Restriction map for
pFN21AE1574
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BbeI BbrPI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI BspEI
BspHI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KspI
MamI MluI MroI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI TfiI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 203 GGTACC G^GTACC
AccI 1606 GTCTAC GT^[AC][GT]AC
AflIII 140 ACGTGT A^C[GA][CT]GT
Alw44I 685 GTGCAC G^TGCAC
AocI 1915 CCTCAGG CC^T[ACGT]AGG
ApaLI 685 GTGCAC G^TGCAC
Asp700I 177 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 203 GGTACC G^GTACC
AspHI 685 GTGCAC G[AT]GC[AT]^C
BalI 1856 TGGCCA TGG^CCA
BamHI 340 GGATCC G^GATCC
BanI 203 GGTACC G^G[CT][GA]CC
BbsI 1615 GTCTTC GAAGAC(2/6)
BbvCI 381 GCTGAGG CCTCAGC(-5/-2)
BcgI 660 CGACCACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 884 GTATCC GTATCC(6/5)
BglI 125 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1955 AGATCT A^GATCT
BpuAI 1615 GTCTTC GAAGAC(2/6)
BsgI 151 GTGCAG GTGCAG(16/14)
BsiHKAI 685 GTGCAC G[AT]GC[AT]^C
Bsp1407I 359 TGTACA T^GTACA
BspDI 694 ATCGAT AT^CGAT
BspMI 914 ACCTGC ACCTGC(4/8)
BsrBI 1222 GAGCGG CCGCTC(-3/-3)
BsrDI 482 GCAATG GCAATG(2/0)
BsrFI 1192 GCCGGC [GA]^CCGG[CT]
BsrGI 359 TGTACA T^GTACA
BstAPI 1939 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 674 CCAACGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1915 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1192 GCCGGC [GA]^CCGG[CT]
ClaI 694 ATCGAT AT^CGAT
CpoI 365 CGGACCG CG^G[AT]CCG
DrdI 469 GACTCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1350 CCATGG C^C[GA][CT]GG
EarI 7 CTCTTC CTCTTC(1/4)
Eco81I 1915 CCTCAGG CC^T[ACGT]AGG
HaeII 3 GGCGCT [GA]GCGC^[CT]
KpnI 203 GGTACC GGTAC^C
Ksp632I 7 CTCTTC CTCTTC(1/4)
MaeII 140 ACGT A^CGT
MfeI 1277 CAATTG C^AATTG
MluNI 1856 TGGCCA TGG^CCA
MscI 1856 TGGCCA TGG^CCA
MseI 1319 TTAA T^TAA
MunI 1277 CAATTG C^AATTG
NaeI 1192 GCCGGC GCC^GGC
NcoI 1350 CCATGG C^CATGG
NdeI 520 CATATG CA^TATG
NgoMIV 1192 GCCGGC G^CCGGC
PflMI 519 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 365 CGGACCG CG^G[AT]CCG
SapI 6 GCTCTTC GCTCTTC(1/4)
ScaI 179 AGTACT AGT^ACT
SmlI 1951 CTTGAG C^T[CT][GA]AG
SphI 1547 GCATGC GCATG^C
SspBI 359 TGTACA T^GTACA
Tru9I 1319 TTAA T^TAA
Tsp45I 1781 GTGAC ^GT[GC]AC
Van91I 519 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 177 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AccII 57 CGCG CG^CG
1437 CGCG
BstUI 57 CGCG CG^CG
1437 CGCG
CfoI 4 GCGC GCG^C
1948 GCGC
EaeI 816 CGGCCA [CT]^GGCC[GA]
1856 TGGCCA
EcoRI 588 GAATTC G^AATTC
1248 GAATTC
HhaI 4 GCGC GCG^C
1948 GCGC
HinP1I 4 GCGC G^CGC
1948 GCGC
MslI 573 CACCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1714 CACATTGGTG
MvnI 57 CGCG CG^CG
1437 CGCG
NspI 1025 ACATGC [GA]CATG^[CT]
1547 GCATGC
TspRI 199 CAGTG CA[GC]TG[ACGT][ACGT]^
1001 CACTG
XcmI 609 CCACTATCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1859 CCACAAATGGCGTGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TseI Tsp509I
TthHB8I XhoII