Restriction map for
pFN21AE3256
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII Alw44I AocI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbsI BcgI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BseAI BsiWI BspDI
BspEI BspHI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI EarI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI Ksp632I
MaeI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 910 GTCGAC GT^[AC][GT]AC
AgeI 844 ACCGGT A^CCGGT
AhdI 1336 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1178 AGCGCT AGC^GCT
ApaI 811 GGGCCC GGGCC^C
AscI 534 GGCGCGCC GG^CGCGCC
AspEI 1336 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 865 GTGCTC G[AT]GC[AT]^C
BalI 404 TGGCCA TGG^CCA
BanII 811 GGGCCC G[GA]GC[CT]^C
BbrPI 490 CACGTG CAC^GTG
BciVI 1555 GGATAC GTATCC(6/5)
BglI 488 GCCACGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 316 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 316 GCTGAGC GC^T[ACGT]AGC
BsaWI 844 ACCGGT [AT]^CCGG[AT]
BsiHKAI 865 GTGCTC G[AT]GC[AT]^C
BsmBI 1545 CGTCTC CGTCTC(1/5)
BsmI 824 GAATGC GAATGC(1/-1)
Bsp1407I 1451 TGTACA T^GTACA
BspLU11I 1503 ACATGT A^CATGT
BsrDI 1001 GCAATG GCAATG(2/0)
BsrGI 1451 TGTACA T^GTACA
BstF5I 1428 GGATG GGATG(2/0)
BstXI 407 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 316 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1336 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1178 AGCGCT AGC^GCT
FokI 1428 GGATG GGATG(9/13)
HgaI 828 GCGTC GACGC(5/10)
HincII 910 GTCGAC GT[CT]^[GA]AC
HindII 910 GTCGAC GT[CT]^[GA]AC
HphI 1308 GGTGA GGTGA(8/7)
MboII 1673 TCTTC GAAGA(8/7)
MluNI 404 TGGCCA TGG^CCA
MscI 404 TGGCCA TGG^CCA
NaeI 1265 GCCGGC GCC^GGC
NgoMIV 1265 GCCGGC G^CCGGC
PflMI 1646 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 844 ACCGGT A^CCGGT
PmaCI 490 CACGTG CAC^GTG
PmlI 490 CACGTG CAC^GTG
PpuMI 1772 GGGACCC [GA]G^G[AT]CC[CT]
SalI 910 GTCGAC G^TCGAC
SmlI 1068 CTCAAG C^T[CT][GA]AG
SspBI 1451 TGTACA T^GTACA
TfiI 1494 GAATC G^A[AT]TC
Van91I 1646 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1277 CCATCGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 1448 ACGTGT A^C[GA][CT]GT
1503 ACATGT
AlwI 858 GATCC GGATC(4/5)
1482 GATCC
AlwNI 262 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
655 CAGCTGCTG
AvaI 450 CTCGGG C^[CT]CG[GA]G
1701 CTCGGG
BbvCI 433 GCTGAGG CCTCAGC(-5/-2)
1438 GCTGAGG
BsaI 1300 GAGACC GGTCTC(1/5)
847 GGTCTC
BseRI 1128 CTCCTC GAGGAG(10/8)
583 GAGGAG
BsgI 235 CTGCAC GTGCAG(16/14)
1786 CTGCAC
BsoBI 450 CTCGGG C^[CT]CG[GA]G
1701 CTCGGG
BspMI 341 ACCTGC ACCTGC(4/8)
551 ACCTGC
BsrBI 223 GAGCGG CCGCTC(-3/-3)
520 GAGCGG
BssSI 862 CTCGTG CACGAG(-5/-1)
868 CTCGTG
BstAPI 237 GCACGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
261 GCAGCTGCTGC
BstYI 857 AGATCC [GA]^GATC[CT]
1481 AGATCC
DpnII 858 GATC ^GATC
1482 GATC
DraIII 238 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1251 CACTGCGTG
DrdI 895 GACGAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1017 GACGGGTGGGTC
EagI 356 CGGCCG C^GGCCG
1762 CGGCCG
EclXI 356 CGGCCG C^GGCCG
1762 CGGCCG
Eco52I 356 CGGCCG C^GGCCG
1762 CGGCCG
KspI 807 CCGCGG CCGC^GG
1768 CCGCGG
MboI 858 GATC ^GATC
1482 GATC
MflI 857 AGATCC [GA]^GATC[CT]
1481 AGATCC
NcoI 1232 CCATGG C^CATGG
1304 CCATGG
NdeII 858 GATC ^GATC
1482 GATC
SacII 807 CCGCGG CCGC^GG
1768 CCGCGG
Sau3AI 858 GATC ^GATC
1482 GATC
StuI 437 AGGCCT AGG^CCT
1592 AGGCCT
XhoII 857 AGATCC [GA]^GATC[CT]
1481 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BbeI BbvI BcnI
BmyI BpmI BsaAI BsaHI BsaJI
BsiEI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
MaeII MaeIII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau96I ScrFI
SfaNI SfcI SfoI StyI TaqI
TseI Tsp45I TspRI TthHB8I