Restriction map for
pFN21AE3106
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
DraIII Eam1105I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI MaeI
MaeII MamI MboI MfeI MflI
MluI MroI MslI MunI NdeI
NdeII NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AlwNI 808 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 113 AGCGCT AGC^GCT
BalI 353 TGGCCA TGG^CCA
BbsI 763 GTCTTC GAAGAC(2/6)
BbvCI 575 CCTCAGC CCTCAGC(-5/-2)
BlpI 109 GCTGAGC GC^T[ACGT]AGC
BpmI 469 CTCCAG CTGGAG(16/14)
Bpu1102I 109 GCTGAGC GC^T[ACGT]AGC
BpuAI 763 GTCTTC GAAGAC(2/6)
BsiEI 781 CGGCCG CG[GA][CT]^CG
CelII 109 GCTGAGC GC^T[ACGT]AGC
DrdI 754 GACACCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 781 CGGCCG C^GGCCG
EclXI 781 CGGCCG C^GGCCG
Eco47III 113 AGCGCT AGC^GCT
Eco52I 781 CGGCCG C^GGCCG
EcoT14I 204 CCATGG C^C[AT][AT]GG
FseI 29 GGCCGGCC GGCCGG^CC
HinfI 493 GAGTC G^A[ACGT]TC
HphI 16 GGTGA GGTGA(8/7)
MaeIII 17 GTGAC ^GT[ACGT]AC
MluNI 353 TGGCCA TGG^CCA
MscI 353 TGGCCA TGG^CCA
MseI 791 TTAA T^TAA
NcoI 204 CCATGG C^CATGG
NspI 722 GCATGC [GA]CATG^[CT]
PleI 493 GAGTC GAGTC(4/5)
PvuII 415 CAGCTG CAG^CTG
SfaNI 710 GCATC GCATC(5/9)
SmaI 180 CCCGGG CCC^GGG
SphI 722 GCATGC GCATG^C
StyI 204 CCATGG C^C[AT][AT]GG
TaqI 210 TCGA T^CGA
Tru9I 791 TTAA T^TAA
Tsp45I 17 GTGAC ^GT[GC]AC
TthHB8I 210 TCGA T^CGA
XmaCI 180 CCCGGG C^CCGGG
XmaI 180 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 509 GTAC GT^AC
597 GTAC
ApaI 122 GGGCCC GGGCC^C
183 GGGCCC
BanI 22 GGCGCC G^G[CT][GA]CC
71 GGCGCC
BbeI 22 GGCGCC GGCGC^C
71 GGCGCC
BsaI 400 GAGACC GGTCTC(1/5)
535 GAGACC
BspMI 556 ACCTGC ACCTGC(4/8)
773 ACCTGC
BsrFI 30 GCCGGC [GA]^CCGG[CT]
544 GCCGGC
Cfr10I 30 GCCGGC [GA]^CCGG[CT]
544 GCCGGC
EarI 690 CTCTTC CTCTTC(1/4)
645 GAAGAG
EcoNI 219 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
525 CCTGAGCGAGG
HgaI 460 GACGC GACGC(5/10)
64 GCGTC
KasI 22 GGCGCC G^GCGCC
71 GGCGCC
Ksp632I 690 CTCTTC CTCTTC(1/4)
645 GAAGAG
KspI 155 CCGCGG CCGC^GG
784 CCGCGG
NaeI 30 GCCGGC GCC^GGC
544 GCCGGC
NarI 22 GGCGCC GG^CGCC
71 GGCGCC
NgoMIV 30 GCCGGC G^CCGGC
544 GCCGGC
PpuMI 473 AGGACCT [GA]G^G[AT]CC[CT]
770 GGGACCT
RsaI 509 GTAC GT^AC
597 GTAC
SacII 155 CCGCGG CCGC^GG
784 CCGCGG
SfoI 22 GGCGCC GGC^GCC
71 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanII BbvI BcnI BglI
BmyI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
ItaI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TseI TspRI