Restriction map for
pFN21AE5121
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BanII BbeI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaBI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspHI BspLU11I
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MslI MunI NarI NciI
NcoI NdeI NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI TspRI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 149 TCCGGA T^CCGGA
AcsI 211 AAATTC [GA]^AATT[CT]
AflIII 262 ACACGT A^C[GA][CT]GT
AlwI 138 GATCC GGATC(4/5)
AocI 204 CCTCAGG CC^T[ACGT]AGG
ApoI 211 AAATTC [GA]^AATT[CT]
AvaI 17 CTCGAG C^[CT]CG[GA]G
AvaII 243 GGACC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BbrPI 263 CACGTG CAC^GTG
BglII 21 AGATCT A^GATCT
BsaAI 263 CACGTG [CT]AC^GT[GA]
BsaJI 239 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 149 TCCGGA [AT]^CCGG[AT]
BseAI 149 TCCGGA T^CCGGA
BsiYI 69 CCTGAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 69 CCTGAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 84 GTCCC GGGAC(10/14)
BsoBI 17 CTCGAG C^[CT]CG[GA]G
BspEI 149 TCCGGA T^CCGGA
BspMI 245 ACCTGC ACCTGC(4/8)
BsrBI 331 GAGCGG CCGCTC(-3/-3)
BsrDI 159 CATTGC GCAATG(2/0)
BsrFI 278 GCCGGC [GA]^CCGG[CT]
BssKI 240 CCTGG ^CC[ACGT]GG
BssSI 292 CACGAG CACGAG(-5/-1)
BstNI 240 CCTGG CC^[AT]GG
Bsu36I 204 CCTCAGG CC^T[ACGT]AGG
Cac8I 278 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 278 GCCGGC [GA]^CCGG[CT]
Cfr13I 243 GGACC G^G[ACGT]CC
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eco81I 204 CCTCAGG CC^T[ACGT]AGG
EcoRII 240 CCTGG ^CC[AT]GG
HgaI 95 GCGTC GACGC(5/10)
MaeII 264 ACGT A^CGT
MaeIII 106 GTGAC ^GT[ACGT]AC
MluNI 2 TGGCCA TGG^CCA
MroI 149 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
MseI 182 TTAA T^TAA
MvaI 240 CCTGG CC^[AT]GG
NaeI 278 GCCGGC GCC^GGC
NgoMIV 278 GCCGGC G^CCGGC
NlaIII 270 CATG CATG^
PaeR7I 17 CTCGAG C^TCGAG
PmaCI 263 CACGTG CAC^GTG
PmlI 263 CACGTG CAC^GTG
Sau96I 243 GGACC G^G[ACGT]CC
ScrFI 240 CCTGG CC^[ACGT]GG
SmlI 17 CTCGAG C^T[CT][GA]AG
TaqI 18 TCGA T^CGA
Tru9I 182 TTAA T^TAA
Tsp45I 106 GTGAC ^GT[GC]AC
Tsp509I 212 AATT ^AATT
TthHB8I 18 TCGA T^CGA
XhoI 17 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BbsI 258 GAAGAC GAAGAC(2/6)
306 GAAGAC
BpuAI 258 GAAGAC GAAGAC(2/6)
306 GAAGAC
BseRI 43 GAGGAG GAGGAG(10/8)
295 GAGGAG
BstYI 21 AGATCT [GA]^GATC[CT]
137 AGATCC
CfoI 93 GCGC GCG^C
273 GCGC
FauI 342 CCCGC CCCGC(4/6)
29 GCGGG
HapII 150 CCGG C^CGG
279 CCGG
HhaI 93 GCGC GCG^C
273 GCGC
HinP1I 93 GCGC G^CGC
273 GCGC
HinfI 143 GAGTC G^A[ACGT]TC
189 GACTC
HpaII 150 CCGG C^CGG
279 CCGG
MflI 21 AGATCT [GA]^GATC[CT]
137 AGATCC
MspI 150 CCGG C^CGG
279 CCGG
PleI 189 GACTC GAGTC(4/5)
143 GAGTC
PvuII 58 CAGCTG CAG^CTG
88 CAGCTG
XhoII 21 AGATCT [GA]^GATC[CT]
137 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BstUI DdeI DpnII Fnu4HI HaeIII
ItaI MboI MboII MnlI MspA1I
MvnI MwoI NdeII RsaI Sau3AI
TseI