Restriction map for
pFN21AE6710
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# NO CUT ENZYMES ( 91 enzymes) #
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Acc65I AclI AcsI AflII AflIII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbvCI
BciVI BclI BfrI BlnI BsiWI
BspDI BspHI BspLU11I BsrDI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
KspI MfeI MluI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 442 GACGTC GACGT^C
AccI 1861 GTCTAC GT^[AC][GT]AC
AccIII 762 TCCGGA T^CCGGA
Aor51HI 223 AGCGCT AGC^GCT
AviII 448 TGCGCA TGC^GCA
BalI 1268 TGGCCA TGG^CCA
BcnI 16 CCGGG CC^[GC]GG
BfaI 245 CTAG C^TAG
BglII 119 AGATCT A^GATCT
BsaBI 1626 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 762 TCCGGA T^CCGGA
BsgI 580 GTGCAG GTGCAG(16/14)
BsmI 1426 GAATGC GAATGC(1/-1)
Bsp1407I 1919 TGTACA T^GTACA
BspEI 762 TCCGGA T^CCGGA
BsrBI 42 GAGCGG CCGCTC(-3/-3)
BsrFI 311 ACCGGC [GA]^CCGG[CT]
BsrGI 1919 TGTACA T^GTACA
BssHII 356 GCGCGC G^CGCGC
BssSI 1975 CACGAG CACGAG(-5/-1)
BstAPI 900 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1020 GGTCACC G^GT[ACGT]ACC
BstPI 1020 GGTCACC G^GT[ACGT]ACC
Cfr10I 311 ACCGGC [GA]^CCGG[CT]
DraIII 1926 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1852 GACAATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 223 AGCGCT AGC^GCT
EcoO65I 1020 GGTCACC G^GT[ACGT]ACC
FspI 448 TGCGCA TGC^GCA
HincII 1279 GTCAAC GT[CT]^[GA]AC
HindII 1279 GTCAAC GT[CT]^[GA]AC
MaeI 245 CTAG C^TAG
MamI 1626 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1268 TGGCCA TGG^CCA
MroI 762 TCCGGA T^CCGGA
MscI 1268 TGGCCA TGG^CCA
MseI 1139 TTAA T^TAA
NciI 16 CCGGG CC^[GC]GG
NheI 244 GCTAGC G^CTAGC
PpuMI 950 GGGTCCT [GA]G^G[AT]CC[CT]
SmlI 1705 CTCAAG C^T[CT][GA]AG
SspBI 1919 TGTACA T^GTACA
StuI 1601 AGGCCT AGG^CCT
Tru9I 1139 TTAA T^TAA
Tsp45I 1021 GTCAC ^GT[GC]AC
XcmI 710 CCAAAATCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AocI 954 CCTGAGG CC^T[ACGT]AGG
1941 CCTGAGG
ApaI 478 GGGCCC GGGCC^C
545 GGGCCC
AvaI 376 CCCGAG C^[CT]CG[GA]G
462 CTCGGG
BbrPI 613 CACGTG CAC^GTG
1929 CACGTG
BbsI 2056 GAAGAC GAAGAC(2/6)
730 GTCTTC
BcgI 1637 GCAGCCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1883 GCATCGCCATCG
BpuAI 2056 GAAGAC GAAGAC(2/6)
730 GTCTTC
BsaI 930 GAGACC GGTCTC(1/5)
2017 GAGACC
BsaWI 506 ACCGGA [AT]^CCGG[AT]
762 TCCGGA
BseRI 630 CTCCTC GAGGAG(10/8)
1735 CTCCTC
BsiEI 36 CGGCCG CG[GA][CT]^CG
313 CGGCCG
BsmBI 594 CGTCTC CGTCTC(1/5)
1305 CGTCTC
BsoBI 376 CCCGAG C^[CT]CG[GA]G
462 CTCGGG
BstXI 1164 CCAGCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1259 CCATTCGGATGG
BstYI 119 AGATCT [GA]^GATC[CT]
416 AGATCC
Bsu36I 954 CCTGAGG CC^T[ACGT]AGG
1941 CCTGAGG
EagI 36 CGGCCG C^GGCCG
313 CGGCCG
EclXI 36 CGGCCG C^GGCCG
313 CGGCCG
Eco52I 36 CGGCCG C^GGCCG
313 CGGCCG
Eco81I 954 CCTGAGG CC^T[ACGT]AGG
1941 CCTGAGG
EcoT14I 887 CCATGG C^C[AT][AT]GG
1652 CCATGG
MflI 119 AGATCT [GA]^GATC[CT]
416 AGATCC
NcoI 887 CCATGG C^CATGG
1652 CCATGG
PflMI 1450 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1925 CCACCACGTGG
PmaCI 613 CACGTG CAC^GTG
1929 CACGTG
PmlI 613 CACGTG CAC^GTG
1929 CACGTG
PvuII 1000 CAGCTG CAG^CTG
1165 CAGCTG
SexAI 176 ACCTGGT A^CC[AT]GGT
1283 ACCTGGT
StyI 887 CCATGG C^C[AT][AT]GG
1652 CCATGG
Van91I 1450 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1925 CCACCACGTGG
XhoII 119 AGATCT [GA]^GATC[CT]
416 AGATCC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbeI BbvI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NarI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
TaqI TfiI TseI TspRI TthHB8I