Restriction map for
pFN21AE1406
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AccIII AclI AcsI AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI AspEI
AviII AvrII BbvCI BciVI BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI BspDI
BspEI BspLU11I BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI MfeI MluI MroI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspI StuI SwaI TfiI
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 100 GACGTC GACGT^C
Acc65I 1515 GGTACC G^GTACC
AflII 562 CTTAAG C^TTAAG
AflIII 218 ACACGT A^C[GA][CT]GT
ApaI 811 GGGCCC GGGCC^C
Asp700I 1413 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1515 GGTACC G^GTACC
AspHI 1426 GAGCAC G[AT]GC[AT]^C
AspI 1222 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 797 TGGCCA TGG^CCA
BamHI 632 GGATCC G^GATCC
BbeI 1677 GGCGCC GGCGC^C
BbrPI 412 CACGTG CAC^GTG
BcgI 660 CGAGGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 308 TGATCA T^GATCA
BfrI 562 CTTAAG C^TTAAG
BglI 1015 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1169 AGATCT A^GATCT
BsaBI 642 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1088 CGGTCG CG[GA][CT]^CG
BsiHKAI 1426 GAGCAC G[AT]GC[AT]^C
Bsp1407I 749 TGTACA T^GTACA
BspHI 764 TCATGA T^CATGA
BsrFI 1660 ACCGGC [GA]^CCGG[CT]
BsrGI 749 TGTACA T^GTACA
BssSI 36 CACGAG CACGAG(-5/-1)
Cfr10I 1660 ACCGGC [GA]^CCGG[CT]
FbaI 308 TGATCA T^GATCA
HaeII 1677 GGCGCC [GA]GCGC^[CT]
HinfI 47 GACTC G^A[ACGT]TC
KasI 1677 GGCGCC G^GCGCC
KpnI 1515 GGTACC GGTAC^C
KspI 1408 CCGCGG CCGC^GG
MamI 642 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 797 TGGCCA TGG^CCA
MscI 797 TGGCCA TGG^CCA
NarI 1677 GGCGCC GG^CGCC
NspI 1490 GCATGT [GA]CATG^[CT]
PflFI 1222 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 47 GACTC GAGTC(4/5)
PmaCI 412 CACGTG CAC^GTG
PmlI 412 CACGTG CAC^GTG
PshAI 1086 GACGGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 226 CTGCAG CTGCA^G
PvuII 377 CAGCTG CAG^CTG
RcaI 764 TCATGA T^CATGA
SacII 1408 CCGCGG CCGC^GG
SfoI 1677 GGCGCC GGC^GCC
SmlI 562 CTTAAG C^T[CT][GA]AG
SpeI 1329 ACTAGT A^CTAGT
SspBI 749 TGTACA T^GTACA
Tsp509I 484 AATT ^AATT
Tth111I 1222 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1413 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 1409 CGCG CG^CG
1441 CGCG
AvaI 658 CCCGAG C^[CT]CG[GA]G
1054 CCCGAG
BfaI 817 CTAG C^TAG
1330 CTAG
BsaAI 219 CACGTA [CT]AC^GT[GA]
412 CACGTG
BsgI 466 GTGCAG GTGCAG(16/14)
776 GTGCAG
BsoBI 658 CCCGAG C^[CT]CG[GA]G
1054 CCCGAG
BspMI 224 ACCTGC ACCTGC(4/8)
978 GCAGGT
BsrDI 723 CATTGC GCAATG(2/0)
834 CATTGC
BstUI 1409 CGCG CG^CG
1441 CGCG
BstYI 632 GGATCC [GA]^GATC[CT]
1169 AGATCT
DrdI 472 GACAAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
973 GACTTGCAGGTC
EarI 18 GAAGAG CTCTTC(1/4)
685 GAAGAG
HgaI 479 GCGTC GACGC(5/10)
1442 GCGTC
Ksp632I 18 GAAGAG CTCTTC(1/4)
685 GAAGAG
MaeI 817 CTAG C^TAG
1330 CTAG
MflI 632 GGATCC [GA]^GATC[CT]
1169 AGATCT
MvnI 1409 CGCG CG^CG
1441 CGCG
PpuMI 654 GGGTCCC [GA]G^G[AT]CC[CT]
1235 GGGACCC
SapI 18 GAAGAGC GCTCTTC(1/4)
685 GAAGAGC
SfcI 226 CTGCAG C^T[GA][CT]AG
1005 CTACAG
TaqI 359 TCGA T^CGA
1301 TCGA
TthHB8I 359 TCGA T^CGA
1301 TCGA
XhoII 632 GGATCC [GA]^GATC[CT]
1169 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI Tru9I TseI Tsp45I TspRI
XcmI