Restriction map for
pFN21AE6718
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AflII AflIII
AgeI AhdI AocI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BanI BbeI BbrPI
BcgI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsgI BsiWI BsmBI
Bsp1407I BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 623 TCCGGA T^CCGGA
AclI 504 AACGTT AA^CGTT
AfaI 93 GTAC GT^AC
Alw44I 675 GTGCAC G^TGCAC
ApaI 1735 GGGCCC GGGCC^C
ApaLI 675 GTGCAC G^TGCAC
AvaI 911 CTCGAG C^[CT]CG[GA]G
BamHI 1788 GGATCC G^GATCC
BbsI 1483 GAAGAC GAAGAC(2/6)
BbvCI 316 CCTCAGC CCTCAGC(-5/-2)
BclI 1550 TGATCA T^GATCA
BfaI 1205 CTAG C^TAG
BpuAI 1483 GAAGAC GAAGAC(2/6)
BsaBI 654 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 623 TCCGGA [AT]^CCGG[AT]
BseAI 623 TCCGGA T^CCGGA
BseRI 1573 GAGGAG GAGGAG(10/8)
BsiEI 122 CGATCG CG[GA][CT]^CG
BsmI 541 GCATTC GAATGC(1/-1)
BsoBI 911 CTCGAG C^[CT]CG[GA]G
BspDI 124 ATCGAT AT^CGAT
BspEI 623 TCCGGA T^CCGGA
BspHI 950 TCATGA T^CATGA
BsrDI 261 CATTGC GCAATG(2/0)
ClaI 124 ATCGAT AT^CGAT
DraIII 1918 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1378 GACTGCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 717 GAAGAG CTCTTC(1/4)
EcoNI 66 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 769 GAATTC G^AATTC
FbaI 1550 TGATCA T^GATCA
Ksp632I 717 GAAGAG CTCTTC(1/4)
MaeI 1205 CTAG C^TAG
MamI 654 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 623 TCCGGA T^CCGGA
MslI 1227 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 1368 TCGCGA TCG^CGA
PaeR7I 911 CTCGAG C^TCGAG
PpuMI 290 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 504 AACGTT AA^CGTT
PvuI 122 CGATCG CGAT^CG
RcaI 950 TCATGA T^CATGA
RsaI 93 GTAC GT^AC
SacI 1600 GAGCTC GAGCT^C
SphI 744 GCATGC GCATG^C
XhoI 911 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 1336 GGCGTC G[GA]^CG[CT]C
1384 GGCGTC
BalI 1418 TGGCCA TGG^CCA
2024 TGGCCA
BsaHI 1336 GGCGTC G[GA]^CG[CT]C
1384 GGCGTC
BsmFI 34 GTCCC GGGAC(10/14)
292 GTCCC
BspMI 1654 ACCTGC ACCTGC(4/8)
112 GCAGGT
BstBI 58 TTCGAA TT^CGAA
991 TTCGAA
EcoT14I 1427 CCAAGG C^C[AT][AT]GG
1865 CCAAGG
FauI 1835 CCCGC CCCGC(4/6)
1635 GCGGG
HgaI 1337 GCGTC GACGC(5/10)
1385 GCGTC
Hin1I 1336 GGCGTC G[GA]^CG[CT]C
1384 GGCGTC
HincII 304 GTCAAC GT[CT]^[GA]AC
2005 GTTGAC
HindII 304 GTCAAC GT[CT]^[GA]AC
2005 GTTGAC
KspI 1280 CCGCGG CCGC^GG
1317 CCGCGG
MluNI 1418 TGGCCA TGG^CCA
2024 TGGCCA
MscI 1418 TGGCCA TGG^CCA
2024 TGGCCA
NspI 744 GCATGC [GA]CATG^[CT]
1694 ACATGC
NspV 58 TTCGAA TT^CGAA
991 TTCGAA
PflMI 1584 CCACGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1917 CCACACTGTGG
PvuII 163 CAGCTG CAG^CTG
1963 CAGCTG
SacII 1280 CCGCGG CCGC^GG
1317 CCGCGG
SfuI 58 TTCGAA TT^CGAA
991 TTCGAA
SmlI 911 CTCGAG C^T[CT][GA]AG
1530 CTTGAG
StyI 1427 CCAAGG C^C[AT][AT]GG
1865 CCAAGG
Tsp45I 1339 GTCAC ^GT[GC]AC
1828 GTCAC
Van91I 1584 CCACGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1917 CCACACTGTGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI Aor51HI ApoI AspHI AvaII
BanII BbvI BcnI BglI BglII
BmyI BpmI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco47III
EcoO109I EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII