Restriction map for
pFN21AE5617
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoT22I
FseI FspI HpaI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI XbaI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 172 GTCTAC GT^[AC][GT]AC
AcsI 1300 GAATTC [GA]^AATT[CT]
AflIII 1082 ACATGT A^C[GA][CT]GT
AlwI 1440 GGATC GGATC(4/5)
ApaI 498 GGGCCC GGGCC^C
ApoI 1300 GAATTC [GA]^AATT[CT]
AspI 46 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 746 CTCGAG C^[CT]CG[GA]G
BbeI 55 GGCGCC GGCGC^C
BbsI 814 GAAGAC GAAGAC(2/6)
BclI 1092 TGATCA T^GATCA
BcnI 41 CCGGG CC^[GC]GG
BglI 289 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 814 GAAGAC GAAGAC(2/6)
BsgI 425 CTGCAC GTGCAG(16/14)
BsmBI 108 CGTCTC CGTCTC(1/5)
BsoBI 746 CTCGAG C^[CT]CG[GA]G
BspLU11I 1082 ACATGT A^CATGT
BsrDI 1311 CATTGC GCAATG(2/0)
BsrI 700 ACTGG ACTGG(1/-1)
BstAPI 343 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1149 GGTCACC G^GT[ACGT]ACC
BstPI 1149 GGTCACC G^GT[ACGT]ACC
EcoO65I 1149 GGTCACC G^GT[ACGT]ACC
EcoRI 1300 GAATTC G^AATTC
EcoRV 229 GATATC GAT^ATC
FauI 60 CCCGC CCCGC(4/6)
FbaI 1092 TGATCA T^GATCA
HaeII 55 GGCGCC [GA]GCGC^[CT]
HapII 41 CCGG C^CGG
HincII 1168 GTCAAC GT[CT]^[GA]AC
HindII 1168 GTCAAC GT[CT]^[GA]AC
HindIII 1073 AAGCTT A^AGCTT
HpaII 41 CCGG C^CGG
KasI 55 GGCGCC G^GCGCC
MaeII 657 ACGT A^CGT
MseI 332 TTAA T^TAA
MslI 1269 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 41 CCGG C^CGG
NarI 55 GGCGCC GG^CGCC
NciI 41 CCGGG CC^[GC]GG
NspI 1082 ACATGT [GA]CATG^[CT]
PaeR7I 746 CTCGAG C^TCGAG
PflFI 46 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1543 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1534 GAGCTC GAGCT^C
SfoI 55 GGCGCC GGC^GCC
SmlI 746 CTCGAG C^T[CT][GA]AG
TaqI 747 TCGA T^CGA
Tru9I 332 TTAA T^TAA
Tth111I 46 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 747 TCGA T^CGA
Van91I 1543 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 746 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 62 CGCG CG^CG
106 CGCG
AcyI 55 GGCGCC G[GA]^CG[CT]C
304 GACGCC
AocI 574 CCTGAGG CC^T[ACGT]AGG
1237 CCTGAGG
BanII 498 GGGCCC G[GA]GC[CT]^C
1534 GAGCTC
BsaHI 55 GGCGCC G[GA]^CG[CT]C
304 GACGCC
BspMI 1229 ACCTGC ACCTGC(4/8)
1495 ACCTGC
BstUI 62 CGCG CG^CG
106 CGCG
Bsu36I 574 CCTGAGG CC^T[ACGT]AGG
1237 CCTGAGG
DsaI 113 CCATGG C^C[GA][CT]GG
1154 CCATGG
Eco81I 574 CCTGAGG CC^T[ACGT]AGG
1237 CCTGAGG
HgaI 304 GACGC GACGC(5/10)
107 GCGTC
Hin1I 55 GGCGCC G[GA]^CG[CT]C
304 GACGCC
MspA1I 70 CCGCTG C[AC]G^C[GT]G
989 CAGCGG
MvnI 62 CGCG CG^CG
106 CGCG
NcoI 113 CCATGG C^CATGG
1154 CCATGG
PpuMI 544 GGGTCCC [GA]G^G[AT]CC[CT]
570 AGGACCT
SfcI 268 CTACAG C^T[GA][CT]AG
849 CTACAG
StuI 248 AGGCCT AGG^CCT
626 AGGCCT
Tsp509I 311 AATT ^AATT
1301 AATT
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp45I TspRI XcmI