspI MfeI
MflI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 331 GTCTAC GT^[AC][GT]AC
AciI 242 CCGC CCGC(-3/-1)
AspI 103 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 36 GGCACC G^G[CT][GA]CC
BclI 71 TGATCA T^GATCA
BcnI 218 CCGGG CC^[GC]GG
BfaI 188 CTAG C^TAG
BsaBI 72 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 88 GAGACC GGTCTC(1/5)
BsmAI 88 GAGAC GTCTC(1/5)
BsmI 166 GCATTC GAATGC(1/-1)
BspHI 284 TCATGA T^CATGA
BstNI 225 CCAGG CC^[AT]GG
DpnII 72 GATC ^GATC
DrdI 103 GACCATGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 133 CTCTTC CTCTTC(1/4)
EcoRII 225 CCAGG ^CC[AT]GG
EcoT14I 58 CCAAGG C^C[AT][AT]GG
FbaI 71 TGATCA T^GATCA
HapII 218 CCGG C^CGG
HpaII 218 CCGG C^CGG
Ksp632I 133 CTCTTC CTCTTC(1/4)
MaeI 188 CTAG C^TAG
MaeII 212 ACGT A^CGT
MamI 72 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 72 GATC ^GATC
MslI 279 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 218 CCGG C^CGG
MvaI 225 CCAGG CC^[AT]GG
NciI 218 CCGGG CC^[GC]GG
NdeII 72 GATC ^GATC
PflFI 103 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 284 TCATGA T^CATGA
Sau3AI 72 GATC ^GATC
SfaNI 278 GCATC GCATC(5/9)
StuI 227 AGGCCT AGG^CCT
StyI 58 CCAAGG C^C[AT][AT]GG
Tth111I 103 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
ApaI 221 GGGCCC GGGCC^C
263 GGGCCC
AvaII 62 GGTCC G^G[AT]CC
116 GGTCC
BanII 221 GGGCCC G[GA]GC[CT]^C
263 GGGCCC
BsiYI 267 CCTGATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
303 CCTGTTACTGG
BslI 267 CCTGATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
303 CCTGTTACTGG
BsmFI 191 GGGAC GGGAC(10/14)
117 GTCCC
BsrI 143 ACTGG ACTGG(1/-1)
309 ACTGG
BssKI 218 CCGGG ^CC[ACGT]GG
225 CCAGG
DraII 220 GGGGCCC [GA]G^G[ACGT]CC[CT]
263 GGGCCCT
EcoO109I 220 GGGGCCC [GA]G^G[ACGT]CC[CT]
263 GGGCCCT
HinfI 270 GATTC G^A[ACGT]TC
296 GATTC
MboII 198 GAAGA GAAGA(8/7)
134 TCTTC
MwoI 149 GCTGTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
235 GCCTCCACCGC
ScrFI 218 CCGGG CC^[ACGT]GG
225 CCAGG
TfiI 270 GATTC G^A[AT]TC
296 GATTC
Tsp45I 214 GTGAC ^GT[GC]AC
325 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
BmyI BsaJI Bsp1286I BstF5I Cfr13I
FokI HaeIII MaeIII MnlI NlaIII
NlaIV Sau96I