Restriction map for
pFN21AE4584
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# NO CUT ENZYMES ( 80 enzymes) #
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AccI AccIII AclI AflII AgeI
AocI Aor51HI AscI AseI AsnI
Asp700I AviII AvrII BamHI BfaI
BfrI BlnI BseAI BsiWI BspDI
BspEI BspLU11I Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eco47III Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HindIII
HpaI MaeI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SalI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI SwaI Tru9I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 553 GACGTC GACGT^C
Acc65I 1649 GGTACC G^GTACC
AflIII 863 ACGTGT A^C[GA][CT]GT
AhdI 862 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1649 GGTACC G^GTACC
AspEI 862 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 900 CTCGGG C^[CT]CG[GA]G
BbvCI 998 CCTCAGC CCTCAGC(-5/-2)
BciVI 2552 GTATCC GTATCC(6/5)
BclI 566 TGATCA T^GATCA
BglI 310 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 662 AGATCT A^GATCT
BlpI 1849 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1849 GCTAAGC GC^T[ACGT]AGC
BsaBI 1977 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1918 GAGACC GGTCTC(1/5)
BsmI 1074 GAATGC GAATGC(1/-1)
BsoBI 900 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2345 TGTACA T^GTACA
BspMI 2545 ACCTGC ACCTGC(4/8)
BsrDI 2516 GCAATG GCAATG(2/0)
BsrGI 2345 TGTACA T^GTACA
BssHII 1411 GCGCGC G^CGCGC
BssSI 2203 CTCGTG CACGAG(-5/-1)
BstAPI 189 GCAGGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1849 GCTAAGC GC^T[ACGT]AGC
DrdI 403 GACATCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2291 CGGCCG C^GGCCG
Eam1105I 862 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2291 CGGCCG C^GGCCG
Eco52I 2291 CGGCCG C^GGCCG
EcoRI 1507 GAATTC G^AATTC
FbaI 566 TGATCA T^GATCA
HincII 1165 GTCAAC GT[CT]^[GA]AC
HindII 1165 GTCAAC GT[CT]^[GA]AC
KpnI 1649 GGTACC GGTAC^C
MamI 1977 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1777 GCCGGC GCC^GGC
NcoI 1766 CCATGG C^CATGG
NgoMIV 1777 GCCGGC G^CCGGC
SbfI 258 CCTGCAGG CCTGCA^GG
ScaI 62 AGTACT AGT^ACT
SexAI 1544 ACCTGGT A^CC[AT]GGT
SfiI 309 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 258 CCTGCAGG CCTGCA^GG
SspBI 2345 TGTACA T^GTACA
TfiI 1658 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 17 enzymes) #
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Alw44I 1530 GTGCAC G^TGCAC
2334 GTGCAC
ApaLI 1530 GTGCAC G^TGCAC
2334 GTGCAC
BbeI 242 GGCGCC GGCGC^C
1676 GGCGCC
BsaWI 1652 ACCGGA [AT]^CCGG[AT]
2279 ACCGGA
BsmBI 694 GAGACG CGTCTC(1/5)
2104 GAGACG
BsrBI 811 GAGCGG CCGCTC(-3/-3)
1876 GAGCGG
HgaI 696 GACGC GACGC(5/10)
350 GCGTC
KasI 242 GGCGCC G^GCGCC
1676 GGCGCC
NarI 242 GGCGCC GG^CGCC
1676 GGCGCC
PflMI 1971 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2168 CCAAGCCCTGG
SacI 496 GAGCTC GAGCT^C
2296 GAGCTC
SapI 1734 GAAGAGC GCTCTTC(1/4)
2076 GAAGAGC
SfaNI 206 GCATC GCATC(5/9)
1559 GCATC
SfoI 242 GGCGCC GGC^GCC
1676 GGCGCC
StuI 776 AGGCCT AGG^CCT
1757 AGGCCT
Tsp45I 1771 GTGAC ^GT[GC]AC
2231 GTGAC
Van91I 1971 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2168 CCAAGCCCTGG
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaII BalI BanI
BanII BbrPI BbsI BbvI BcgI
BcnI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PmaCI
PmlI PpuMI PstI RcaI RsaI
SacII Sau3AI Sau96I ScrFI SfcI
StyI TaqI TseI Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII