Restriction map for
pFN21AE3242
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BbeI BbvCI BclI BfrI
BglII BlpI Bpu1102I BsaBI BsaHI
BsaI BseAI BsiEI BsiWI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BstEII BstPI BstUI
CelII CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PpuMI PshBI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AclI 1079 AACGTT AA^CGTT
AflIII 656 ACACGT A^C[GA][CT]GT
AocI 218 CCTCAGG CC^T[ACGT]AGG
ApaI 95 GGGCCC GGGCC^C
BalI 1548 TGGCCA TGG^CCA
BamHI 70 GGATCC G^GATCC
BbrPI 657 CACGTG CAC^GTG
BbsI 856 GTCTTC GAAGAC(2/6)
BcgI 1323 GCAGAAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 886 GGATAC GTATCC(6/5)
BpuAI 856 GTCTTC GAAGAC(2/6)
BsaWI 394 ACCGGA [AT]^CCGG[AT]
BsgI 410 CTGCAC GTGCAG(16/14)
BsmBI 453 GAGACG CGTCTC(1/5)
BspDI 1391 ATCGAT AT^CGAT
BsrBI 931 GAGCGG CCGCTC(-3/-3)
BsrFI 1568 GCCGGT [GA]^CCGG[CT]
Bst1107I 1501 GTATAC GTA^TAC
BstAPI 738 GCAAGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 641 TTCGAA TT^CGAA
BstXI 1152 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 70 GGATCC [GA]^GATC[CT]
BstZ17I 1501 GTATAC GTA^TAC
Bsu36I 218 CCTCAGG CC^T[ACGT]AGG
CfoI 111 GCGC GCG^C
Cfr10I 1568 GCCGGT [GA]^CCGG[CT]
ClaI 1391 ATCGAT AT^CGAT
DrdI 1600 GACTCTCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 218 CCTCAGG CC^T[ACGT]AGG
EcoNI 748 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 555 GATATC GAT^ATC
HhaI 111 GCGC GCG^C
HinP1I 111 GCGC G^CGC
HincII 1347 GTTGAC GT[CT]^[GA]AC
HindII 1347 GTTGAC GT[CT]^[GA]AC
HphI 277 TCACC GGTGA(8/7)
MflI 70 GGATCC [GA]^GATC[CT]
MluNI 1548 TGGCCA TGG^CCA
MscI 1548 TGGCCA TGG^CCA
MslI 1682 CACCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1507 CAGCTG C[AC]G^C[GT]G
NcoI 537 CCATGG C^CATGG
NspI 1587 GCATGT [GA]CATG^[CT]
NspV 641 TTCGAA TT^CGAA
PflMI 121 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 657 CACGTG CAC^GTG
PmlI 657 CACGTG CAC^GTG
Psp1406I 1079 AACGTT AA^CGTT
PvuII 1507 CAGCTG CAG^CTG
SacI 769 GAGCTC GAGCT^C
SapI 1279 GAAGAGC GCTCTTC(1/4)
SexAI 1628 ACCAGGT A^CC[AT]GGT
SfuI 641 TTCGAA TT^CGAA
Van91I 121 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 70 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 71 GATCC GGATC(4/5)
70 GGATC
AvrII 477 CCTAGG C^CTAGG
1464 CCTAGG
BlnI 477 CCTAGG C^CTAGG
1464 CCTAGG
BsaAI 657 CACGTG [CT]AC^GT[GA]
1651 TACGTG
BsmI 669 GAATGC GAATGC(1/-1)
1296 GAATGC
BssSI 1375 CTCGTG CACGAG(-5/-1)
1604 CTCGTG
Cac8I 97 GCCCGC GC[ACGT]^[ACGT]GC
893 GCCAGC
DpnII 71 GATC ^GATC
253 GATC
DsaI 387 CCGTGG C^C[GA][CT]GG
537 CCATGG
EaeI 163 CGGCCA [CT]^GGCC[GA]
1548 TGGCCA
EarI 704 CTCTTC CTCTTC(1/4)
1279 GAAGAG
HgaI 357 GCGTC GACGC(5/10)
1538 GCGTC
Ksp632I 704 CTCTTC CTCTTC(1/4)
1279 GAAGAG
MboI 71 GATC ^GATC
253 GATC
MseI 50 TTAA T^TAA
1045 TTAA
NdeII 71 GATC ^GATC
253 GATC
PstI 679 CTGCAG CTGCA^G
1450 CTGCAG
Sau3AI 71 GATC ^GATC
253 GATC
SfcI 679 CTGCAG C^T[GA][CT]AG
1450 CTGCAG
SmlI 187 CTCAAG C^T[CT][GA]AG
1144 CTCAAG
StuI 8 AGGCCT AGG^CCT
1154 AGGCCT
Tru9I 50 TTAA T^TAA
1045 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccI AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeI MaeII
MaeIII MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV PleI
PshAI RsaI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I