Restriction map for
pFN21AE3685
##################################
# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI RcaI RsaI RsrII
SacI SacII SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3 GTCGAC GT^[AC][GT]AC
AclI 740 AACGTT AA^CGTT
AcsI 12 GAATTT [GA]^AATT[CT]
ApoI 12 GAATTT [GA]^AATT[CT]
AspI 599 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 792 GGGCTC G[GA]GC[CT]^C
BbvI 221 GCAGC GCAGC(8/12)
BpmI 777 CTCCAG CTGGAG(16/14)
BsaJI 180 CCGAGG C^C[ACGT][ACGT]GG
BsiYI 773 CCAGCTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 773 CCAGCTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspDI 95 ATCGAT AT^CGAT
BssSI 752 CTCGTG CACGAG(-5/-1)
BstEII 917 GGTCACC G^GT[ACGT]ACC
BstPI 917 GGTCACC G^GT[ACGT]ACC
BstXI 930 CCAGGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 95 ATCGAT AT^CGAT
EcoO65I 917 GGTCACC G^GT[ACGT]ACC
Fnu4HI 221 GCAGC GC^[ACGT]GC
HgaI 529 GACGC GACGC(5/10)
HincII 3 GTCGAC GT[CT]^[GA]AC
HindII 3 GTCGAC GT[CT]^[GA]AC
ItaI 221 GCAGC GC^[ACGT]GC
MspA1I 87 CAGCTG C[AC]G^C[GT]G
PflFI 599 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 20 GAGTC GAGTC(4/5)
Psp1406I 740 AACGTT AA^CGTT
PvuII 87 CAGCTG CAG^CTG
SalI 3 GTCGAC G^TCGAC
SapI 794 GCTCTTC GCTCTTC(1/4)
SmlI 660 CTCAAG C^T[CT][GA]AG
StuI 183 AGGCCT AGG^CCT
TseI 221 GCAGC G^C[AT]GC
Tth111I 599 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 868 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 770 GATCC GGATC(4/5)
619 GGATC
Asp700I 695 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
860 GAAGTCGTTC
BmyI 144 GGGCAC G[AGT]GC[ACT]^C
792 GGGCTC
BsaBI 481 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
635 GATCTTCATC
Bsp1286I 144 GGGCAC G[AGT]GC[ACT]^C
792 GGGCTC
BsrDI 207 GCAATG GCAATG(2/0)
224 GCAATG
DdeI 119 CTGAG C^T[ACGT]AG
842 CTGAG
DraII 682 AGGTCCC [GA]G^G[ACGT]CC[CT]
781 AGGTCCC
EarI 795 CTCTTC CTCTTC(1/4)
469 GAAGAG
EcoO109I 682 AGGTCCC [GA]G^G[ACGT]CC[CT]
781 AGGTCCC
Ksp632I 795 CTCTTC CTCTTC(1/4)
469 GAAGAG
MaeII 384 ACGT A^CGT
741 ACGT
MaeIII 26 GTGAC ^GT[ACGT]AC
918 GTCAC
MamI 481 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
635 GATCTTCATC
MseI 317 TTAA T^TAA
433 TTAA
NlaIV 683 GGTCCC GG[ACGT]^[ACGT]CC
782 GGTCCC
PpuMI 682 AGGTCCC [GA]G^G[AT]CC[CT]
781 AGGTCCC
SfaNI 556 GATGC GCATC(5/9)
424 GCATC
TfiI 355 GAATC G^A[AT]TC
454 GAATC
Tru9I 317 TTAA T^TAA
433 TTAA
Tsp45I 26 GTGAC ^GT[GC]AC
918 GTCAC
XmnI 695 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
860 GAAGTCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AvaII BfaI BglII BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DpnII EcoRII FokI HaeIII
HinfI HphI MaeI MboI MboII
MflI MnlI MvaI NdeII Sau3AI
Sau96I ScrFI TaqI Tsp509I TspRI
TthHB8I XhoII