Restriction map for
pFN21AE6304
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BstBI BstXI BstYI
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NarI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 807 GTATAC GT^[AC][GT]AC
AcsI 464 GAATTT [GA]^AATT[CT]
AlwI 836 GGATC GGATC(4/5)
AlwNI 1109 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 464 GAATTT [GA]^AATT[CT]
AspHI 1218 GAGCTC G[AT]GC[AT]^C
AvaII 1136 GGACC G^G[AT]CC
BsaAI 1198 TACGTG [CT]AC^GT[GA]
BsaI 297 GAGACC GGTCTC(1/5)
BseRI 24 CTCCTC GAGGAG(10/8)
BsiEI 1291 CGGCCG CG[GA][CT]^CG
BsiHKAI 1218 GAGCTC G[AT]GC[AT]^C
BsrBI 1296 GAGCGG CCGCTC(-3/-3)
BsrDI 843 CATTGC GCAATG(2/0)
BsrFI 1058 GCCGGC [GA]^CCGG[CT]
BssSI 1093 CTCGTG CACGAG(-5/-1)
Bst1107I 807 GTATAC GTA^TAC
BstAPI 595 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1038 GGTCACC G^GT[ACGT]ACC
BstPI 1038 GGTCACC G^GT[ACGT]ACC
BstZ17I 807 GTATAC GTA^TAC
Cfr10I 1058 GCCGGC [GA]^CCGG[CT]
DsaI 908 CCACGG C^C[GA][CT]GG
EagI 1291 CGGCCG C^GGCCG
EarI 790 CTCTTC CTCTTC(1/4)
EclXI 1291 CGGCCG C^GGCCG
Eco52I 1291 CGGCCG C^GGCCG
EcoO65I 1038 GGTCACC G^GT[ACGT]ACC
EcoT14I 755 CCTTGG C^C[AT][AT]GG
HaeII 1068 GGCGCT [GA]GCGC^[CT]
Ksp632I 790 CTCTTC CTCTTC(1/4)
MaeII 1199 ACGT A^CGT
NaeI 1058 GCCGGC GCC^GGC
NgoMIV 1058 GCCGGC G^CCGGC
NheI 702 GCTAGC G^CTAGC
NspI 173 GCATGT [GA]CATG^[CT]
SacI 1218 GAGCTC GAGCT^C
SapI 789 GCTCTTC GCTCTTC(1/4)
StuI 588 AGGCCT AGG^CCT
StyI 755 CCTTGG C^C[AT][AT]GG
TaqI 920 TCGA T^CGA
TthHB8I 920 TCGA T^CGA
XcmI 746 CCATTCTGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 1063 CGCG CG^CG
1223 CGCG
AfaI 512 GTAC GT^AC
1170 GTAC
ApaI 543 GGGCCC GGGCC^C
1315 GGGCCC
AvaI 1013 CCCGGG C^[CT]CG[GA]G
1047 CCCGGG
BanI 633 GGTGCC G^G[CT][GA]CC
1250 GGCACC
BbsI 382 GAAGAC GAAGAC(2/6)
1003 GAAGAC
BpuAI 382 GAAGAC GAAGAC(2/6)
1003 GAAGAC
BsmFI 583 GGGAC GGGAC(10/14)
1307 GGGAC
BsoBI 1013 CCCGGG C^[CT]CG[GA]G
1047 CCCGGG
BsrI 554 ACTGG ACTGG(1/-1)
232 CCAGT
BstF5I 596 CATCC GGATG(2/0)
879 CATCC
BstUI 1063 CGCG CG^CG
1223 CGCG
DpnII 690 GATC ^GATC
837 GATC
FauI 882 CCCGC CCCGC(4/6)
1304 GCGGG
FokI 596 CATCC GGATG(9/13)
879 CATCC
HgaI 1204 GACGC GACGC(5/10)
1309 GACGC
MaeIII 181 GTCAC ^GT[ACGT]AC
1039 GTCAC
MboI 690 GATC ^GATC
837 GATC
MvnI 1063 CGCG CG^CG
1223 CGCG
NdeII 690 GATC ^GATC
837 GATC
PflMI 848 CCAACACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1077 CCACTGCCTGG
PstI 518 CTGCAG CTGCA^G
1239 CTGCAG
PvuII 187 CAGCTG CAG^CTG
521 CAGCTG
RsaI 512 GTAC GT^AC
1170 GTAC
Sau3AI 690 GATC ^GATC
837 GATC
SfcI 518 CTGCAG C^T[GA][CT]AG
1239 CTGCAG
SmaI 1013 CCCGGG CCC^GGG
1047 CCCGGG
TfiI 765 GAATC G^A[AT]TC
947 GAATC
Tsp45I 181 GTCAC ^GT[GC]AC
1039 GTCAC
Van91I 848 CCAACACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1077 CCACTGCCTGG
XmaCI 1013 CCCGGG C^CCGGG
1047 CCCGGG
XmaI 1013 CCCGGG C^CCGGG
1047 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BfaI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BspMI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI TseI Tsp509I TspRI