Restriction map for
pFN21AE3094
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HgaI HincII
HindII HindIII HinfI HpaI KpnI
KspI MaeI MamI MfeI MluI
MluNI MscI MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacII SalI ScaI
SexAI SfuI SmaI SmlI SnaBI
SpeI SspBI SspI StyI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 118 TCCGGA T^CCGGA
AcyI 784 GGCGCC G[GA]^CG[CT]C
AlwI 128 GGATC GGATC(4/5)
AlwNI 592 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 204 GGGCCC GGGCC^C
BanI 784 GGCGCC G^G[CT][GA]CC
BbeI 784 GGCGCC GGCGC^C
BcgI 193 GCAGGCATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 222 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 222 GCTGAGC GC^T[ACGT]AGC
BsaHI 784 GGCGCC G[GA]^CG[CT]C
BsaWI 118 TCCGGA [AT]^CCGG[AT]
BseAI 118 TCCGGA T^CCGGA
BsmFI 306 GTCCC GGGAC(10/14)
BspEI 118 TCCGGA T^CCGGA
BspMI 506 ACCTGC ACCTGC(4/8)
BsrBI 225 GAGCGG CCGCTC(-3/-3)
BsrI 87 CCAGT ACTGG(1/-1)
BstYI 128 GGATCT [GA]^GATC[CT]
CelII 222 GCTGAGC GC^T[ACGT]AGC
DraII 204 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoNI 717 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 204 GGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 227 GCGGG CCCGC(4/6)
Hin1I 784 GGCGCC G[GA]^CG[CT]C
KasI 784 GGCGCC G^GCGCC
MflI 128 GGATCT [GA]^GATC[CT]
MroI 118 TCCGGA T^CCGGA
MseI 707 TTAA T^TAA
NarI 784 GGCGCC GG^CGCC
PstI 508 CTGCAG CTGCA^G
SacI 167 GAGCTC GAGCT^C
SapI 246 GAAGAGC GCTCTTC(1/4)
SbfI 507 CCTGCAGG CCTGCA^GG
SfcI 508 CTGCAG C^T[GA][CT]AG
SfiI 465 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 784 GGCGCC GGC^GCC
SgrAI 135 CGCCGGCG C[GA]^CCGG[CT]G
SphI 414 GCATGC GCATG^C
Sse8387I 507 CCTGCAGG CCTGCA^GG
TaqI 410 TCGA T^CGA
Tru9I 707 TTAA T^TAA
Tsp45I 386 GTGAC ^GT[GC]AC
TthHB8I 410 TCGA T^CGA
XhoII 128 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AvaI 324 CCCGAG C^[CT]CG[GA]G
426 CTCGGG
BbrPI 183 CACGTG CAC^GTG
580 CACGTG
BcnI 331 CCCGG CC^[GC]GG
682 CCCGG
BglI 188 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
466 GCCGTGCAGGC
BpmI 271 CTCCAG CTGGAG(16/14)
586 CTGGAG
BsaAI 183 CACGTG [CT]AC^GT[GA]
580 CACGTG
BsoBI 324 CCCGAG C^[CT]CG[GA]G
426 CTCGGG
BssSI 315 CACGAG CACGAG(-5/-1)
444 CACGAG
BstXI 182 CCACGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
209 CCAGGCCTGTGG
DdeI 223 CTGAG C^T[ACGT]AG
755 CTGAG
DpnII 129 GATC ^GATC
538 GATC
EarI 246 GAAGAG CTCTTC(1/4)
261 GAAGAG
HaeII 747 GGCGCT [GA]GCGC^[CT]
784 GGCGCC
HphI 375 GGTGA GGTGA(8/7)
633 GGTGA
Ksp632I 246 GAAGAG CTCTTC(1/4)
261 GAAGAG
MaeIII 364 GTAAC ^GT[ACGT]AC
386 GTGAC
MboI 129 GATC ^GATC
538 GATC
MboII 246 GAAGA GAAGA(8/7)
261 GAAGA
MslI 486 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
555 CATCTCTGTG
MspA1I 282 CAGCGG C[AC]G^C[GT]G
592 CAGCGG
NaeI 136 GCCGGC GCC^GGC
397 GCCGGC
NciI 331 CCCGG CC^[GC]GG
682 CCCGG
NdeII 129 GATC ^GATC
538 GATC
NgoMIV 136 GCCGGC G^CCGGC
397 GCCGGC
NspI 197 GCATGT [GA]CATG^[CT]
414 GCATGC
PmaCI 183 CACGTG CAC^GTG
580 CACGTG
PmlI 183 CACGTG CAC^GTG
580 CACGTG
Sau3AI 129 GATC ^GATC
538 GATC
StuI 211 AGGCCT AGG^CCT
725 AGGCCT
TspRI 575 CACTG CA[GC]TG[ACGT][ACGT]^
612 CACTG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BmyI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrFI BssKI BstAPI BstNI
Cac8I CfoI Cfr10I Cfr13I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MnlI MspI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI SfaNI TseI