Restriction map for
pFN21AE3595
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbsI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmFI BsoBI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AfaI 498 GTAC GT^AC
AlwNI 488 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 35 TGGCCA TGG^CCA
BamHI 632 GGATCC G^GATCC
BanII 364 GAGCTC G[GA]GC[CT]^C
BbvCI 19 GCTGAGG CCTCAGC(-5/-2)
BcgI 420 CGAGAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 255 CCGGG CC^[GC]GG
BpmI 199 CTGGAG CTGGAG(16/14)
BsaBI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 47 CCAAGG C^C[ACGT][ACGT]GG
BseRI 343 GAGGAG GAGGAG(10/8)
BsgI 671 GTGCAG GTGCAG(16/14)
BsiYI 816 CCTCTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 816 CCTCTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 193 GAATGC GAATGC(1/-1)
BspDI 277 ATCGAT AT^CGAT
BspMI 655 ACCTGC ACCTGC(4/8)
BssSI 367 CTCGTG CACGAG(-5/-1)
BstAPI 513 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 277 ATCGAT AT^CGAT
DraII 812 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 35 TGGCCA [CT]^GGCC[GA]
EarI 1057 CTCTTC CTCTTC(1/4)
EcoNI 816 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 812 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 47 CCAAGG C^C[AT][AT]GG
EcoT22I 727 ATGCAT ATGCA^T
FauI 895 GCGGG CCCGC(4/6)
HapII 255 CCGG C^CGG
HpaII 255 CCGG C^CGG
Ksp632I 1057 CTCTTC CTCTTC(1/4)
MamI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 35 TGGCCA TGG^CCA
MscI 35 TGGCCA TGG^CCA
MseI 443 TTAA T^TAA
MslI 169 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 255 CCGG C^CGG
NciI 255 CCGGG CC^[GC]GG
NdeI 756 CATATG CA^TATG
NsiI 727 ATGCAT ATGCA^T
PpuMI 812 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 498 GTAC GT^AC
SacI 364 GAGCTC GAGCT^C
SmlI 1042 CTCAAG C^T[CT][GA]AG
StyI 47 CCAAGG C^C[AT][AT]GG
TfiI 720 GAATC G^A[AT]TC
Tru9I 443 TTAA T^TAA
XcmI 260 CCATGACCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AvaII 44 GGTCC G^G[AT]CC
813 GGACC
BfaI 55 CTAG C^TAG
506 CTAG
BstYI 613 AGATCC [GA]^GATC[CT]
632 GGATCC
Cac8I 408 GCAAGC GC[ACGT]^[ACGT]GC
412 GCCTGC
MaeI 55 CTAG C^TAG
506 CTAG
MflI 613 AGATCC [GA]^GATC[CT]
632 GGATCC
Tsp45I 652 GTCAC ^GT[GC]AC
962 GTCAC
XhoII 613 AGATCC [GA]^GATC[CT]
632 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BbvI BmyI BsiHKAI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp509I TspRI TthHB8I