Restriction map for
pFN21AE4432
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tru9I Tth111I
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 145 GACGTC GACGT^C
AccI 696 GTCTAC GT^[AC][GT]AC
AcyI 145 GACGTC G[GA]^CG[CT]C
AfaI 1302 GTAC GT^AC
AflIII 1235 ACATGT A^C[GA][CT]GT
AlwNI 434 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1053 GGGCCC GGGCC^C
AspHI 172 GAGCAC G[AT]GC[AT]^C
BamHI 1016 GGATCC G^GATCC
BanI 1339 GGCACC G^G[CT][GA]CC
BbsI 858 GAAGAC GAAGAC(2/6)
BciVI 480 GTATCC GTATCC(6/5)
BfaI 394 CTAG C^TAG
BpmI 1213 CTGGAG CTGGAG(16/14)
BpuAI 858 GAAGAC GAAGAC(2/6)
BsaHI 145 GACGTC G[GA]^CG[CT]C
BsaI 926 GAGACC GGTCTC(1/5)
BsaWI 632 ACCGGA [AT]^CCGG[AT]
BsiHKAI 172 GAGCAC G[AT]GC[AT]^C
BsmFI 430 GGGAC GGGAC(10/14)
BsmI 591 GAATGC GAATGC(1/-1)
BspDI 1294 ATCGAT AT^CGAT
BspLU11I 1235 ACATGT A^CATGT
BspMI 1097 ACCTGC ACCTGC(4/8)
BsrDI 420 CATTGC GCAATG(2/0)
BsrFI 811 ACCGGC [GA]^CCGG[CT]
BstXI 863 CCACGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 811 ACCGGC [GA]^CCGG[CT]
ClaI 1294 ATCGAT AT^CGAT
EarI 1159 CTCTTC CTCTTC(1/4)
HgaI 585 GACGC GACGC(5/10)
Hin1I 145 GACGTC G[GA]^CG[CT]C
HinfI 320 GATTC G^A[ACGT]TC
Ksp632I 1159 CTCTTC CTCTTC(1/4)
MaeI 394 CTAG C^TAG
MslI 1065 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1235 ACATGT [GA]CATG^[CT]
PflMI 837 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 861 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 892 CTGCAG CTGCA^G
RsaI 1302 GTAC GT^AC
SapI 1158 GCTCTTC GCTCTTC(1/4)
SmlI 574 CTCAAG C^T[CT][GA]AG
StuI 1205 AGGCCT AGG^CCT
TfiI 320 GATTC G^A[AT]TC
Tsp509I 559 AATT ^AATT
Van91I 837 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 393 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanII 1053 GGGCCC G[GA]GC[CT]^C
1123 GGGCTC
BglI 32 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
95 GCCCCGAGGGC
BsmAI 926 GAGAC GTCTC(1/5)
1089 GTCTC
BstYI 338 AGATCC [GA]^GATC[CT]
1016 GGATCC
CfoI 77 GCGC GCG^C
729 GCGC
DraII 1052 GGGGCCC [GA]G^G[ACGT]CC[CT]
1053 GGGCCCC
EcoO109I 1052 GGGGCCC [GA]G^G[ACGT]CC[CT]
1053 GGGCCCC
HhaI 77 GCGC GCG^C
729 GCGC
HinP1I 77 GCGC G^CGC
729 GCGC
HphI 216 GGTGA GGTGA(8/7)
17 TCACC
MaeII 146 ACGT A^CGT
905 ACGT
MaeIII 386 GTGAC ^GT[ACGT]AC
413 GTCAC
MboII 858 GAAGA GAAGA(8/7)
1160 TCTTC
MflI 338 AGATCC [GA]^GATC[CT]
1016 GGATCC
PvuII 70 CAGCTG CAG^CTG
688 CAGCTG
SfcI 22 CTACAG C^T[GA][CT]AG
892 CTGCAG
SmaI 185 CCCGGG CCC^GGG
212 CCCGGG
Tsp45I 386 GTGAC ^GT[GC]AC
413 GTCAC
XcmI 899 CCAAGAACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1061 CCAGCATCCAGGTGG
XhoII 338 AGATCC [GA]^GATC[CT]
1016 GGATCC
XmaCI 185 CCCGGG C^CCGGG
212 CCCGGG
XmaI 185 CCCGGG C^CCGGG
212 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BalI BbvI BcnI BmyI BsaJI
BseRI BsgI BsiYI BslI BsoBI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII ItaI
MboI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RcaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I